Strain number NIES-4399  
Phylum Haptophyta  
Class Coccolithophyceae  
Scientific name Braarudosphaera bigelowii (Gran & Braarud) Deflandre  
Synonym Pontosphaera bigelowii Gran & Braarud 1935; Chrysochromulina parkeae J.C.Green & Leadbeater 1972  
Former name  
Common name Coccolithophore  
Locality (Date of collection) Furue Bay, Furue-cho, Hirado, Nagasaki, Japan (2018-05-01)  
Latitude / Longitude 33.369520 / 129.518225 
Habitat (Isolation source) Marine (Seawater)  
History < Hagino, Kyoko  
Isolator (Date of isolation) Hagino, Kyoko (2018-05-03)  
Identified by Hagino, Kyoko  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  29 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  14 D  
Gene information Plastid DNA ( OR912953 OR912954 OR912955 )  
Cell size (min - max) 10 - 15 μm  
Organization Unicellular; Flagellate 
Characteristics Nitrogen-Fixing endosymbiont  
Other strain no. Other strain no. : FR-21  
Remarks Unstable; Fragile species to transportation stresses; Fragile species to temperature changes; Read and agree "How to order 4.1" 
Coale, T. H., Loconte, V., Turk-Kubo, K. A., Vanslembrouck, B., Mak, W. K. E., Cheung, S., Ekman, A., Chen, J.-H., Hagino, K., Takano, Y., Nishimura, T., Adachi, M., Le Gros, M., Larabell, C., Zehr, J. P. 2024 Nitrogen-fixing organelle in a marine alga. Science, 384,
Strain(s): 4399 
PubMed: 38603509
DOI: 10.1126/science.adk1075

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