Strain number NIES-2463  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas reinhardtii P.A.Dangeard  
Synonym Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
Former name  
Common name Green alga  
Locality (Date of collection) Kokubumuke-cho, Kirishima, Kagoshima, Japan (2006-03-26)  
Latitude / Longitude 31.75 / 130.76666666 
Habitat (Isolation source) Freshwater (Soil)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2008-11-27)  
Identified by Nakada, Takashi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  7-13 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB701555 AB511836 ) , ITS1-5.8S-ITS2 ( AB511842 ) , rbcL ( AB511846 ) , FUS1 ( AB900980 ) , NIT1 ( AB900988 ) , NIT2 ( AB900996 )  
Cell size (min - max) 8 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Heterothallic ; Dioecious ; Isogamous ; Mating type (+)  
Other strain no. Other strain no. : KkS0801B1  
Remarks Cryopreserved; Axenic 
Nitta, N. et al. 2020 Raman image-activated cell sorting. Nat. Commun., 11, 3452 (article ID).
Strain(s): 48229324633640 
PubMed: 32651381
DOI: 10.1038/s41467-020-17285-3

Lee, S., Thio, S. K., Park, S.-Y., Bae, S. 2019 An automated 3D-printed smartphone platform integrated withoptoelectrowetting (OEW) microfluidic chip for on-site monitoring of viablealgae in water. Harmful Algae, 88, 101638 (article ID).
Keywords: Algae quantification; In-situ assessment; Viable algae; 3D-printed smartphone platform; Optoelectorwetting
Strain(s): 8432463 
PubMed: 31582154
DOI: 10.1016/j.hal.2019.101638

Mao, F., He, Y., Gin, KY. 2018 Evaluating the joint toxicity of two benzophenone-type UV filters on the Ggreen alga Chlamydomonas reinhardtii with response surface methodology. Toxics, 6, 8 (article ID).
Keywords: Chlamydomonas reinhardtii; benzophenone-1; benzophenone-3; joint toxicity; response surface methodology
Strain(s): 2463 
PubMed: 29320457
DOI: 10.3390/toxics6010008

Mao, F., He, Y., Kushmaro, A., Gin, KY. 2017 Effects of benzophenone-3 on the green alga Chlamydomonas reinhardtii and the cyanobacterium Microcystis aeruginosa. Aquat. Toxicol., 193, 1-8.
Keywords: Benzophenone-3; Toxicity; Green algae; Cyanobacteria; Photosynthesis; Uptake
Strain(s): 8432463 
PubMed: 28992446
DOI: 10.1016/j.aquatox.2017.09.029

Nakada, T., Tsuchida, Y., Arakawa, K., Ito, T., Tomita, M. 2014 Hybridization between Japanese and North American Chlamydomonas reinhardtii (Volvocales, Chlorophyceae). Phycol. Res., 62, 232-236.
Keywords: biogeography; Chlamydomonas reinhardtii; Chlorophyceae; mating population; species
Strain(s): 246324643378337933803381 
DOI: 10.1111/pre.12061

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Nakada, T., Shinkawa, H., Ito, T., Tomita, M. 2010 Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan. J. Plant Res., 123, 67-78.
Keywords: Chlamydomonas reinhardtii; Chlorophyceae; distribution; phycology; species; taxonomy
Strain(s): 17081858246224632464 
PubMed: 19882207
DOI: 10.1007/s10265-009-0266-0

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