Strain Data

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Strain number		NIES-981
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Cyanobium sp.
Synonym
Former name		Formerly identified as Synechococcus sp., re-identified at NIES by DNA analysis[2016 Apr]
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Hoshidate, Iriomote Isl., Okinawa, Japan (1999-07-01)
Latitude / Longitude		24.391383421726953 / 123.75227785384293
Habitat (Isolation source)		Marine (Tidal flat sediment)
History		< Tezuka, Naoaki
Isolator (Date of isolation)		Tezuka, Naoaki (1999-09-**)
Identified by		Re-identified by NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2015 Dec]
Culture condition (Preculture condition)		Medium: ESM Temperature: 20 C Light intensity: 9-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		Whole-genome ( LT578417 ) , 16S rRNA ( AF448064 ) , rpoC1 ( AF448085 )
Cell size (min - max)		1 - 3 μm
Organization		Unicellular
Characteristics		Test strain (Yamagishi et al. 2016) ; Genome decoded strain (Yamaguchi et al. 2016) ; Synechococcus clade group K (Suzuki et al. 2022)
Other strain no.		Other strain no. : Hos
Remarks		Cryopreserved; Axenic
Movie

Reference
Yancey, C. E., Smith, D. J., Den Uyl, P. A., Mohamed, O. G., Yu, F., Ruberg, S. A., Chaffin, J. D., Goodwin, K. D., Tripathi, A., Sherman, D., Dick, G. J. 2022 Metagenomic and metatranscriptomic insights into population diversity of Microcystis blooms: Spatial and temporal dynamics of mcy genotypes, including a partial operon that can be abundant and expressed. Appl. Environ. Microbiol., 88, e0246421 (article ID). Keywords: freshwater harmful algal blooms; Microcystis; metagenomics; mcy operon; Lake Erie Strain(s): 981 PubMed: 35438519 DOI: 10.1128/aem.02464-21 Ota, S., Fuchida, S., Yamaguchi, H., Yamagishi, T., Yamamoto, H., Koshikawa, H., Kawachi, M. 2022 Propidium iodide staining and flow cytometry-based assessment of heavy metal impact on marine phytoplankton. Cytologia, 87, 177-187. Keywords: Cyanobacteria; Deep-sea mining; Flow cytometry; Heavy metal contaminant; Microalgae; On-chip sort Strain(s): 969, 981, 2670 DOI: 10.1508/cytologia.87.177 Suzuki, S., Yamaguchi, H., Kawachi, M. 2022 Potential genetic diversity of Synechococcus-related strains maintained in the Microbial Culture Collection of the National Institute for Environmental Studies, Japan. Microb. Resour. Syst., 38, 63-74. Keywords: Synechococcus; Cyanobium; picoplankton; molecular marker Strain(s): 937, 938, 939, 940, 941, 942, 943, 945, 946, 947, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959, 960, 961, 962, 963, 964, 965, 969, 970, 971, 972, 973, 974, 975, 976, 977, 978, 979, 981, 982, 983, 984, 985, 986, 987, 988, 1341, 1342, 1343, 1344, 1345, 1346, 1347, 1348 Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611. Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074 PubMed: 35595516 DOI: 10.1093/jxb/erac219 Chernomor, O., Peters, L., Schneidewind, J., Loeschcke, A., Knieps-Grünhagen, E., Schmitz, F., von Lieres, E.,Kutta, R. J., Svensson, V., Jaeger, K., Drepper, T.,von Haeseler, A., Krauss, U. 2021 Complex evolution of light-dependent protochlorophyllide oxidoreductases in aerobic anoxygenic phototrophs: origin, phylogeny and function. Mol. Biol. Evol., 38, 819-837. Keywords: light-driven enzyme; chlorophyll biosynthesis; evolution; aerobic anoxygenic phototrophic bacteria; photosynthesis Strain(s): 981, 2135 PubMed: 32931580 DOI: 10.1093/molbev/msaa234 Ota, S., Yamaguchi, H., Vanel, F., Fuchida, S., Koshikawa, H., Yamagishi, T., Yamamoto, H., Kawachi, M. 2020 Differential heavy metal sensitivity in seven algal species from the NIES culture collection based on delayed fluorescence assays. Phycol. Res., 68, 41-49. Strain(s): 969, 971, 981, 1001, 1310, 2587, 2670 DOI: 10.1111/pre.12403 Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578. Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757 PubMed: 32501712 DOI: 10.1021/jasms.0c00148 Yamagishi, T., Ota, S., Yamaguchi, H., Koshikawa, H., Tatarazako, N., Yamamoto, H., Kawachi, M. 2019 Ecotoxicological bioassay using marine algae for deep-sea mining. In Environmental Issues of Deep-Sea Mining: Impacts, Consequences and Policy Perspectives, Ed. by Sharma, R., Springer, Cham, pp. 255-271. Strain(s): 323, 613, 969, 971, 981, 1000, 1001, 1310, 1411, 2304, 2587, 2590, 2670, 2672 DOI: 10.1007/978-3-030-12696-4_10 Yamagishi, T., Fuchida, S., Katsumata, M., Horie, Y., Mori, F., Kitayama, A., Kawachi, M., Koshikawa, H., Nozaki, T., Kumagai, H., Ishibashi, J-I., Tatarazako, N. 2018 Evaluation of the toxicity of leaches from hydrothermal sulfide deposits by means of a delayed fluorescence-based bioassay with the marine cyanobacterium Cyanobium sp. NIES-981. Ecotoxicology, 27, 1303–1309. Keywords: Cyanobium sp.; Deep-sea mining; Delayed fluorescence; Ecotoxicological bioassay; Heavy metal Strain(s): 981 PubMed: 30311132 DOI: 10.1007/s10646-018-1989-2 Yamaguchi, H., Shimura, Y., Suzuki, S., Yamagishi, T., Tatarazako, N., Kawachi, M. 2016 Complete genome sequence of Cyanobium sp. NIES-981, a marine strain potentially useful for ecotoxicological bioassays. GenomeA, 4, e00736-16 (article ID). Strain(s): 981 PubMed: 27469961 DOI: 10.1128/genomeA.00736-16 Yamagishi, T., Katsumata, M., Yamaguchi, H., Shimura, Y., Kawachi, M., Koshikawa, H., Horie, Y., Tatarazako, N. 2016 Rapid ecotoxicological bioassay using delayed fluorescence in the marine cyanobacterium Cyanobium sp. (NIES-981). Ecotoxicology, 25, 1751-1758. Keywords: Algal growth inhibition test; Cyanobium sp.; Delayed fluorescence; Marine cyanobacteria Strain(s): 981 PubMed: 27633095 DOI: 10.1007/s10646-016-1718-7

Reference

Yancey, C. E., Smith, D. J., Den Uyl, P. A., Mohamed, O. G., Yu, F., Ruberg, S. A., Chaffin, J. D., Goodwin, K. D., Tripathi, A., Sherman, D., Dick, G. J. 2022 Metagenomic and metatranscriptomic insights into population diversity of Microcystis blooms: Spatial and temporal dynamics of mcy genotypes, including a partial operon that can be abundant and expressed. Appl. Environ. Microbiol., 88, e0246421 (article ID).
Keywords: freshwater harmful algal blooms; Microcystis; metagenomics; mcy operon; Lake Erie
Strain(s): 981
PubMed: 35438519
DOI: 10.1128/aem.02464-21

Ota, S., Fuchida, S., Yamaguchi, H., Yamagishi, T., Yamamoto, H., Koshikawa, H., Kawachi, M. 2022 Propidium iodide staining and flow cytometry-based assessment of heavy metal impact on marine phytoplankton. Cytologia, 87, 177-187.
Keywords: Cyanobacteria; Deep-sea mining; Flow cytometry; Heavy metal contaminant; Microalgae; On-chip sort
Strain(s): 969, 981, 2670
DOI: 10.1508/cytologia.87.177

Suzuki, S., Yamaguchi, H., Kawachi, M. 2022 Potential genetic diversity of Synechococcus-related strains maintained in the Microbial Culture Collection of the National Institute for Environmental Studies, Japan. Microb. Resour. Syst., 38, 63-74.
Keywords: Synechococcus; Cyanobium; picoplankton; molecular marker
Strain(s): 937, 938, 939, 940, 941, 942, 943, 945, 946, 947, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959, 960, 961, 962, 963, 964, 965, 969, 970, 971, 972, 973, 974, 975, 976, 977, 978, 979, 981, 982, 983, 984, 985, 986, 987, 988, 1341, 1342, 1343, 1344, 1345, 1346, 1347, 1348

Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH₄⁺ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611.
Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism
Strain(s): 25, 50, 204, 267, 843, 970, 981, 2107, 2134, 2135, 2481, 2549, 3709, 3754, 3756, 3757, 4074
PubMed: 35595516
DOI: 10.1093/jxb/erac219

Chernomor, O., Peters, L., Schneidewind, J., Loeschcke, A., Knieps-Grünhagen, E., Schmitz, F., von Lieres, E.,Kutta, R. J., Svensson, V., Jaeger, K., Drepper, T.,von Haeseler, A., Krauss, U. 2021 Complex evolution of light-dependent protochlorophyllide oxidoreductases in aerobic anoxygenic phototrophs: origin, phylogeny and function. Mol. Biol. Evol., 38, 819-837.
Keywords: light-driven enzyme; chlorophyll biosynthesis; evolution; aerobic anoxygenic phototrophic bacteria; photosynthesis
Strain(s): 981, 2135
PubMed: 32931580
DOI: 10.1093/molbev/msaa234

Ota, S., Yamaguchi, H., Vanel, F., Fuchida, S., Koshikawa, H., Yamagishi, T., Yamamoto, H., Kawachi, M. 2020 Differential heavy metal sensitivity in seven algal species from the NIES culture collection based on delayed fluorescence assays. Phycol. Res., 68, 41-49.
Strain(s): 969, 971, 981, 1001, 1310, 2587, 2670
DOI: 10.1111/pre.12403

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Yamagishi, T., Ota, S., Yamaguchi, H., Koshikawa, H., Tatarazako, N., Yamamoto, H., Kawachi, M. 2019 Ecotoxicological bioassay using marine algae for deep-sea mining. In Environmental Issues of Deep-Sea Mining: Impacts, Consequences and Policy Perspectives, Ed. by Sharma, R., Springer, Cham, pp. 255-271.
Strain(s): 323, 613, 969, 971, 981, 1000, 1001, 1310, 1411, 2304, 2587, 2590, 2670, 2672
DOI: 10.1007/978-3-030-12696-4_10

Yamagishi, T., Fuchida, S., Katsumata, M., Horie, Y., Mori, F., Kitayama, A., Kawachi, M., Koshikawa, H., Nozaki, T., Kumagai, H., Ishibashi, J-I., Tatarazako, N. 2018 Evaluation of the toxicity of leaches from hydrothermal sulfide deposits by means of a delayed fluorescence-based bioassay with the marine cyanobacterium Cyanobium sp. NIES-981. Ecotoxicology, 27, 1303–1309.
Keywords: Cyanobium sp.; Deep-sea mining; Delayed fluorescence; Ecotoxicological bioassay; Heavy metal
Strain(s): 981
PubMed: 30311132
DOI: 10.1007/s10646-018-1989-2

Yamaguchi, H., Shimura, Y., Suzuki, S., Yamagishi, T., Tatarazako, N., Kawachi, M. 2016 Complete genome sequence of Cyanobium sp. NIES-981, a marine strain potentially useful for ecotoxicological bioassays. GenomeA, 4, e00736-16 (article ID).
Strain(s): 981
PubMed: 27469961
DOI: 10.1128/genomeA.00736-16

Yamagishi, T., Katsumata, M., Yamaguchi, H., Shimura, Y., Kawachi, M., Koshikawa, H., Horie, Y., Tatarazako, N. 2016 Rapid ecotoxicological bioassay using delayed fluorescence in the marine cyanobacterium Cyanobium sp. (NIES-981). Ecotoxicology, 25, 1751-1758.
Keywords: Algal growth inhibition test; Cyanobium sp.; Delayed fluorescence; Marine cyanobacteria
Strain(s): 981
PubMed: 27633095
DOI: 10.1007/s10646-016-1718-7

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