Strain Data

A big image opens when the thumbnail is clicked.(nies-4017.jpg)

Strain number		NIES-4017
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Astrephomene gubernaculifera Pocock
Synonym
Former name
Common name		Green alga
Locality (Date of collection)		Yokota, Sodegaura, Chiba, Japan (2014-07-21)
Latitude / Longitude		35.389750 / 140.026722
Habitat (Isolation source)		Freshwater (Sediment)
History		< Yamashita, Shota; Nozaki, Hisayoshi
Isolator (Date of isolation)		Yamashita, Shota (2014-10-02)
Identified by		Yamashita, Shota
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic[2018 Mar]
Culture condition (Preculture condition)		Medium: VTAC Temperature: 20 C Light intensity: 20 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 14 D
Gene information		Whole-genome ( BMAR00000000 ) , rbcL ( LC188997 )
Cell size (min - max)		8 - 20 μm
Organization		Colonial; Flagellate
Characteristics		Mixotrophic ; Heterotrophic ; Phototaxis ; Genome decoded strain (Yamashita et al. 2021) ; Heterothallic ; Isogamous ; Haploid life cycle ; Resting spore-forming
Other strain no.		Other strain no. : 2014-1002-YkAs8
Remarks		Cryopreserved
Movie

Reference
Chen, Y., Xiong, Q., Dai, Q., Liu, G. 2025 Six newly sequenced chloroplast genomes from quadriflagellate Chlamydomonadales (Chlorophyceae): Phylogeny and comparative genome analyses. Genome Biol. Evol., 17, evaf074 (article ID). Keywords: quadriflagellate chlamydomonadaleans; phylogenomics; Corbierea; Strain(s): 153, 257, 425, 432, 474, 566, 728, 875, 1363, 4017 PubMed: 40247659 DOI: 10.1093/gbe/evaf074 Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamashita, S., Yamaguchi, H., Kawachi, K. 2023 Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene. BMC Microbiol., 23, 16 (article ID). Keywords: Asexual cycle; Astrephomene; Cryopreservation; Cryoprotectant; Culture collection; Volvocine green algae Strain(s): 418, 419, 564, 565, 628, 853, 854, 855, 4017 PubMed: 36650459 DOI: 10.1186/s12866-023-02767-3 Yamashita, S., Yamamoto, K., Matsuzaki, R., Shigekatsu, S., Yamaguchi, H., Hirooka, S., Minakuchi, Y., Miyagishima, S., Masanobu, M., Atsushi, T., Hisayoshi, N. 2021 Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation. Sci Rep, 11, 22231 (article ID). Strain(s): 4017 PubMed: 34811380 DOI: 10.1038/s41598-021-01521-x Yamashita, S., Arakaki, Y., Kawai-Toyooka, H., Noga, A., Hirono, M., Nozaki, H. 2016 Alternative evolution of a spheroidal colony in volvocine algae: developmental analysis of embryogenesis in Astrephomene (Volvocales, Chlorophyta). BMC Evol. Biol., 16, 243 (article ID). Strain(s): 4017, 4018 PubMed: 27829356 DOI: 10.1186/s12862-016-0794-x

Reference

Chen, Y., Xiong, Q., Dai, Q., Liu, G. 2025 Six newly sequenced chloroplast genomes from quadriflagellate Chlamydomonadales (Chlorophyceae): Phylogeny and comparative genome analyses. Genome Biol. Evol., 17, evaf074 (article ID).
Keywords: quadriflagellate chlamydomonadaleans; phylogenomics; Corbierea; Strain(s): 153, 257, 425, 432, 474, 566, 728, 875, 1363, 4017
PubMed: 40247659
DOI: 10.1093/gbe/evaf074

Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamashita, S., Yamaguchi, H., Kawachi, K. 2023 Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene. BMC Microbiol., 23, 16 (article ID).
Keywords: Asexual cycle; Astrephomene; Cryopreservation; Cryoprotectant; Culture collection; Volvocine green algae
Strain(s): 418, 419, 564, 565, 628, 853, 854, 855, 4017
PubMed: 36650459
DOI: 10.1186/s12866-023-02767-3

Yamashita, S., Yamamoto, K., Matsuzaki, R., Shigekatsu, S., Yamaguchi, H., Hirooka, S., Minakuchi, Y., Miyagishima, S., Masanobu, M., Atsushi, T., Hisayoshi, N. 2021 Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation. Sci Rep, 11, 22231 (article ID).
Strain(s): 4017
PubMed: 34811380
DOI: 10.1038/s41598-021-01521-x

Yamashita, S., Arakaki, Y., Kawai-Toyooka, H., Noga, A., Hirono, M., Nozaki, H. 2016 Alternative evolution of a spheroidal colony in volvocine algae: developmental analysis of embryogenesis in Astrephomene (Volvocales, Chlorophyta). BMC Evol. Biol., 16, 243 (article ID).
Strain(s): 4017, 4018
PubMed: 27829356
DOI: 10.1186/s12862-016-0794-x

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