A big image opens when the thumbnail is clicked.(nies-0981.jpg)
Strain number NIES-981  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Cyanobium sp.  
Former name Formerly identified as Synechococcus sp., re-identified at NIES by DNA analysis[2016 Apr]  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Hoshidate, Iriomote Isl., Okinawa, Japan (1999-07-01)  
Latitude / Longitude 24.391383421726953 / 123.75227785384293 
Habitat (Isolation source) Marine (Tidal flat sediment)  
History < Tezuka, Naoaki  
Isolator (Date of isolation) Tezuka, Naoaki (1999-09-**)  
Identified by Re-identified by NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2015 Dec]  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  9-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information Whole-genome ( LT578417 ) , 16S rRNA ( AF448064 ) , rpoC1 ( AF448085 )  
Cell size (min - max) 1 - 3 μm  
Organization Unicellular 
Characteristics Test strain (Yamagishi et al. 2016) ; Genome decoded strain (Yamaguchi et al. 2016) ; Synechococcus clade group K (Suzuki et al. 2022)  
Other strain no. Other strain no. : Hos  
Remarks Cryopreserved; Axenic 
Yancey, C. E., Smith, D. J., Den Uyl, P. A., Mohamed, O. G., Yu, F., Ruberg, S. A., Chaffin, J. D., Goodwin, K. D., Tripathi, A., Sherman, D., Dick, G. J. 2022 Metagenomic and metatranscriptomic insights into population diversity of Microcystis blooms: Spatial and temporal dynamics of mcy genotypes, including a partial operon that can be abundant and expressed. Appl. Environ. Microbiol., 88, e0246421 (article ID).
Keywords: freshwater harmful algal blooms; Microcystis; metagenomics; mcy operon; Lake Erie
Strain(s): 981 
PubMed: 35438519
DOI: 10.1128/aem.02464-21

Ota, S., Fuchida, S., Yamaguchi, H., Yamagishi, T., Yamamoto, H., Koshikawa, H., Kawachi, M. 2022 Propidium iodide staining and flow cytometry-based assessment of heavy metal impact on marine phytoplankton. Cytologia, 87, 177-187.
Keywords: Cyanobacteria; Deep-sea mining; Flow cytometry; Heavy metal contaminant; Microalgae; On-chip sort
Strain(s): 9699812670 
DOI: 10.1508/cytologia.87.177

Suzuki, S., Yamaguchi, H., Kawachi, M. 2022 Potential genetic diversity of Synechococcus-related strains maintained in the Microbial Culture Collection of the National Institute for Environmental Studies, Japan. Microb. Resour. Syst., 38, 63-74.
Keywords: Synechococcus; Cyanobium; picoplankton; molecular marker
Strain(s): 93793893994094194294394594694794995095195295395495595695795895996096196296396496596997097197297397497597697797897998198298398498598698798813411342134313441345134613471348 

Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH4+ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611.
Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism
Strain(s): 25502042678439709812107213421352481254937093754375637574074 
PubMed: 35595516
DOI: 10.1093/jxb/erac219

Chernomor, O., Peters, L., Schneidewind, J., Loeschcke, A., Knieps-Grünhagen, E., Schmitz, F., von Lieres, E.,Kutta, R. J., Svensson, V., Jaeger, K., Drepper, T.,von Haeseler, A., Krauss, U. 2021 Complex evolution of light-dependent protochlorophyllide oxidoreductases in aerobic anoxygenic phototrophs: origin, phylogeny and function. Mol. Biol. Evol., 38, 819-837.
Keywords: light-driven enzyme; chlorophyll biosynthesis; evolution; aerobic anoxygenic phototrophic bacteria; photosynthesis
Strain(s): 9812135 
PubMed: 32931580
DOI: 10.1093/molbev/msaa234

Ota, S., Yamaguchi, H., Vanel, F., Fuchida, S., Koshikawa, H., Yamagishi, T., Yamamoto, H., Kawachi, M. 2020 Differential heavy metal sensitivity in seven algal species from the NIES culture collection based on delayed fluorescence assays. Phycol. Res., 68, 41-49.
Strain(s): 9699719811001131025872670 
DOI: 10.1111/pre.12403

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27394273818789100101102103104105107111112207208230266298416433478510512515592593604611808809835843846847905928931932945981989102510431058106110641068106910711076108510991101110511131115112611331134113511441157121516421643165216681672167316771678169820872131241226653708373337343736375537563757 
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Yamagishi, T., Ota, S., Yamaguchi, H., Koshikawa, H., Tatarazako, N., Yamamoto, H., Kawachi, M. 2019 Ecotoxicological bioassay using marine algae for deep-sea mining. In Environmental Issues of Deep-Sea Mining: Impacts, Consequences and Policy Perspectives, Ed. by Sharma, R., Springer, Cham, pp. 255-271.
Strain(s): 323613969971981100010011310141123042587259026702672 
DOI: 10.1007/978-3-030-12696-4_10

Yamagishi, T., Fuchida, S., Katsumata, M., Horie, Y., Mori, F., Kitayama, A., Kawachi, M., Koshikawa, H., Nozaki, T., Kumagai, H., Ishibashi, J-I., Tatarazako, N. 2018 Evaluation of the toxicity of leaches from hydrothermal sulfide deposits by means of a delayed fluorescence-based bioassay with the marine cyanobacterium Cyanobium sp. NIES-981. Ecotoxicology, 27, 1303–1309.
Keywords: Cyanobium sp.; Deep-sea mining; Delayed fluorescence; Ecotoxicological bioassay; Heavy metal
Strain(s): 981 
PubMed: 30311132
DOI: 10.1007/s10646-018-1989-2

Yamaguchi, H., Shimura, Y., Suzuki, S., Yamagishi, T., Tatarazako, N., Kawachi, M. 2016 Complete genome sequence of Cyanobium sp. NIES-981, a marine strain potentially useful for ecotoxicological bioassays. GenomeA, 4, e00736-16 (article ID).
Strain(s): 981 
PubMed: 27469961
DOI: 10.1128/genomeA.00736-16

Yamagishi, T., Katsumata, M., Yamaguchi, H., Shimura, Y., Kawachi, M., Koshikawa, H., Horie, Y., Tatarazako, N. 2016 Rapid ecotoxicological bioassay using delayed fluorescence in the marine cyanobacterium Cyanobium sp. (NIES-981). Ecotoxicology, 25, 1751-1758.
Keywords: Algal growth inhibition test; Cyanobium sp.; Delayed fluorescence; Marine cyanobacteria
Strain(s): 981 
PubMed: 27633095
DOI: 10.1007/s10646-016-1718-7

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