Strain number NIES-2677  
Phylum Cercozoa  
Class Chlorarachniophyceae  
Scientific name Bigelowiella natans Moestrup  
Synonym  
Former name  
Common name  
Locality (Date of collection) Mediterranean Sea (Depth -25m) (1999-09-30)  
Latitude / Longitude 43.4 / 7.81666666 
Habitat (Isolation source) Marine (Seawater)  
History < RCC (2010);  
Isolator (Date of isolation) Le Gall, F. (Re-isolation) < Partensky, F. (1999-09-30)  
Identified by Identified by DNA analysis at RCC  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  37 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( FJ973362 ) , 28S rRNA ( FJ973378 ) , ITS1-5.8S-ITS2 ( FJ821376 FJ821379 ) , actin ( FJ973395 ) , beta-tubulin ( FJ973423 )  
Cell size (min - max) - 16 μm  
Organization  
Characteristics Planktonic  
Other strain no. Other collection strain no. : RCC 337
Other strain no. : PROSOPE_153-1  
Remarks  
Movie  
Reference
Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62.
Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine
Strain(s): 1247482532742762772822863043313813874636236246997067157411443214521462149235123782433253525842588263526772716272828603576374540604109428043914392 

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 82542742863243313533814946236249971012101310151320133013331334133513721377138813901396139714081438143914411868196420082142214421492300230523252411243324982502256625842586258925902677287833563374360437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Gile, G. H., Stern, R. F., James, E. R., Keeling, P. J. 2010 DNA barcoding of chlorarachniophytes using nucleomorph its sequences. J. Phycol., 46, 743-750.
Keywords: Bigelowiella; Chlorarachnion; culture collections; Gymnochlora; internal transcribed spacer; Lotharella; Norrisiella; Partenskyella
Strain(s): 62425842677 
DOI: 10.1111/j.1529-8817.2010.00851.x

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