Strain number NIES-2666  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Former name  
Common name Desmid  
Locality (Date of collection) Kamigo Saitama, Japan (2004-08-08)  
Latitude / Longitude 36.2692 / 139.1331 
Habitat (Isolation source) Freshwater (Paddy water)  
History < Sekimoto, Hiroyuki  
Isolator (Date of isolation) Tsuchikane, Yuki (2004-08-10)  
Identified by Tsuchikane, Yuki  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  22 C
Light intensity:  11-14 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 5.8S-ITS2-28S ( AB625611 ) , 18S rRNA ( AB477426 ) , CpPI-kd01 ( AB558136 ) , CpPI-kd02 ( AB558137 ) , CpPI-kd03 ( AB558138 ) , CpPI-kd04 ( AB558139 ) , CpPI-kd05 ( AB558140 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Homothallic ; Isogamous  
Other strain no. Other strain no. : kodama20  
Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 5859606162636465676869702612666 
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Tsuchikane, Y., Kokubun, Y., Sekimoto, H. 2010 Characterization and molecular cloning of conjugation-regulating sex pheromones in homothallic Closterium. Plant Cell Physiol., 51, 1515-1523.
Keywords: Closterium; Conjugation; Homothallic; Homothallism; Sex pheromone; Sexual reproduction; Zygospore
Strain(s): 2666 
PubMed: 20656896
DOI: 10.1093/pcp/pcq110

Tsuchikane, Y., Sato, M., Ootaki, T., Kokubun, Y., Nozaki, H., Ito, M., Sekimoto, H. 2010 Sexual prosesses and phylogenetic relationship of a homothallic strain in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 46, 278-284.
Keywords: Closterium; conjugation; heterothallic; pheromone; sexual reproduction
Strain(s): 2666 
DOI: 10.1111/j.1529-8817.2010.00806.

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