Strain number NIES-898  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvulina steinii Playfair  
Former name  
Common name Green alga  
Locality (Date of collection) Wilson County, Texas, USA  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Soil or Mud)  
History < Nozaki, Hisayoshi < UTEX  
Isolator (Date of isolation) Carefoot, J. R.  
Identified by Carefoot, J. R.  
State of strain Subculture; Unialgal; Clonal; Non-axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  10-14 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information ITS1-5.8S-ITS2-28S ( HG422750 ) , atpB ( AB044175 ) , rbcL ( AB044161 ) , psaA ( AB044224 ) , psaB ( AB044450 ) , psbC ( AB044502 AB044503 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Phototaxis ; Heterothallic ; Isogamous  
Other strain no. Other collection strain no. : UTEX 1531
Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylogenet. Evol., 17, 256-268.
Strain(s): 418456460474541545564566569571572574577582653654666720721722724725727728730731732734735736737851854872882884886887888889890893895896898 
PubMed: 11083939
DOI: 10.1006/mpev.2000.0831

Nozaki, H., Hara, Y., Kasaki, H. 1987 Light and electron microscopy of pyrenoids and species delimitation in Volvulina (Chlorophyta, Volvocaceae). J. Phycol., 23, 359-364.
Keywords: Chlorophyta; culture age; pyrenoid; pyrenoid development; taxonomy; ultrastructure; Volvocaceae; Volvulina pringsheimii; Volvulina steinii
Strain(s): 545896897898 
DOI: 10.1111/j.1529-8817.1987.tb04145.x

Nozaki, H. 1982 Morphology and reproduction of Japanese Volvulina steinii (Chlorophyta, Volvocales). J. Jpn. Bot., 57, 105-113, pls. 3-4.
Strain(s): 545546896897898 

Carefoot, J. R. 1966 Sexual reproduction and intercrossing in Volvulina steinii. J. Phycol., 2, 150-156.
Strain(s): 896897898 
PubMed: 27053484
DOI: 10.1111/j.1529-8817.1966.tb04614.x

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