| Strain number | NIES-4118 | |||
|---|---|---|---|---|
| Phylum | Dinophyta | |||
| Class | Dinophyceae | |||
| Scientific name | Gambierdiscus scabrosus T.Nishimura, Shinya Sato & M.Adachi | |||
| Synonym | ||||
| Former name | ||||
| Common name | Dinoflagellate | |||
| Locality (Date of collection) | Ushiro Beach, Kashiwa Isl., Otsuki, Kochi, Japan (2007-09-22) | |||
| Latitude / Longitude | 32.772778 / 132.625278 | |||
| Habitat (Isolation source) | Marine (Seaweed) | |||
| History | < Nakamura, Masatoshi; Adachi, Masao | |||
| Isolator (Date of isolation) | Nakamura, Masatoshi (2007-09-**) | |||
| Identified by | Nishimura, Tomohiro; Sato, Shinya; Adachi, Masao | |||
| State of strain | Subculture; Unialgal; Clonal; Non-axenic | |||
|
Culture condition (Preculture condition) |
Medium:
MNK
Temperature: 20 C Light intensity: 6 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 45 D |
|||
| Gene information | 18S rRNA ( AB764244 AB764245 AB764246 AB764247 AB764248 ) , 28S rRNA ( AB765908 AB765909 ) | |||
| Cell size (min - max) | 49 - 83 μm | |||
| Organization | Unicellular | |||
| Characteristics | Toxicity ; Attached ; Authentic strain (Nishimura et al. 2014) | |||
| Other strain no. | Other strain no. : KW070922_1 | |||
| Remarks | Toxicity | |||
| Movie | ||||
| Reference |
|---|
|
Pisapia, F., Holland, W. C., Hardison, D. R., e Litake, R. W., Fraga, S., Nishimura, T., Adachi, M., Nguyen-Ngoc, L., Séchet, V., Amzil, Z., Herrenknecht, C., Hess, P.
2017
Toxicity screening of 13 Gambierdiscus strains using neuro-2a and erythrocyte lysis bioassays.
Harmful Algae,
63,
173-183.
Keywords: Ciguatera fish poisoning; Gambierdiscus; Ciguatoxins; Maitotoxins; Neuro-2a assay; Erythrocyte lysis assay Strain(s): 4118 PubMed: 28366392 DOI: 10.1016/j.hal.2017.02.005 Pisapia, F., Sibat, .M., Herrenknecht, C., Lhaut, K., Gaiani, G., Ferron, P., Fessard, V., Fraga, S., Nascimento, S. M., Litaker, R. W., Holland, W. C., Roullier, C., Hess, P. 2017 Maitotoxin-4, a novel MTX analog produced by Gambierdiscus excentricus. Mar. Drugs, 15, 220 (article ID). Keywords: Gambierdiscus excentricus; maitotoxin-4; bioguided fractionation; size-exclusion chromatography (LH-20); neuro-2a (N2a) assay; LC-HRMS/MS (Q-Tof 6550); LC-LRMS/MS (API4000 QTrap) Strain(s): 4118, 4119 PubMed: 28696398 DOI: 10.3390/md15070220 Nishimura, T., Hariganeya, N., Tawong, W., Sakanari, H., Yamaguchi, H., Adachi, M. 2016 Quantitative PCR assay for detection and enumeration of ciguatera-causing dinoflagellate Gambierdiscus spp. (Gonyaulacales) in coastal areas of Japan. Harmful Algae, 52, 11-22. Keywords: Ciguatera fish poisoning; Dinoflagellate; Gambierdiscus; Japan: Quantitative PCR assay Strain(s): 4118, 4119, 4120 PubMed: 28073467 DOI: 10.1016/j.hal.2015.11.018 Yoshimatsu, T., Tie, C., Yamaguchi, H., Funaki, H., Honma, C., Tanaka, K., Adachi, M. 2016 The effects of light intensity on the growth of Japanese Gambierdiscus spp. (Dinophyceae). Harmful Algae, 60, 107-115. Keywords: Ciguatera; Gambierdiscus; Growth; Ligh Strain(s): 4118, 4119, 4120 PubMed: 28073553 DOI: 10.1016/j.hal.2016.10.009 Nishimura, T., Sato, S., Tawong, W., Sakanari, H., Yamaguchi, H., Adachi, M. 2014 Morphology of Gambierdiscus scabrosus sp. nov. (Gonyaulacales): a new epiphytic toxic dinoflagellate from coastal areas of Japan. J. Phycol., 50, 506-514. Keywords: ciguatera fish poisoning; epiphytic; dinoflagellate; Gambierdiscus scabrosus; Japan; LM; morphology; SEM; taxonomy Strain(s): 4118 PubMed: 26988323 DOI: 10.1111/jpy.12175 Yoshimatsu, T., Yamaguchi, H., Iwamoto, H., Nishimura, T., Adachi, M. 2014 Effects of temperature, salinity and their interaction on growth of Japanese Gambierdiscus spp. (Dinophyceae). Harmful Algae, 35, 29-37. Keywords: Ciguatera; Japanese Gambierdiscus; Growth; Temperature; Salinity Strain(s): 4118, 4119, 4120 DOI: 10.1016/j.hal.2014.03.007 Hariganeya, N., Tanimoto, Y., Yamaguchi, H., Nishimura, T., Tawong, W., Sakanari, H., Yoshimatsu, T., Sato, S., Preston, C. M., Adachi, M. 2013 Quantitative PCR method for enumeration of cells of cryptic species of the toxic marine dinoflagellate Ostreopsis spp. in Coastal Waters of Japan. PLoS One, 8, e57627 (article ID). Strain(s): 4118 PubMed: 23593102 DOI: 10.1371/journal.pone.0057627 Nishimura, T., Sato, S., Tawong, W., Sakanari, H., Uehara, K., Shah, M.M., Suda, S., Yasumoto, T., Taira, Y., Yamaguchi, H., Adachi, M. 2013 Genetic diversity and distribution of the ciguatera-causing dinoflagellate Gambierdiscus spp. (Dinophyceae) in coastal areas of Japan. PLoS One, 8, e60882 (article ID). Strain(s): 4118, 4119, 4120 PubMed: 23593339 DOI: 10.1371/journal.pone.0060882 |
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