Strain Data

Strain number		NIES-2238
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Chlamydomonas reinhardtii P.A.Dangeard
Synonym		Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941
Former name
Common name		Green alga
Locality (Date of collection)		near Amherst, Massachusetts, USA
Latitude / Longitude		42.364125 /
Habitat (Isolation source)		Terrestrial (Soil)
History		< IAM (2007) < Kuchitsu, Kazuyuki (1991) < Mihara, S. < Matsuda, Y. < Togasaki, R.
Isolator (Date of isolation)		Smith, G. M. (1945--)
Identified by
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: TAP (agar) Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M
Gene information		18S rRNA ( AB511835 AB701553 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Phototaxis ; Mating type (+) ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005)
Other strain no.		Other collection strain no. : IAM C-541; CCAP 11/32C; SAG 34.89; UTEX 2244; UTCC 11; CGC CC-125 mt⁺ Other strain no. : 137c mt⁺
Remarks		Cryopreserved; Axenic
Movie

Reference
Karitani, Y., Yamada, R., MatsumotoT., Ogino, H. 2024 UV mutagenesis improves growth potential of green algae in a green algae–yeast co‑culture system. Arch. Microbiol., 206, 61 (article ID). Keywords: Chlamydomonas reinhardtii; Co-cultivation; Photosynthesis; Saccharomyces cerevisiae; Mutagenesis; Transcriptome analysis Strain(s): 2238 PubMed: 38216809 DOI: 10.1007/s00203-023-03796-2 Karitani, Y., Yamada, R., Matsumoto, T., Ogino, H. 2024 Improvement of cell growth in green algae Chlamydomonas reinhardtii through co-cultivation with yeast Saccharomyces cerevisiae. Biotechnol. Lett., 46, 431-441. Keywords: Chlamydomonas reinhardtii; Co-cultivation; Photosynthesis; Saccharomyces cerevisiae; Transcriptome analysis Strain(s): 2238 PubMed: 38578514 DOI: 10.1007/s10529-024-03483-2 Shimizu, I., Yamashita, K., Tokunaga, E. 2022 Development of a simple fabrication method for magnetic micro stir bars and induction of rotational motion in Chlamydomonas reinhardtii. Micromachines, 13, 1842 (article ID). Keywords: magnetic micro stirrer bar; microchip; motion analysis; microalga; Chlamydomonas; neodymium magnet; flagella; orbital motion Strain(s): 2238 PubMed: 36363863 DOI: 10.3390/mi13111842 Yamada, R., Yokota, M., Matsumoto, T., Hankamer, B., Ogino, H. 2022 Promoting cell growth and characterizing partial symbiotic relationships in the co-cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli. Biotechnol. J., 18, e2200099 (article ID). Keywords: Chlamydomonas reinhardtii; co-culture; Escherichia coli; photosynthesis; symbiotic relationship Strain(s): 2238 PubMed: 36479591 DOI: 10.1002/biot.202200099 Toyoshima, H., Miyata, A., Yoshida, R., Ishige, T., Takaichi, S., Kawasaki, S. 2021 Distribution of the water-soluble astaxanthin binding carotenoprotein (AstaP) in Scenedesmaceae. Mar. Drugs, 19, 349 (article ID). Keywords: astaxanthin; Scenedesmus; Coelastrella; carotenoprotein; astaxanthin-binding protein Strain(s): 2238, 2540 PubMed: 34203079 DOI: 10.3390/md19060349 Yamashita, K., Tokunaga, E. 2020 Noninvasive and safe cell viability assay for Paramecium using natural pigment extracted from food. Sci Rep, 10, 10996 (article ID). Strain(s): 2238 PubMed: 32620770 DOI: 10.1038/s41598-020-67712-0 Isono, T., Yamashita, K., Momose, D., Kobayashi, H., Kitamura, M., Nishiyama, Y., Hosoya, T., Kanda, H., Kudo, A., Okada, N., Yagi, T., Nakata, K., Mineki, S., Tokunaga, E. 2015 Scan-free absorbance spectral imaging A(x, y, λ) of single live algal cells for quantifying absorbance of cell suspensions. PLoS One, 10, e0128002 (article ID). Strain(s): 2238 PubMed: 26061268 DOI: 10.1371/journal.pone.0128002 Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12. Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284 Pröschold, T., Harris, E. H., Coleman, A. W. 2005 Portrait of a species: Chlamydomonas reinhardtii. Genetics, 170, 1601-1610. Keywords: Chlamydomonas; Internal Transcribed Spacer; phylogenetics; population genetics; ribosomal cistrons Strain(s): 2237, 2238 PubMed: 15956662 DOI: 10.1534/genetics.105.044503 Matsuda, Y., Shimada, T., Sakamoto, Y. 1992 Ammonium ions control gametic differentiation and dedifferentiation in Chlamydomonas reinhardtii. Plant Cell Physiol., 33, 909-914. Keywords: cell cycle progression; Chlamydomonas reinhardtii; NH4+ starvation; organic nitrogen compounds; sexual reproduction Strain(s): 2238 DOI: 10.1093/oxfordjournals.pcp.a078340 Kimpel, D. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii I. Localization. Plant Cell Physiol., 24, 255-259. Keywords: carbonic anhydrase; cell wall-less mutant; Chyamydomonas reinhardtii; periplasm; wall lysing enzyme Strain(s): 2237, 2238 DOI: 10.1093/pcp/24.2.255 Spencer, K. G., Kimpel, D. L., Fisher, M. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii II. Requirements for carbonic anhydrase induction. Plant Cell Physiol., 24, 301-304. Keywords: carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; photosynthesis; photosynthetic mutants Strain(s): 2238 DOI: 10.1093/pcp/24.2.301 Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565. Strain(s): 2138, 2139, 2212, 2238, 2279

Reference

Karitani, Y., Yamada, R., MatsumotoT., Ogino, H. 2024 UV mutagenesis improves growth potential of green algae in a green algae–yeast co‑culture system. Arch. Microbiol., 206, 61 (article ID).
Keywords: Chlamydomonas reinhardtii; Co-cultivation; Photosynthesis; Saccharomyces cerevisiae; Mutagenesis; Transcriptome analysis
Strain(s): 2238
PubMed: 38216809
DOI: 10.1007/s00203-023-03796-2

Karitani, Y., Yamada, R., Matsumoto, T., Ogino, H. 2024 Improvement of cell growth in green algae Chlamydomonas reinhardtii through co-cultivation with yeast Saccharomyces cerevisiae. Biotechnol. Lett., 46, 431-441.
Keywords: Chlamydomonas reinhardtii; Co-cultivation; Photosynthesis; Saccharomyces cerevisiae; Transcriptome analysis
Strain(s): 2238
PubMed: 38578514
DOI: 10.1007/s10529-024-03483-2

Shimizu, I., Yamashita, K., Tokunaga, E. 2022 Development of a simple fabrication method for magnetic micro stir bars and induction of rotational motion in Chlamydomonas reinhardtii. Micromachines, 13, 1842 (article ID).
Keywords: magnetic micro stirrer bar; microchip; motion analysis; microalga; Chlamydomonas; neodymium magnet; flagella; orbital motion
Strain(s): 2238
PubMed: 36363863
DOI: 10.3390/mi13111842

Yamada, R., Yokota, M., Matsumoto, T., Hankamer, B., Ogino, H. 2022 Promoting cell growth and characterizing partial symbiotic relationships in the co-cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli. Biotechnol. J., 18, e2200099 (article ID).
Keywords: Chlamydomonas reinhardtii; co-culture; Escherichia coli; photosynthesis; symbiotic relationship
Strain(s): 2238
PubMed: 36479591
DOI: 10.1002/biot.202200099

Toyoshima, H., Miyata, A., Yoshida, R., Ishige, T., Takaichi, S., Kawasaki, S. 2021 Distribution of the water-soluble astaxanthin binding carotenoprotein (AstaP) in Scenedesmaceae. Mar. Drugs, 19, 349 (article ID).
Keywords: astaxanthin; Scenedesmus; Coelastrella; carotenoprotein; astaxanthin-binding protein
Strain(s): 2238, 2540
PubMed: 34203079
DOI: 10.3390/md19060349

Yamashita, K., Tokunaga, E. 2020 Noninvasive and safe cell viability assay for Paramecium using natural pigment extracted from food. Sci Rep, 10, 10996 (article ID).
Strain(s): 2238
PubMed: 32620770
DOI: 10.1038/s41598-020-67712-0

Isono, T., Yamashita, K., Momose, D., Kobayashi, H., Kitamura, M., Nishiyama, Y., Hosoya, T., Kanda, H., Kudo, A., Okada, N., Yagi, T., Nakata, K., Mineki, S., Tokunaga, E. 2015 Scan-free absorbance spectral imaging A(x, y, λ) of single live algal cells for quantifying absorbance of cell suspensions. PLoS One, 10, e0128002 (article ID).
Strain(s): 2238
PubMed: 26061268
DOI: 10.1371/journal.pone.0128002

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284

Pröschold, T., Harris, E. H., Coleman, A. W. 2005 Portrait of a species: Chlamydomonas reinhardtii. Genetics, 170, 1601-1610.
Keywords: Chlamydomonas; Internal Transcribed Spacer; phylogenetics; population genetics; ribosomal cistrons
Strain(s): 2237, 2238
PubMed: 15956662
DOI: 10.1534/genetics.105.044503

Matsuda, Y., Shimada, T., Sakamoto, Y. 1992 Ammonium ions control gametic differentiation and dedifferentiation in Chlamydomonas reinhardtii. Plant Cell Physiol., 33, 909-914.
Keywords: cell cycle progression; Chlamydomonas reinhardtii; NH4+ starvation; organic nitrogen compounds; sexual reproduction
Strain(s): 2238
DOI: 10.1093/oxfordjournals.pcp.a078340

Kimpel, D. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii I. Localization. Plant Cell Physiol., 24, 255-259.
Keywords: carbonic anhydrase; cell wall-less mutant; Chyamydomonas reinhardtii; periplasm; wall lysing enzyme
Strain(s): 2237, 2238
DOI: 10.1093/pcp/24.2.255

Spencer, K. G., Kimpel, D. L., Fisher, M. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii II. Requirements for carbonic anhydrase induction. Plant Cell Physiol., 24, 301-304.
Keywords: carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; photosynthesis; photosynthetic mutants
Strain(s): 2238
DOI: 10.1093/pcp/24.2.301

Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565.
Strain(s): 2138, 2139, 2212, 2238, 2279

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