| Strain number | NIES-2237 | |||
|---|---|---|---|---|
| Phylum | Chlorophyta | |||
| Class | Chlorophyceae | |||
| Scientific name | Chlamydomonas reinhardtii P.A.Dangeard | |||
| Synonym | Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941 | |||
| Former name | ||||
| Common name | Green alga | |||
| Locality (Date of collection) | near Amherst, Massachusetts, USA (locality of wild type strain (NIES-2238)) | |||
| Latitude / Longitude | ||||
| Habitat (Isolation source) | ||||
| History | < IAM (2007) < Mihara, S. (1991) | |||
| Isolator (Date of isolation) | ||||
| Identified by | ||||
| State of strain | Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb] | |||
|
Culture condition (Preculture condition) |
Medium:
TAP (agar)
Temperature: 20 C Light intensity: 2-3 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 6 M |
|||
| Gene information | 18S rRNA ( AB701552 ) | |||
| Cell size (min - max) | 5 - 18 μm | |||
| Organization | Unicellular; Flagellate | |||
| Characteristics | Phototaxis ; D-Threonine aldolase (Hirato et al. 2007) ; D-amino acids ; Cell wall less mutant of NIES-2238 ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005) | |||
| Other strain no. |
Other collection strain no. : IAM C-540
Other strain no. : cw-15 strain |
|||
| Remarks | Cryopreserved; Axenic | |||
| Movie | ||||
| Reference |
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Marchetto, F., Santaeufemia, S., Lebiedzińska-Arciszewska, M., Śliwińska, M. A., Pich, M., Kurek, E., Nazieblo, A., Strawski, M., Solymosi, D., Szklarczyk, M., Bulska, E., Szymański, J., Wierzbicka, M., Allahverdiyeva, Y., Wieckowski, M. R., Kargul, J.
2024
Dynamic adaptation of the extremophilic red microalga Cyanidioschyzon merolae to high nickel stress.
Plant Physiol. Biochem.,
207,
108365 (article ID).
Keywords: Abiotic stress; Adaptation; Cyanidioschyzon merolae; Heavy metals; Nickel; Photosynthesis and respiration Strain(s): 2237, 3377 PubMed: 38266563 DOI: 10.1016/j.plaphy.2024.108365 Tokunaga, S., Morimoto, D., Koyama, T., Kubo, Y., Shiroi, M., Ohara, K., Tokuhiro, H., Mori, Y., Nakagawa, S., Sawayama, S. 2021 Enhanced lutein production in Chlamydomonas reinhardtii by overexpression of the lycopene epsilon cyclase gene. Appl. Biochem. Biotechnol., 193, 1967–1978. Keywords: Lutein; Lycopene epsilon cyclase; Carotenoid; Chlamydomonas reinhardtii; Transgene Strain(s): 2237 PubMed: 33528746 DOI: 10.1007/s12010-021-03524-w Hirato, Y., Goto, M., Tokuhisa, M., Tanigawa, M., Nishimura, K. 2017 Crystallization and X-ray analysis of D-threonine aldolase from Chlamydomonas reinhardtii. Acta Crystallogr. F-Struct. Biol., F73, 86-89. Keywords: D-threonine aldolase; Chlamydomonas reinhardtii; D-amino acids; crystallization Strain(s): 2237 PubMed: 28177318 DOI: 10.1107/S2053230X1602063X Hirato, Y., Tokuhisa, M., Tanigawa, M., Ashida, H., Tanaka, H., Nishimura, K. 2017 Cloning and characterization of d-threonine aldolase from the green alga Chlamydomonas reinhardtii. Phytochemistry, 135, 18-23. Keywords: Chlamydomonas reinhardtii; Chlamydomonadaceae; Green alga; Enzyme characterization; d-Amino acid; d-Threonine aldolase; Pyridoxal 5’-phosphate Strain(s): 2237 PubMed: 28038776 DOI: 10.1016/j.phytochem.2016.12.012 Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12. Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284 Pröschold, T., Harris, E. H., Coleman, A. W. 2005 Portrait of a species: Chlamydomonas reinhardtii. Genetics, 170, 1601-1610. Keywords: Chlamydomonas; Internal Transcribed Spacer; phylogenetics; population genetics; ribosomal cistrons Strain(s): 2237, 2238 PubMed: 15956662 DOI: 10.1534/genetics.105.044503 Kimpel, D. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii I. Localization. Plant Cell Physiol., 24, 255-259. Keywords: carbonic anhydrase; cell wall-less mutant; Chyamydomonas reinhardtii; periplasm; wall lysing enzyme Strain(s): 2237, 2238 DOI: 10.1093/pcp/24.2.255 |
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