Strain number NIES-4271  
Phylum Dinophyta  
Class Dinophyceae  
Scientific name Breviolum sp.  
Synonym  
Former name  
Common name Dinoflagellate ; Zooxanthellae  
Locality (Date of collection) Coconut Isl., Hawaii, USA (wild type locality)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Symbiodinium strain SSB01)  
History < Maruyama, Shinichiro < Ishii, Yuu  
Isolator (Date of isolation) Ishii, Yuu (2016-07-26)  
Identified by Maruyama, Shinichiro  
State of strain Subculture; Unialgal; Clonal; Axenic[2020 Feb]  
Culture condition
(Preculture condition)
Medium:  MB  
Temperature:  24 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  45 D  
Gene information Ura3 ( LC363940 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Symbiotic ; Mixotrophic ; Osmotrophic ; Mutant of Symbiodinium strain SSB01 (Uracil-required;( 5-FOA-resistant tolerant)(Ishii et al. 2018)  
Other strain no. Other strain no. : T01  
Remarks Axenic 
Movie  
Reference
Ishii, Y., Maruyama, S., Fujimura-Kamada, K., Kutsuna, N., Takahashi, S., Kawata, M., Minagawa, J. 2018 Isolation of uracil auxotroph mutants of coral symbiont alga for symbiosis studies. Sci Rep, 8, 3237 (article ID).
Strain(s): 4271 
PubMed: 29459692
DOI: 10.1038/s41598-018-21499-3

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