Strain Data

A big image opens when the thumbnail is clicked.(nies-0837-1.jpg)

A big image opens when the thumbnail is clicked.(nies-0837-2.jpg)

A big image opens when the thumbnail is clicked.(nies-0837-3.jpg)

Strain number		NIES-837
Phylum		Haptophyta
Class		Coccolithophyceae
Scientific name		Emiliania huxleyi (Lohmann) W.W.Hay & H.Mohler
Synonym		Pontosphaera huxleyi Lohmann 1902; Hymenomonas huxleyi (Lohmann) Kamptner 1930; Coccolithus huxleyi (Lohmann) Kamptner 1943; Gephyrocapsa huxleyi (Lohmann) Reinhardt 1972; Gephyrocapsa huxleyi P.Reinhardt 1972
Former name
Common name		Coccolithophore
Locality (Date of collection)		Great Barrier Reef, Australia (1990-11-**)
Latitude / Longitude
Habitat (Isolation source)		Marine (Seawater)
History		< Kawachi, Masanobu
Isolator (Date of isolation)		Inouye, Isao (1990-11-**)
Identified by		Inouye, Isao
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic; Coccolith(+/-)[2014 Nov]
Culture condition (Preculture condition)		Medium: MNK ; mIMR Temperature: 20 C Light intensity: 19 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		CO1b, atp4 ( AB563768 ) , EhPEPC ( AB461362 ) , EhPYC ( AB461363 ) , EhSEP2 ( AB205027 )
Cell size (min - max)		3 - 6 μm
Organization		Unicellular
Characteristics		Red tide ; Planktonic ; Biomineralization (Kayano et al. 2011) ; clade O (Hagino et al. 2011)
Other strain no.		Other strain no. : EH-2
Remarks		Cryopreserved; Unstable
Movie

Reference
Yoneda, K., Kobayashi, C., Araie, H., Morita, R., Harada, R., Shigeta, Y., Endo, H., Maeda, Y., Suzuki, I. 2025 Characterization of delta-7 alkenone desaturase in haptophyte Gephyrocapsa huxleyi through heterologous expression in Tisochrysis lutea. Mar. Biotechnol., 27, 44 (article ID). Keywords: Gephyrocapsa huxleyi; Alkenones; Desaturase; Transformation; Heterologous expression; Tisochrysis lutea Strain(s): 837 PubMed: 39921736 DOI: 10.1007/s10126-025-10427-y Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 Pelusi, A., Hanawa, Y., Araie, H., Suzuki, I., Giordan, M., Shiraiwa, Y. 2016 Rapid detection and quantification of haptophyte alkenones by Fourier transform infrared spectroscopy (FTIR). Algal Res., 19, 48-56. Keywords: Alkenones; Alkenone-producing haptophyte; Emiliania huxleyi; FTIR; Lipid;trans-Carbon-carbon double bond Strain(s): 837 DOI: 10.1016/j.algal.2016.07.006 Nakamura, H., Sawada, K., Araie, H., Suzuki, I., Shiraiwa, Y. 2015 n-Nonacosadienes from the marine haptophytes Emiliania huxleyi and Gephyrocapsa oceanica. Phytochemistry, 111, 107-113. Keywords: Emiliania huxleyi; Gephyrocapsa oceanica; Haptophyte; Nonacosadiene; Noelaerhabdaceae; Long chain alkene; Biomarker Strain(s): 837, 1315 PubMed: 25595675 DOI: 10.1016/j.phytochem.2014.12.023 Tsuji, Y., Yamazaki, M., Suzuki, I., Shiraiwa, Y. 2015 Quantitative analysis of carbon flow into photosynthetic products functioning as carbon storage in the marine coccolithophore, Emiliania huxleyi. Mar. Biotechnol., 17, 428-440. Keywords: Alkenones; Carbon partitioning; Carbon storage compound; Emiliania huxleyi; Haptophyte; Lipid; biosynthesis Strain(s): 837 PubMed: 14730424 DOI: 10.1007/s10126-002-0081-2 Fukuda, S., Suzuki, Y., Shiraiwa, Y. 2014 Difference in physiological responses of growth, photosynthesis and calciﬁcation of the coccolithophore Emiliania huxleyi to acidiﬁcation by acid and CO₂ enrichment. Photosynth. Res., 121, 299-309. Keywords: Acidiﬁcation; CO₂ enrichment; Calciﬁcation; Emiliania huxleyi; pH effect; Photosynthesis Strain(s): 837 PubMed: 24500605 DOI: 10.1007/s11120-014-9976-9 Yamane, K., Matsuyama, S., Igarashi, K., Utsumi, M., Shiraiwa, Y., Kuwabara, T. 2013 Anaerobic coculture of microalgae with Thermosipho globiformans and Methanocaldococcus jannaschii at 68 ºC enhances generarion of n-alkane-rich biofules after pyrolysis. Appl. Environ. Microbiol., 79, 924-930. Strain(s): 837, 2258 PubMed: 23183975 DOI: 10.1128/AEM.01685-12 Yamane, K., Matsuyama, S., Igarashi, K., Kuwabara, T. 2013 Pyrolytic Generation of Petroleum Crude Oils from the Marine Phytomicroalgal Coccolithophore Emiliania huxleyi (Haptophyta) and Preparation of n-Alkane-Rich Biofuel. Energy Fuels, 27, 7470–7479. Strain(s): 837 DOI: 10.1021/ef401618t Tsuji, Y., Suzuki, I., Shiraiwa, Y. 2012 Enzymological evidence for the function of a plastid-located pyruvate carboxylase in the Haptophyte alga Emiliania huxleyi: a novel pathway for the production of C ₄ compounds. Plant Cell Physiol., 53, 1043-1052. Strain(s): 837 PubMed: 22492231 DOI: 10.1093/pcp/pcs045 Iwamoto, K. & Shiraiwa, Y. 2012 Characterization of intracellular iodine accumulation by iodine-tolerant microalgae. Procedia Environ. Sco., 15, 34-42. Keywords: growth stimulation; growth inhibition; haptophyte; iodine; iodine accumulation Strain(s): 377, 837, 838 DOI: 10.1016/j.proenv.2012.05.007 Mizoguchi, T., Kimura, Y., Yoshitomi, T., Tamiaki, H. 2011 The stereochemistry of chlorophyll-c₃ from the haptophyte Emiliania huxleyi: the (13²R)-enantiomers of chlorophylls-c are exclusively selected as the photosynthetically active pigments in chromophyte algae. Biochim. Biophys. Acta-Bioenerg., 1807, 1467-1473. Keywords: chlorophyll-c; chiral HPLC; enantioimer; chromophyte algae; light-harvesting complexes Strain(s): 71, 281, 696, 837 PubMed: 21806961 DOI: 10.1016/j.bbabio.2011.07.008 Kayano, K., Saruwatari, K., Kogure, T., Shiraiwa, Y. 2011 Effect of coccolith polysaccharides isolated from the coccolithophorid, Emiliania huxleyi, on calcite crystal formation in in vitro CaCO₃ crystallization. Mar. Biotechnol., 13, 83-92. Keywords: Acid polysaccharide; Biomineralization; Coccolith; Coccolith polysaccharide; Calcium carbonate crystalization; Emiliania huxleyi Strain(s): 837 PubMed: 20336339 DOI: 10.1007/s10126-010-9272-4 Hagino, K., Bendif, E. M., Young, J. R. Kogame, K., Probert, I., Takano, Y., Horiguchi, T., de Vardas, C., Okada, H. 2011 New evidence for morphological and genetic variation in the cosmopolitan coccolithophore Emiliania huxleyi (Prymnesiophyceae) from the COX1b-ATP4 genes. J. Phycol., 47, 1164-1176. Keywords: biogeography; coccolithophore; Emiliania huxleyi; mitochondrial DNA; morphotype Strain(s): 837, 838, 1000, 1311, 1312, 1313, 1314 DOI: 10.1111/j.1529-8817.2011.01053.x Fukuda, S., Suzuki, I., Hama, T., Shiraiwa, Y. 2011 Compensatory response of the unicellular-calcifying alga Emiliania huxleyi (Coccolithophoridales, Haptophyta) to ocean acidification. J. Oceanogr., 67, 17-25. Keywords: Alkalization; Calcification; Coccolithophorid; Emiliania huxleyi; Inorganic carbon; Ocean acidiﬁcation; pH change; pH effect; Photosynthesis; Respriration Strain(s): 837 DOI: 10.1007/s10872-011-0001-z Araie, H., Sakamoto, K., Suzuki, I., Shiraiwa, Y. 2011 Characterization of the selenite uptake mechanism in the coccolithophore Emiliania huxleyi (Haptophyta). Plant Cell Physiol., 52, 1204-1210. Strain(s): 837 PubMed: 21632656 DOI: 10.1093/pcp/pcr070 Satoh, M., Iwamoto, K., Suzuki, I., Shiraiwa, Y. 2009 Cold stress stimulates intracellular calcification by the coccolithophore, Emiliania huxleyi (Haptophyceae) under phosphate-deficient conditions. Mar. Biotechnol., 11, 327-333. Keywords: calcification; coccolith production; coccolithophore; cold stress; Emiliania huxleyi; phosphate deprivation Strain(s): 837 PubMed: 18830665 DOI: 10.1007/s10126-008-9147-0 Ono, M., Sawada, K., Kubota, M., Shiraiwa, Y. 2009 Change of the unsaturation degree of alkenone and alkenoate during acclimation to salinity change in Emiliania huxleyi and Gephyrocapsa oceanica with reference to palaeosalinity indicator. Res. Org. Geochem., 25, 53-60. Strain(s): 837, 838 DOI: 10.20612/rog.25.0_53 Kayano, K. & Shiraiwa, Y. 2009 Physiological regulation of coccolith polysaccharide production by phosphate availability in the coccolithophorid Emiliania huxleyi. Plant Cell Physiol., 50, 1522-1531. Strain(s): 837 PubMed: 19587028 DOI: 10.1093/pcp/pcp097 Tsuji, Y., Suzuki, I., Shiraiwa, Y. 2009 Photosynthetic carbon assimilation in the coccolithophorid Emiliania huxleyi (Haptophyta): Evidence for the predominant Operation of the C ₃ cycle and the Contribution of β-carboxylases to the active anaplerotic reaction. Plant Cell Physiol., 50, 318-329. Strain(s): 837 PubMed: 19109302 DOI: 10.1093/pcp/pcn200 Shiraiwa, Y. & Kayano, K. 2007 Coccolith. In Development of New Functional Materials Inspired by Biomineralization , Ed. By Kato, T., CMC Publications, Tokyo, pp. 52-63. Strain(s): 837 Obata, T. & Shiraiwa, Y. 2005 A novel eukaryotic selenoprotein in the haptophyte alga Emiliania huxleyi. J. Biol. Chem., 280, 18462-18468. Strain(s): 837 DOI: 10.1074/jbc.M501517200 Sawada, K. & Shiraiwa, Y. 2004 Alkenone and alkenoic acid compositions of the membrane fractions of Emiliania huxleyi. Phytochemistry, 65, 1299-1307. Keywords: coccolithophorid; Emiliania huxleyi; Haptophyceae; alkenones; alkenoic acids; lipid biomarker; membrane fractionation; intracellular localization; unsaturation index Strain(s): 837 DOI: 10.1016/j.phytochem.2004.03.015 Shiraiwa, Y., Danbara, A., Kanke, Y. 2004 Characterization of highly oxygen-sensitive photosynthesis in coccolithophorids. Jpn. J. Phycol. (Sôrui), 52, Suppl., 87-94. Keywords: CO2 fixation; coccolithophorids; Emiliania huxleyi; glycolate pathway; O2 effect; O2 inhibition; photorespiratory nitrogen cycle; Warburg effect Strain(s): 837, 838 Obata, T., Sera, K., Futatsugawa, S., Shiraiwa, Y. 2004 Multi-element analysis of marine microalgae using particle-induced X-ray emission. Mar. Biotechnol., 6, 66-70. Keywords: coccolithophorid; element concentration; elemental composition; Emiliania huxleyi; multi-element analysis; particle-induced X-ray emmision Strain(s): 837 Obata, T., Araie, H., Shiraiwa, Y. 2004 Bioconcentration mechanism of selenium by a coccolithophorid, Emiliania huxleyi. Plant Cell Physiol., 45, 1434-1441. Keywords: coccolithophorid; Emiliania huxleyi; selenite uptake; selenium accumulation Strain(s): 837 PubMed: 15564527 DOI: 10.1093/pcp/pch164 Shiraiwa, Y. 2003 Physiological regulation of carbon fixation in the photosynthesis and calcification of coccolithophorids. Comp. Biochem. Physiol. B-Biochem. Mol. Biol., 136, 775-783. Keywords: carbon dioxide fixation; calcification; calcium carbonate crystal; cell growth; coccolith; coccolithophorids; Emiliania huxleyi; photosynthesis; nutrient-depletion effect; Warburg effect Strain(s): 837 PubMed: 14662302 DOI: 10.1016/S1096-4959(03)00221-5 Shiraiwa, Y., Hatano-Sugimoto, Y., Satoh, M. 2003 Regulation of intracellular calcification and algal growth by nutrient supply in coccolithophorids. In Biomineralization (BIOM2001): formation, diversity, evolution and application, Proceedings of th 8th International Symposium on Biomineralization, Eds. by Kobayashi & Ozawa, pp. 241-246. Keywords: algal bloom; calcification; coccolithophorid; Emiliania huxleyi; Gephyrocapsa oceanica; nitrate; phosphate; nutrient-depletion Strain(s): 837, 838 Iwamoto, K. & Shiraiwa, Y. 2003 Characterization of NADH nitrate reductase from the coccolithophorid Emiliania huxleyi (Lohman) Hay & Mohler (Haptophyceae). Mar. Biotechnol., 5, 20-26. Keywords: coccolithophorid; Emiliania huxleyi; enzyme purification; nitrate reductase; nitrate utilization Strain(s): 837 PubMed: 12925915 DOI: 10.1007/s10126-002-0051-8 Danbara, A. & Shiraiwa, Y. 1999 The requirement of selenium for the growth of marine coccolithophorids, Emiliania huxleyi, Gephyrocapsa oceanica and Helladosphaera sp. (Prymnesiophyceae). Plant Cell Physiol, 40, 762-766. Keywords: artificial seawater; coccolithophorids; Emiliania huxleyi; Gephyrocapsa oceanica; Helladosphaera; selenium-depletion; selenium-requirement Strain(s): 837, 838 DOI: 10.1093/oxfordjournals.pcp.a029603 Sawada, K., Handa, N., Shiraiwa, Y., Danbara, A., Montani, S. 1996 Long-chain alkenones and alkyl alkenoates in the coastal and pelagic sediments of the northwest North Pacific, with special reference to reconstruction of Emiliania huxleyi and Gephyrocapsa oceanica ratios. Org. Geochem., 24, 751-764. Keywords: alkenone, alkyl alkenoate; Uk'37; EE/K37; EE/K38; Emiliania huxleyi; Gephyrocapsa oceanica; coccolithophorid assemblage; northwest North Pacific sediment Strain(s): 837, 838 DOI: 10.1016/S0146-6380(96)00087-3 Sekino, K. & Shiraiwa, Y. 1996 Evidence for the involvement of mitochondrial respiration in calcification in a marine coccolithophorid, Emiliania huxleyi. Plant Cell Physiol., 37, 1030-1033. Keywords: calcification; CO2 fixation; coccolithophorids; Emiliania huxleyi; mitochondrial respiration; photosynthesis Strain(s): 837 DOI: 10.1093/oxfordjournals.pcp.a029034 Sekino, K., Kobayashi, H., Shiraiwa, Y. 1996 Role of coccoliths in the utilization of inorganic carbon by a marine unicellular coccolithophorid, Emiliania huxleyi: a survey using intact cells and protoplasts. Plant Cell Physiol., 37, 123-127. Keywords: calcification; coccolith; coccolithophorid; Emiliania huxleyi; inorganic carbon utilization; protoplast Strain(s): 837 DOI: 10.1093/oxfordjournals.pcp.a028921 Sekino, K. & Shiraiwa, Y. 1995 CO₂ release from the culture of calcifying and noncalcifying marine unicellular algae. J. Mar. Biotechnol., 3, 101-104. Strain(s): 837 Sekino, K. & Shiraiwa, Y. 1994 Accumulation and utilization of dissolved inorganic carbon by a marine unicellular coccolithophorid, Emiliania huxleyi. Plant Cell Physiol., 35, 353-361. Keywords: bicarbonate uptake; calcification; CO2 uptake; coccolithophorid; Emiliania huxleyi; inorganic carbon accumulation Strain(s): 837 DOI: 10.1093/oxfordjournals.pcp.a078603

Reference

Yoneda, K., Kobayashi, C., Araie, H., Morita, R., Harada, R., Shigeta, Y., Endo, H., Maeda, Y., Suzuki, I. 2025 Characterization of delta-7 alkenone desaturase in haptophyte Gephyrocapsa huxleyi through heterologous expression in Tisochrysis lutea. Mar. Biotechnol., 27, 44 (article ID).
Keywords: Gephyrocapsa huxleyi; Alkenones; Desaturase; Transformation; Heterologous expression; Tisochrysis lutea
Strain(s): 837
PubMed: 39921736
DOI: 10.1007/s10126-025-10427-y

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691

Pelusi, A., Hanawa, Y., Araie, H., Suzuki, I., Giordan, M., Shiraiwa, Y. 2016 Rapid detection and quantification of haptophyte alkenones by Fourier transform infrared spectroscopy (FTIR). Algal Res., 19, 48-56.
Keywords: Alkenones; Alkenone-producing haptophyte; Emiliania huxleyi; FTIR; Lipid;trans-Carbon-carbon double bond
Strain(s): 837
DOI: 10.1016/j.algal.2016.07.006

Nakamura, H., Sawada, K., Araie, H., Suzuki, I., Shiraiwa, Y. 2015 n-Nonacosadienes from the marine haptophytes Emiliania huxleyi and Gephyrocapsa oceanica. Phytochemistry, 111, 107-113.
Keywords: Emiliania huxleyi; Gephyrocapsa oceanica; Haptophyte; Nonacosadiene; Noelaerhabdaceae; Long chain alkene; Biomarker
Strain(s): 837, 1315
PubMed: 25595675
DOI: 10.1016/j.phytochem.2014.12.023

Tsuji, Y., Yamazaki, M., Suzuki, I., Shiraiwa, Y. 2015 Quantitative analysis of carbon flow into photosynthetic products functioning as carbon storage in the marine coccolithophore, Emiliania huxleyi. Mar. Biotechnol., 17, 428-440.
Keywords: Alkenones; Carbon partitioning; Carbon storage compound; Emiliania huxleyi; Haptophyte; Lipid; biosynthesis
Strain(s): 837
PubMed: 14730424
DOI: 10.1007/s10126-002-0081-2

Fukuda, S., Suzuki, Y., Shiraiwa, Y. 2014 Difference in physiological responses of growth, photosynthesis and calciﬁcation of the coccolithophore Emiliania huxleyi to acidiﬁcation by acid and CO₂ enrichment. Photosynth. Res., 121, 299-309.
Keywords: Acidiﬁcation; CO₂ enrichment; Calciﬁcation; Emiliania huxleyi; pH effect; Photosynthesis
Strain(s): 837
PubMed: 24500605
DOI: 10.1007/s11120-014-9976-9

Yamane, K., Matsuyama, S., Igarashi, K., Utsumi, M., Shiraiwa, Y., Kuwabara, T. 2013 Anaerobic coculture of microalgae with Thermosipho globiformans and Methanocaldococcus jannaschii at 68 ºC enhances generarion of n-alkane-rich biofules after pyrolysis. Appl. Environ. Microbiol., 79, 924-930.
Strain(s): 837, 2258
PubMed: 23183975
DOI: 10.1128/AEM.01685-12

Yamane, K., Matsuyama, S., Igarashi, K., Kuwabara, T. 2013 Pyrolytic Generation of Petroleum Crude Oils from the Marine Phytomicroalgal Coccolithophore Emiliania huxleyi (Haptophyta) and Preparation of n-Alkane-Rich Biofuel. Energy Fuels, 27, 7470–7479.
Strain(s): 837
DOI: 10.1021/ef401618t

Tsuji, Y., Suzuki, I., Shiraiwa, Y. 2012 Enzymological evidence for the function of a plastid-located pyruvate carboxylase in the Haptophyte alga Emiliania huxleyi: a novel pathway for the production of C ₄ compounds. Plant Cell Physiol., 53, 1043-1052.
Strain(s): 837
PubMed: 22492231
DOI: 10.1093/pcp/pcs045

Iwamoto, K. & Shiraiwa, Y. 2012 Characterization of intracellular iodine accumulation by iodine-tolerant microalgae. Procedia Environ. Sco., 15, 34-42.
Keywords: growth stimulation; growth inhibition; haptophyte; iodine; iodine accumulation
Strain(s): 377, 837, 838
DOI: 10.1016/j.proenv.2012.05.007

Mizoguchi, T., Kimura, Y., Yoshitomi, T., Tamiaki, H. 2011 The stereochemistry of chlorophyll-c₃ from the haptophyte Emiliania huxleyi: the (13²R)-enantiomers of chlorophylls-c are exclusively selected as the photosynthetically active pigments in chromophyte algae. Biochim. Biophys. Acta-Bioenerg., 1807, 1467-1473.
Keywords: chlorophyll-c; chiral HPLC; enantioimer; chromophyte algae; light-harvesting complexes
Strain(s): 71, 281, 696, 837
PubMed: 21806961
DOI: 10.1016/j.bbabio.2011.07.008

Kayano, K., Saruwatari, K., Kogure, T., Shiraiwa, Y. 2011 Effect of coccolith polysaccharides isolated from the coccolithophorid, Emiliania huxleyi, on calcite crystal formation in in vitro CaCO₃ crystallization. Mar. Biotechnol., 13, 83-92.
Keywords: Acid polysaccharide; Biomineralization; Coccolith; Coccolith polysaccharide; Calcium carbonate crystalization; Emiliania huxleyi
Strain(s): 837
PubMed: 20336339
DOI: 10.1007/s10126-010-9272-4

Hagino, K., Bendif, E. M., Young, J. R. Kogame, K., Probert, I., Takano, Y., Horiguchi, T., de Vardas, C., Okada, H. 2011 New evidence for morphological and genetic variation in the cosmopolitan coccolithophore Emiliania huxleyi (Prymnesiophyceae) from the COX1b-ATP4 genes. J. Phycol., 47, 1164-1176.
Keywords: biogeography; coccolithophore; Emiliania huxleyi; mitochondrial DNA; morphotype
Strain(s): 837, 838, 1000, 1311, 1312, 1313, 1314
DOI: 10.1111/j.1529-8817.2011.01053.x

Fukuda, S., Suzuki, I., Hama, T., Shiraiwa, Y. 2011 Compensatory response of the unicellular-calcifying alga Emiliania huxleyi (Coccolithophoridales, Haptophyta) to ocean acidification. J. Oceanogr., 67, 17-25.
Keywords: Alkalization; Calcification; Coccolithophorid; Emiliania huxleyi; Inorganic carbon; Ocean acidiﬁcation; pH change; pH effect; Photosynthesis; Respriration
Strain(s): 837
DOI: 10.1007/s10872-011-0001-z

Araie, H., Sakamoto, K., Suzuki, I., Shiraiwa, Y. 2011 Characterization of the selenite uptake mechanism in the coccolithophore Emiliania huxleyi (Haptophyta). Plant Cell Physiol., 52, 1204-1210.
Strain(s): 837
PubMed: 21632656
DOI: 10.1093/pcp/pcr070

Satoh, M., Iwamoto, K., Suzuki, I., Shiraiwa, Y. 2009 Cold stress stimulates intracellular calcification by the coccolithophore, Emiliania huxleyi (Haptophyceae) under phosphate-deficient conditions. Mar. Biotechnol., 11, 327-333.
Keywords: calcification; coccolith production; coccolithophore; cold stress; Emiliania huxleyi; phosphate deprivation
Strain(s): 837
PubMed: 18830665
DOI: 10.1007/s10126-008-9147-0

Ono, M., Sawada, K., Kubota, M., Shiraiwa, Y. 2009 Change of the unsaturation degree of alkenone and alkenoate during acclimation to salinity change in Emiliania huxleyi and Gephyrocapsa oceanica with reference to palaeosalinity indicator. Res. Org. Geochem., 25, 53-60.
Strain(s): 837, 838
DOI: 10.20612/rog.25.0_53

Kayano, K. & Shiraiwa, Y. 2009 Physiological regulation of coccolith polysaccharide production by phosphate availability in the coccolithophorid Emiliania huxleyi. Plant Cell Physiol., 50, 1522-1531.
Strain(s): 837
PubMed: 19587028
DOI: 10.1093/pcp/pcp097

Tsuji, Y., Suzuki, I., Shiraiwa, Y. 2009 Photosynthetic carbon assimilation in the coccolithophorid Emiliania huxleyi (Haptophyta): Evidence for the predominant Operation of the C ₃ cycle and the Contribution of β-carboxylases to the active anaplerotic reaction. Plant Cell Physiol., 50, 318-329.
Strain(s): 837
PubMed: 19109302
DOI: 10.1093/pcp/pcn200

Shiraiwa, Y. & Kayano, K. 2007 Coccolith. In Development of New Functional Materials Inspired by Biomineralization , Ed. By Kato, T., CMC Publications, Tokyo, pp. 52-63.
Strain(s): 837

Obata, T. & Shiraiwa, Y. 2005 A novel eukaryotic selenoprotein in the haptophyte alga Emiliania huxleyi. J. Biol. Chem., 280, 18462-18468.
Strain(s): 837
DOI: 10.1074/jbc.M501517200

Sawada, K. & Shiraiwa, Y. 2004 Alkenone and alkenoic acid compositions of the membrane fractions of Emiliania huxleyi. Phytochemistry, 65, 1299-1307.
Keywords: coccolithophorid; Emiliania huxleyi; Haptophyceae; alkenones; alkenoic acids; lipid biomarker; membrane fractionation; intracellular localization; unsaturation index
Strain(s): 837
DOI: 10.1016/j.phytochem.2004.03.015

Shiraiwa, Y., Danbara, A., Kanke, Y. 2004 Characterization of highly oxygen-sensitive photosynthesis in coccolithophorids. Jpn. J. Phycol. (Sôrui), 52, Suppl., 87-94.
Keywords: CO2 fixation; coccolithophorids; Emiliania huxleyi; glycolate pathway; O2 effect; O2 inhibition; photorespiratory nitrogen cycle; Warburg effect
Strain(s): 837, 838

Obata, T., Sera, K., Futatsugawa, S., Shiraiwa, Y. 2004 Multi-element analysis of marine microalgae using particle-induced X-ray emission. Mar. Biotechnol., 6, 66-70.
Keywords: coccolithophorid; element concentration; elemental composition; Emiliania huxleyi; multi-element analysis; particle-induced X-ray emmision
Strain(s): 837

Obata, T., Araie, H., Shiraiwa, Y. 2004 Bioconcentration mechanism of selenium by a coccolithophorid, Emiliania huxleyi. Plant Cell Physiol., 45, 1434-1441.
Keywords: coccolithophorid; Emiliania huxleyi; selenite uptake; selenium accumulation
Strain(s): 837
PubMed: 15564527
DOI: 10.1093/pcp/pch164

Shiraiwa, Y. 2003 Physiological regulation of carbon fixation in the photosynthesis and calcification of coccolithophorids. Comp. Biochem. Physiol. B-Biochem. Mol. Biol., 136, 775-783.
Keywords: carbon dioxide fixation; calcification; calcium carbonate crystal; cell growth; coccolith; coccolithophorids; Emiliania huxleyi; photosynthesis; nutrient-depletion effect; Warburg effect
Strain(s): 837
PubMed: 14662302
DOI: 10.1016/S1096-4959(03)00221-5

Shiraiwa, Y., Hatano-Sugimoto, Y., Satoh, M. 2003 Regulation of intracellular calcification and algal growth by nutrient supply in coccolithophorids. In Biomineralization (BIOM2001): formation, diversity, evolution and application, Proceedings of th 8th International Symposium on Biomineralization, Eds. by Kobayashi & Ozawa, pp. 241-246.
Keywords: algal bloom; calcification; coccolithophorid; Emiliania huxleyi; Gephyrocapsa oceanica; nitrate; phosphate; nutrient-depletion
Strain(s): 837, 838

Iwamoto, K. & Shiraiwa, Y. 2003 Characterization of NADH nitrate reductase from the coccolithophorid Emiliania huxleyi (Lohman) Hay & Mohler (Haptophyceae). Mar. Biotechnol., 5, 20-26.
Keywords: coccolithophorid; Emiliania huxleyi; enzyme purification; nitrate reductase; nitrate utilization
Strain(s): 837
PubMed: 12925915
DOI: 10.1007/s10126-002-0051-8

Danbara, A. & Shiraiwa, Y. 1999 The requirement of selenium for the growth of marine coccolithophorids, Emiliania huxleyi, Gephyrocapsa oceanica and Helladosphaera sp. (Prymnesiophyceae). Plant Cell Physiol, 40, 762-766.
Keywords: artificial seawater; coccolithophorids; Emiliania huxleyi; Gephyrocapsa oceanica; Helladosphaera; selenium-depletion; selenium-requirement
Strain(s): 837, 838
DOI: 10.1093/oxfordjournals.pcp.a029603

Sawada, K., Handa, N., Shiraiwa, Y., Danbara, A., Montani, S. 1996 Long-chain alkenones and alkyl alkenoates in the coastal and pelagic sediments of the northwest North Pacific, with special reference to reconstruction of Emiliania huxleyi and Gephyrocapsa oceanica ratios. Org. Geochem., 24, 751-764.
Keywords: alkenone, alkyl alkenoate; Uk'37; EE/K37; EE/K38; Emiliania huxleyi; Gephyrocapsa oceanica; coccolithophorid assemblage; northwest North Pacific sediment
Strain(s): 837, 838
DOI: 10.1016/S0146-6380(96)00087-3

Sekino, K. & Shiraiwa, Y. 1996 Evidence for the involvement of mitochondrial respiration in calcification in a marine coccolithophorid, Emiliania huxleyi. Plant Cell Physiol., 37, 1030-1033.
Keywords: calcification; CO2 fixation; coccolithophorids; Emiliania huxleyi; mitochondrial respiration; photosynthesis
Strain(s): 837
DOI: 10.1093/oxfordjournals.pcp.a029034

Sekino, K., Kobayashi, H., Shiraiwa, Y. 1996 Role of coccoliths in the utilization of inorganic carbon by a marine unicellular coccolithophorid, Emiliania huxleyi: a survey using intact cells and protoplasts. Plant Cell Physiol., 37, 123-127.
Keywords: calcification; coccolith; coccolithophorid; Emiliania huxleyi; inorganic carbon utilization; protoplast
Strain(s): 837
DOI: 10.1093/oxfordjournals.pcp.a028921

Sekino, K. & Shiraiwa, Y. 1995 CO₂ release from the culture of calcifying and noncalcifying marine unicellular algae. J. Mar. Biotechnol., 3, 101-104.
Strain(s): 837

Sekino, K. & Shiraiwa, Y. 1994 Accumulation and utilization of dissolved inorganic carbon by a marine unicellular coccolithophorid, Emiliania huxleyi. Plant Cell Physiol., 35, 353-361.
Keywords: bicarbonate uptake; calcification; CO2 uptake; coccolithophorid; Emiliania huxleyi; inorganic carbon accumulation
Strain(s): 837
DOI: 10.1093/oxfordjournals.pcp.a078603

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.