Strain Data

Strain number		NIES-928
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Planktothrix rubescens (DC. ex Gomont) Anagnostidis & Komárek
Synonym		Oscillatoria rubescens DC. ex Gomont
Former name
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Cambria, England, U.K. (1975--)
Latitude / Longitude
Habitat (Isolation source)		Freshwater
History		< Suda, Shoichiro < CCAP
Isolator (Date of isolation)		Jaworski, G. H. M. (1975--)
Identified by		Suda, Shoichiro (Reidentify)
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: CT Temperature: 20 C Light intensity: 22-30 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		16S rRNA ( FJ184422 GQ995000 ) , 16S-23S rRNA ( AB686456 GQ995018 ) , gyrB ( GQ995043 ) , mcyE ( AB638214 ) , rpoB ( GQ995009 ) , rpoC1 ( FJ173023 GQ995068 ) , rbcL ( FJ184476 )
Cell size (min - max)
Organization		Filamentous
Characteristics		Cyanobacterial water bloom (aoko) ; Toxicity ; Planktonic
Other strain no.		Other collection strain no. : CCAP 1459/14
Remarks		Cryopreserved; Toxicity
Movie

Reference
Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578. Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757 PubMed: 32501712 DOI: 10.1021/jasms.0c00148 Gaget, V., Welker, M., Rippka, R., de Marsac, N. T. 2015 A polyphasic approach leading to the revision of the genus Planktothrix (Cyanobacteria) and its type species, P. agardhii, and proposal for integrating the emended valid botanical taxa, as well as three new species, Planktothrix paucivesiculata sp. nov.^ICNP, Planktothrix tepida sp. nov.^ICNP, Planktothrix serta sp. nov.^ICNP, as genus and species names with nomenclatural standing under th ICNP. Syst. Appl. Microbiol., 38, 141-158. Keywords: Polyphasic taxonomy; 16S rRNA gene; ITS; MLST; gyrB; rpoB; rpoC1; Planktothrix Anagnostidis & Komárek 1988^ICN emend. gen. nov. ^ICNP Strain(s): 905, 928, 1266 DOI: 10.1016/j.syapm.2015.02.004 Shimizu, K., Sano, T., Kubota, R., Kobayashi, N., Tahara, M., Obama, T., Sugimoto, N., Nishimura, T., Ikarashi, Y. 2014 Effects of the amino acid constituents of microcystin variants on cytotoxicity to primary cultured rat hepatocytes. Toxins, 6, 168-179. Keywords: microcystin; variants; cytotoxicity; primary cultured rat hepatocytes; environmental water Strain(s): 90, 595, 610, 928 PubMed: 24380975 DOI: 10.3390/toxins6010168 Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57. Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa Strain(s): 30, 40, 77, 102, 111, 230, 512, 843, 905, 928, 932, 937, 991, 1645, 1669, 1698, 1729, 1950, 2116 DOI: 10.1016/j.hal.2012.01.003 Lin, S., Wu, Z., Yu, G., Zhu, M., Yu, B., Li, R. 2010 Genetic diversity and molecular phylogeny of Planktothrix (Oscillatoriales, cyanobacteria) strains from China. Harmful Algae, 9, 87-97. Keywords: diversity; phylogeny; Planktothrix; 16S rRNA; rbcLX; rpoC1; taxonomy; water bloom Strain(s): 928, 1266 DOI: 10.1016/j.hal.2009.08.004 Sano, T., Takagi, H., Kaya, K. 2004 A Dhb-microcystin from the filamentous cyanobacterium Planktothrix rubescens. Phytochemistry, 65, 2159-2162. Keywords: Planktothrix rubescens; cyanobacterium; microcystin; Dhb-microcystin; hepatotoxin; cyanotoxin Strain(s): 928 DOI: 10.1016/j.phytochem.2004.03.034 Sano, T., Usui, T., Ueda, K., Osada, H., Kaya, K. 2001 Isolation of new protein phosphatase inhibitors from two cyanobacteria species, Planktothrix spp. J. Nat. Prod., 64, 1052-1055. Strain(s): 905, 928 PubMed: 11520225 DOI: 10.1021/np0005356

Reference

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Gaget, V., Welker, M., Rippka, R., de Marsac, N. T. 2015 A polyphasic approach leading to the revision of the genus Planktothrix (Cyanobacteria) and its type species, P. agardhii, and proposal for integrating the emended valid botanical taxa, as well as three new species, Planktothrix paucivesiculata sp. nov.^ICNP, Planktothrix tepida sp. nov.^ICNP, Planktothrix serta sp. nov.^ICNP, as genus and species names with nomenclatural standing under th ICNP. Syst. Appl. Microbiol., 38, 141-158.
Keywords: Polyphasic taxonomy; 16S rRNA gene; ITS; MLST; gyrB; rpoB; rpoC1; Planktothrix Anagnostidis & Komárek 1988^ICN emend. gen. nov. ^ICNP
Strain(s): 905, 928, 1266
DOI: 10.1016/j.syapm.2015.02.004

Shimizu, K., Sano, T., Kubota, R., Kobayashi, N., Tahara, M., Obama, T., Sugimoto, N., Nishimura, T., Ikarashi, Y. 2014 Effects of the amino acid constituents of microcystin variants on cytotoxicity to primary cultured rat hepatocytes. Toxins, 6, 168-179.
Keywords: microcystin; variants; cytotoxicity; primary cultured rat hepatocytes; environmental water
Strain(s): 90, 595, 610, 928
PubMed: 24380975
DOI: 10.3390/toxins6010168

Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 30, 40, 77, 102, 111, 230, 512, 843, 905, 928, 932, 937, 991, 1645, 1669, 1698, 1729, 1950, 2116
DOI: 10.1016/j.hal.2012.01.003

Lin, S., Wu, Z., Yu, G., Zhu, M., Yu, B., Li, R. 2010 Genetic diversity and molecular phylogeny of Planktothrix (Oscillatoriales, cyanobacteria) strains from China. Harmful Algae, 9, 87-97.
Keywords: diversity; phylogeny; Planktothrix; 16S rRNA; rbcLX; rpoC1; taxonomy; water bloom
Strain(s): 928, 1266
DOI: 10.1016/j.hal.2009.08.004

Sano, T., Takagi, H., Kaya, K. 2004 A Dhb-microcystin from the filamentous cyanobacterium Planktothrix rubescens. Phytochemistry, 65, 2159-2162.
Keywords: Planktothrix rubescens; cyanobacterium; microcystin; Dhb-microcystin; hepatotoxin; cyanotoxin
Strain(s): 928
DOI: 10.1016/j.phytochem.2004.03.034

Sano, T., Usui, T., Ueda, K., Osada, H., Kaya, K. 2001 Isolation of new protein phosphatase inhibitors from two cyanobacteria species, Planktothrix spp. J. Nat. Prod., 64, 1052-1055.
Strain(s): 905, 928
PubMed: 11520225
DOI: 10.1021/np0005356

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