A big image opens when the thumbnail is clicked.(nies-4017.jpg)
Strain number NIES-4017  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Astrephomene gubernaculifera Pocock  
Former name  
Common name Green alga  
Locality (Date of collection) Yokota, Sodegaura, Chiba, Japan (2014-07-21)  
Latitude / Longitude 35.389750 / 140.026722 
Habitat (Isolation source) Freshwater (Sediment)  
History < Yamashita, Shota; Nozaki, Hisayoshi  
Isolator (Date of isolation) Yamashita, Shota (2014-10-02)  
Identified by Yamashita, Shota  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  14 D  
Gene information Whole-genome ( BMAR00000000 ) , rbcL ( LC188997 )  
Cell size (min - max) 8 - 20 μm  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Heterotrophic ; Phototaxis ; Genome decoded strain (Yamashita et al. 2021) ; Heterothallic ; Isogamous ; Haploid life cycle ; Resting spore-forming  
Other strain no. Other strain no. : 2014-1002-YkAs8  
Remarks Cryopreserved 
Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamashita, S., Yamaguchi, H., Kawachi, K. 2023 Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene. BMC Microbiol., 23, 16 (article ID).
Keywords: Asexual cycle; Astrephomene; Cryopreservation; Cryoprotectant; Culture collection; Volvocine green algae
Strain(s): 4184195645656288538548554017 
PubMed: 36650459
DOI: 10.1186/s12866-023-02767-3

Yamashita, S., Yamamoto, K., Matsuzaki, R., Shigekatsu, S., Yamaguchi, H., Hirooka, S., Minakuchi, Y., Miyagishima, S., Masanobu, M., Atsushi, T., Hisayoshi, N. 2021 Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation. Sci Rep, 11, 22231 (article ID).
Strain(s): 4017 
PubMed: 34811380
DOI: 10.1038/s41598-021-01521-x

Yamashita, S., Arakaki, Y., Kawai-Toyooka, H., Noga, A., Hirono, M., Nozaki, H. 2016 Alternative evolution of a spheroidal colony in volvocine algae: developmental analysis of embryogenesis in Astrephomene (Volvocales, Chlorophyta). BMC Evol. Biol., 16, 243 (article ID).
Strain(s): 40174018 
PubMed: 27829356
DOI: 10.1186/s12862-016-0794-x

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