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Strain number NIES-836  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Botryococcus braunii Kützing  
Former name  
Common name Green alga  
Locality (Date of collection) Imuta-ike Pond, Kagoshima, Japan (1997-06-**)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Pond water)  
History < Mori, Fumi  
Isolator (Date of isolation) Mori, Fumi (1997-06-18)  
Identified by Mori, Fumi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2014 Feb]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  10-17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information 18S rRNA ( AB780364 JQ941954 ) , rbcL ( JQ941955 )  
Cell size (min - max) 7 - 11 μm  
Characteristics oil (hydrocarbon) production (Race B)  
Other strain no.  
Remarks Cryopreserved; Axenic; Very slow growth 
Maruyama, K. & Ohtsuki, T. 2024 A simple method for the preparation of single cells and regeneration of colonies of Botryococcus braunii NIES836. J. Microbiol. Methods, 216, 106859 (article ID).
Keywords: Botryococcus braunii; Colony regeneration; Microalgae; Nutrient limitation; Single-cell preparation
Strain(s): 836 
PubMed: 37995829
DOI: 10.1016/j.mimet.2023.106859

Guionet, A., Oura, K., Akiyama, H., Hosano, H. 2019 The narrow window of energy application for oil extraction by arc discharge. J. Appl. Phycol., 31, 89–96.
Keywords: Botryococcus braunii; Hydrocarbons; Biofuel; Matrix; Extraction
Strain(s): 836 
DOI: 10.1007/s10811-018-1579-y

Wan, M., Zhang, Z., Wang, R., Bai, W., Huang, J., Wang, W., Shen, G., Yu, A., Li, Y. 2019 High-yield cultivation of Botryococcus braunii for biomass and hydrocarbons. Biomass Bioenerg., 131, 105399 (article ID).
Keywords: Botryococcus braunii; Hydrocarbon; Heterotrophy; Photoautotrophy
Strain(s): 836 
DOI: 10.1016/j.biombioe.2019.105399

Guionet, A., Hosseini, B., Teissié, J., Akiyama, H., Hosseini, H. 2017 A new mechanism for efficient hydrocarbon electro-extraction from Botryococcus braunii. Biotechnol. Biofuels, 10, 39 (article ID).
Keywords: Green energy; Biofuel; Hydrocarbon; Extraction; Botryococcus braunii kützing; Nanosecond pulsed electric field (nsPEF)
Strain(s): 836 
PubMed: 28228843
DOI: 10.1186/s13068-017-0724-1

Okumura, C., Saffreena, N., Rahman, M. A., Hasegawa, H., Miki, O., Takimoto, A. 2015 Economic efficiency of different light wavelengths and intensities using LEDs for the cultivation of green microalga Botryococcus braunii (NIES-836) for biofuel production. Environ. Prog. Sustain. Energy, 34, 269-275.
Keywords: microalgae; Botryococcus braunii (NIES-836); growth efficiency; light-emitting diodes (LEDS); economic efficiency
Strain(s): 836 
DOI: 10.1002/ep.11951

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 1815116025529630636036364072283614091410141114191837216421993374 
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Kim, B.-H., Ramanan, R., Cho, D.-H., Choi, G.-G., La, H.-J., Ahn, C.-Y., Oh, H.-M., Kim, H.-S. 2012 Simple, rapid and cost-effective method for high quality nucleic acids extraction from different strains of Botryococcus braunii. PLoS One, 7, e37770 (article ID).
Strain(s): 836 
PubMed: 22662217
DOI: 10.1371/journal.pone.0037770

Ranga Rao, A., Ravishankar, G. A., Sarada, R. 2012 Cultivation of green alga Botryococcus braunii in raceway, circular ponds under outdoor conditions and its growth, hydrocarbon production. Bioresour. Technol., 123, 528-533.
Keywords: Microalgae; Botryococcus braunii; Outdoor cultivation; Hydrocarbon; Fatty acids
Strain(s): 836 
PubMed: 22940364
DOI: 10.1016/j.biortech.2012.07.009

Mogi, K., Fujii, T. 2012 Evaluation of ethanol toxicity to oil producing algae using a microfluidic device. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Okinawa, Japan 2012, pp. 857-859.
Keywords: Microalgae; Botryococcus braunii; Toxicity assay; Vernier wall
Strain(s): 836 

Kawahara, K., Sagehashi, M., Fujii, T., Fujita, H., Hu, H.-Y., Sakoda, A. 2011 Potential of a green alga Botryococcus braunii for simultaneous water purification and biofuel production under open-air condition. J. Water Environ. Technol., 9, 29-37.
Keywords: biofuel production; Botryococcus braunii; Microcystis aeruginosa; water purification
Strain(s): 878361090 
DOI: 10.2965/jwet.2011.29

Toyoda, K., Gishi, M., Ihara, I. 2011 Effect of light quality and nutrients on growth of hydrocarbon-rich microalgae, Botryococcus braunii. In ISHS Acta Horticulturae 907: VI International Symposium on Light in Horticulture, pp. 255-258.
Keywords: growth rate; phosphate; nitrogen; turbidity; ATR-FTIR
Strain(s): 836 

Li, Y., Qin, J. G. 2005 Comparison of growth and lipid content in three Botryococcus braunii strains. J. Appl. Phycol., 17, 551-556.
Keywords: temperature; irradiance; salinity; Botryococcus braunii; hydrocarbon
Strain(s): 836 
DOI: 10.1007/s10811-005-9005-7

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