Strain number NIES-3374  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlorogonium capillatum Nozaki, M.M.Watanabe & Aizawa  
Former name  
Common name Green alga  
Locality (Date of collection) Osaka, Japan  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Pond water)  
History < Suzaki, Toshinobu  
Isolator (Date of isolation) Suzaki, Toshinobu (1988-**-**)  
Identified by Suzaki, Toshinobu  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  11-17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information act1 ( AB973441 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Planktonic ; Prey for protozoa (flagellates, ciliates, amoebids, heliozoans)  
Other strain no. Other strain no. : ATCC 50936  
Remarks Axenic 
Azuma, T., Pánek, T., Tice, A. K., Kayama, M., Kobayashi, M., Miyashita, H., Suzaki, T., Yabuki, A., Brown, M. W., Kamikawa, R. 2022 An enigmatic stramenopile sheds light on early evolution in Ochrophyta plastid organellogenesis. Mol. Biol. Evol., 39, msac065 (article ID).
Keywords: Actinophryidae; plastid evolution; aminoacyl-tRNA synthase; gene transfer; phylogenomics; organellar DNA
Strain(s): 24973374 
PubMed: 35348760
DOI: 10.1093/molbev/msac065

Hoshina, R., Tsukii, Y., Harumoto, T., Suzaki, T. 2021 Characterization of a green Stentor with symbiotic algae growing in an extremely oligotrophic environment and storing large amounts of starch granules in its cytoplasm. Sci Rep, 11, 2865 (article ID).
Strain(s): 3374 
PubMed: 33536497
DOI: 10.1038/s41598-021-82416-9

Higuchi, S., Sugahara, F., Pascual-Anaya, J., Takagi, W., Oisi, Y., Kuratani, S. 2019 Inner ear development in cyclostomes and evolution of the vertebrate semicircular canals. Nature, 565, 347–350.
Strain(s): 3374 
PubMed: 30518864
DOI: 10.1038/s41586-018-0782-y

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 82542742863243313533814946236249971012101310151320133013331334133513721377138813901396139714081438143914411868196420082142214421492300230523252433249825022566258425862589259026772878335633743775 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Higuchi, R., Song, C., Hoshina, R., Suzaki, T. 2018 Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea). Eur. J. Protistol., 66, 149-155.
Keywords: Cell wall; Chlorella variabilis; GlycosaminoglycansParamecium bursaria; Symbiosis
Strain(s): 3374 
PubMed: 30286318
DOI: 10.1016/j.ejop.2018.09.003

Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. 2015 Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. Plant Syst. Evol., 301, 1725-1733.
Keywords: Volvocales; Divergent actin
Strain(s): 3515465767173374 
DOI: 10.1007/s00606-014-1187-5

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 1815116025529630636036364072283614091410141114191837216421993374 
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Song, C., Suzaki, T. 2013 Improved preservation of organelies in Paramecium bursaria by freeze-substitution with glutaraldehyde and osmium tetroxide. J. Electr. Microsc. Technol. Med. Biol., 27, 1-8.
Keywords: Paramecium bursaria; endosymbiosis; cryofixation; ultrastructural preservation; freeze-substitution
Strain(s): 3374 

Sakaguchi, M., Suzuki, T., Khan, S. M. M. K. Hausmann, K. 2002 Food capture by kinetocysts in the heliozoon Raphidiophrys contractilis. Eur. J. Protistol., 37, 453-458.
Keywords: Exocytosis; Extrusome; Heliozoa; Kinetocyst
Strain(s): 24983374 
DOI: 10.1078/0932-4739-00847

Sakaguchi, M., Murakami, H., Suzaki, T. 2001 Involvement of a 40-kDa glycoprotein in food recognition, prey capture, and induction of phagocytosis in the protozoon Actinophrys sol. Protist, 152, 33-41.
Strain(s): 24973374 
PubMed: 11401035
DOI: 10.1078/1434-4610-00041

Sakaguchi, M., Suzuki, T. 1999 Monoxenic culture of the heliozoon Actinophrys sol. Eur. J. Protistol., 35, 1-10.
Keywords: Actinophrys sol; Antibiotic; Heliozoa; Monoxenic Culture
Strain(s): 24973374 
DOI: 10.1016/S0932-4739(99)80050-9

Kinoshita, E., Suzaki, T., Sugiyama, M., Shigenaka, Y. 1995 Ultrastructure and rapid axopodial contraction of a heliozoan, Raphidiophyrys contractilis sp. nov. J. Eucaryot. Microbiol., 42, 283-288.
Keywords: Contraction; Heliozoa; microtubule pattern; new species; Raphidiophyrys; silicic scale; X-ray microanalysis
Strain(s): 24983374 
DOI: 10.1111/j.1550-7408.1995.tb01581.x

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