Strain number NIES-3600  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Trichodesmium sp.  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Kuroshio current off Izu Peninsula, Japan (1983-10-13)  
Latitude / Longitude 34.66 / 138.41 
Habitat (Isolation source) Marine (Seawater)  
History < Ohki, Kaori  
Isolator (Date of isolation) Ohki, Kaori (1983-10-13)  
Identified by Ohki, Kaori  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  Modified AQUIL  
Temperature:  25 C
Light intensity:  8-11 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information nifH ( L00688 )  
Cell size (min - max)  
Organization Filamentous 
Characteristics Nitrogen fixation  
Other strain no. Other strain no. : NIBB1067  
Remarks Unstable; Fragile species to transportation stresses; Fragile species to temperature changes; Read and agree "How to order 4.1" 
Ohki, K. 2008 Intercellular localization of nitrogenase non-heterocystous Cyanobacterium (Cyanophyte), Trichodesmium sp. NIBB 1067. J. Oceanogr., 64, 211-216.
Keywords: Trichodesmium spp.; nitrogenase; whole-cell immunolocalization; immunocytochemical detection; in situ hybridization; diazotrophic cyanobacteria; horseradish peroxidaseconjugated probe; 3,3'-diaminobenzidine
Strain(s): 3600 

Taniuchi, Y., Murakami, A., Ohki, K. 2008 Whole-cell immunocytochemical detection of nitrogenase in cyanobacteria: improved protocol for highly fluorescent cells. Aquat. Microb. Ecol., 51, 237-247.
Keywords: Diazotrophic unicellular cyanobacteria; Marine cyanobacteria; Immunocytochemical detection; Nitrogenase; Unicellular cyanobacteria
Strain(s): 3600 
DOI: 10.3354/ame01197

Mulhollannd, M. R., Ohki, K., Capone, D. G. 2001 Nutrient controls on nitrogen uptake and metabolism by natural populations and cultures of Trichodesmium (Cyanobacteria). J. Phycol., 37, 1001-1009.
Keywords: Glutamate; glutamine; glutamine synthetase; N2 fixation; N uptake; Trichodesmium
Strain(s): 3600 
DOI: 10.1046/j.1529-8817.2001.00080.x

Mulhollannd, M. R., Ohki, K., Capone, D. G. 1999 Nitrogen utilization and metabolism relative to pattern of N2 fixation in cultures of Trichodesmium NIBB1067. J. Phycol., 35, 977-988.
Keywords: amino acids; glutamate; glutamine; glutamine synthetase; N turnover; N2 fixation; NH4+ uptake; NO3-; Trichodesmium; urea
Strain(s): 3600 
DOI: 10.1046/j.1529-8817.1999.3550977.x

Ohki, K. 1999 A possible role of temperate phage in the regulation of Trichodesmium biomass. In Marine cyanobacteria and related organisms, Eds. by Charpy, L., Larkum, Bulletin de l'Institut Oceanographique, Monaco, Special paper, 19, pp. 287-292.
Strain(s): 3600 

Mulholland, M.R., Shoemaker, C., Ohki, K., Capone, D.G. 1999 Utilization of combined forms of N in cultures and populations of Trichodesmium spp.. In Marine cyanobacteria and related organisms, Eds. by Charpy, L., Larkum, Bulletin de l'Institut Oceanographique, Monaco, Special paper, 19, pp. 279-286.
Strain(s): 3600 

Ohki, K., Kumazawa, S., Yoe, T-W., Ho, K-K. 1998 Improvement of artificial medium for the culture of marine microalgae and isolation and characterization of marine cyanobactria(Cyanophyta) from Singapore. In Asian Network on Microbial Researches, Eds. Gadjah Mada University, Riken and Science and Technology Agency, Japan 147-154.
Strain(s): 3600 

Ohki, K., Zehr, J. P., Fujita, Y. 1992 Regulation of nitrogenase activity in relation to the light-dark regime in the filamentous non-heterocystous cyanobacterium Trichodesmium sp. NIBB 1067. J. Gen. Microbiol., 138, 2679-2685.
Strain(s): 3600 
DOI: 10.1099/00221287-138-12-2679

Ohki, K. & Fujita, Y. 1992 Light-dependent maintenance of active nitrogenase in the non-heterocystous cyanophyte Trichodesmium sp. NIBB10687. In Research in photosynthesis, Ed. by Murata, N., Kluwer Academic Publishers, IV, pp. 103-106.
Strain(s): 3600 

Ohki, K., Zehr, J. P., Fujita, Y. 1992 Trichodesmium: Establishment of culture and characteristics of N2-fixation. In Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs, Eds. by Carpenter, E.J., Capone, D.G., Rueter, J.G., Springer, Dordrecht, 362, pp. 307-318.
Strain(s): 3600 
DOI: 10.1007/978-94-015-7977-3_20

Ohki, K., Zehr, J. P., Falkowsk, P. G., Fujita, Y. 1991 Regulation of nitrogen-fixation by different nitrogen sources in the marine non-heterocystous cyanobacterium Trichodesmium sp. NIBB1067. Arch. Microbiol., 156, 335-337.
Keywords: Cyanobacterium; Trichodesmium; Nitrogen-fixation; Nitrogenase
Strain(s): 3600 
DOI: 10.1007/BF00248706

Zehr, J. P., Ohki, K., Fujita, Y. 1991 Arrangement of nitrogenase structural genes in an aerobic filamentous nonheterocystous cyanobacterium. J. Bacteriol., 173, 7055-7058.
Strain(s): 3600 
PubMed: 1938909
DOI: 10.1128/jb.173.21.7055-7058.1991

Zehr, J. P., Ohki, K., Fujita, Y., Landry, D. 1991 Unique modification of adenine in genomic DNA of the marine cyanobacterium Trichodesmium sp. strain NIBB 1067. J. Bacteriol., 173, 7059-7062.
Strain(s): 3600 
PubMed: 1657876
DOI: 10.1128/jb.173.21.7059-7062.1991

Ohki, K., Falkowski, P.G., Rueter, J. G., Fujita, Y. 1991 Experimental study of the marine cyanophyte Trichodesmium sp., a nitrogen-fixing phytoplankton in tropical and subtropical sea area. In Marine biology: Its accomplishment and future prospect, Eds. by Mauchline, J., Nemoto, T., Hokusen-Sha, pp. 205-216.
Strain(s): 3600 

Rueter, J. G., Ohki, K., Fujita,Y. 1990 The effect of iron nutrition on photosynthesis and nitrogen fixation in cultures of Trichodesmium (cyanophyceae). J. Phycol., 26, 30-35.
Strain(s): 3600 
DOI: 10.1111/j.0022-3646.1990.00030.x

Ohki, K., Fujita, Y. 1988 Aerobic nitrogenase activity measured as acetylene reduction in the marine non-heterocystous cyanobacterium Trichodesmium spp. grown under artificial conditions. Mar. Biol., 98, 111-114.
Strain(s): 3600 
DOI: 10.1007/BF00392665

Ohki, K., Rueter, J. G., Fujita, Y. 1986 Cultures of the pelagic cyanophytes Trichodesmium erythraeum and T. thiebautii in synthetic medium. Mar. Biol., 91, 9-13.
Strain(s): 3600 
DOI: 10.1007/BF00397565

Ohki, K., Fujita, Y. 1982 Laboratory culture of the pelagic blue-green alga Trichodesmium thiebautii: conditions for unialgal culture. Mar. Ecol. Prog. Ser., 7, 185-190.
Strain(s): 3600 

Morel, F.M.M., Rueter, J.G., Anderson,D.M., Guillard, R.R. 1979 Aquil: a chemically defined phytoplankton culture medium for trace metal studies. J. Phycol., 15, 135-141.
Strain(s): 3600 
DOI: 10.1111/j.1529-8817.1979.tb02976.x

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