Strain Data

A big image opens when the thumbnail is clicked.(nies-2235.jpg)

Strain number		NIES-2235
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Chlamydomonas reinhardtii P.A.Dangeard
Synonym		Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941
Former name
Common name		Green alga
Locality (Date of collection)		near Amherst, Massachusetts, USA
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < Tsubo, Yoshihiro (1959) < Sager (1954) < Smith (1953)
Isolator (Date of isolation)
Identified by
State of strain		Subculture / Cryopreservation; Unialgal; Clonal; Axenic[2024 Nov]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 C Light intensity: 21 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 6 M
Gene information		Total genome information ( http://chlamy.pmb.lif.kyoto-u.ac.jp/chlamybase ) , EST information ( http://est.kazusa.or.jp/en/plant/chlamy/EST/index.html ) , Amt protein mRNA(putative) ( AF509497 ) , CAH1 ( D90206 AB026126 ) , CAH2 ( X54488 ) , CrDES ( AB239769 ) , GCS1 ( AB206813 ) , mmp1 ( AB058411 AB058413 ) , mmp2 ( AB058412 AB058414 ) , mmp3 ( AB108850 ) , NSG1 ( AB167472 ) , NSG6 ( AB167474 ) , NSG7 ( AB167475 ) , NSG11 ( AB167476 ) , NSG13 ( AB167477 ) , NSG17 ( AB167478 ) , RECA ( AB048829 ) , SIG1 mRNA ( AB049220 ) , 18S rRNA ( AB701550 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Phototaxis ; Starch production (Kuchitsu et al. 1988) ; Genome decoded strain ; Mating type (−) ; Crosses with NIES-2239 (IAM C-562) ; Live food (Liu et al. 2016)
Other strain no.		Other collection strain no. : IAM C-9 Other strain no. : Tsubo R (−)
Remarks		Cryopreserved; Axenic
Movie

Reference
Nakanishi, A., Yomogita, M., Horimoto, T. 2024 Evaluation of cellular responses by Chlamydomonas reinhardtii in media containing dairy-processing residues derived from cheese as nutrients by analyzing cell growth activity and comprehensive gene transcription levels. Microorganisms, 12, 715 (article ID). Keywords: Chlamydomonas reinhardtii; whey; whey retentate; cellular response; transcriptomics; biomass; mineral; potassium Strain(s): 2235 PubMed: 38674659 DOI: 10.3390/microorganisms12040715 Nakanishi, A., Omino, N., Nakamura, T., Goto, S., Matsumoto, R., Yomogita, M., Narisawa, N., Kimijima, M., Iritani, K. 2024 Evaluation of cellular responses of heterotrophic Escherichia coli cultured with autotrophic Chlamydomonas reinhardtii as a nutrient source by analyses based on microbiology and transcriptome. Microorganisms, 12, 452 (article ID). Keywords: Escherichia coli; Chlamydomonas reinhardtii; cellular response; transcriptomics Strain(s): 2235 PubMed: 38543503 DOI: 10.3390/microorganisms12030452 Mezgebu, A., Liu, X., Mingist, M., Ban, S. 2023 Evaluating food quality of Arthrospira platensis for culturing Daphnia magna. Aquac. Int., 32, 3533-3544. Keywords: Arthrospira platensis; Daphnia magna; Essential biochemicals; Food quality Strain(s): 39, 2235 DOI: 10.1007/s10499-023-01336-9 Nakanishi, A., Nemoto, S., Yamamoto, N., Iritani, K., Watanabe, M. 2023 Identification of cell-attachment factors derived from green algal cells disrupted by sonication in fabrication of cell plastics. Bioengineering-Basel, 10, 893 (article ID). Keywords: cell plastics; unicellular green algae; attachment factors in matrices; proteomics Strain(s): 2235 PubMed: 37627778 DOI: 10.3390/bioengineering10080893 Nakanishi, A., Iritani, K., Tsuruta, A., Yamamoto, N., Watanabe, M., Ozawa, N., Watanabe, M., Zhang, K., Tokudome, A. 2022 Fabrication of cell plastics composed only of unicellular green alga Chlamydomonas reinhardtii as a raw material. Photosynth. Res., 106, 4459–4468. Keywords: Biomass plastics; Cell plastics; Carbon recycling; Green algae; Chlamydomonas reinhardtii; Intracellular contents Strain(s): 2235 PubMed: 35676378 DOI: 10.1007/s00253-022-12000-2 Yamano, T., Toyokawa, C., Shimamura, D., Matsuoka, T., Fukuzawa, H. 2021 CO₂-dependent migration and relocation of LCIB, a pyrenoid-peripheral protein in Chlamydomonas reinhardtii. Plant Physiol., 188, 1081–1094. Strain(s): 2235 PubMed: 34791500 DOI: 10.1093/plphys/kiab528 Nakanishi, A., Iritani, K., Sakihama, Y., Watanabe, M., Mochiduki, A., Tsuruta, A., Sakamoto, S., Ota, A. 2021 Fabrication and biodegradability of starch cell-plastics as recyclable resources. Appl. Sci., 11, 847 (article ID). Keywords: cell-plastics; biodegradability; unicellular green alga; green plastics Strain(s): 2235 DOI: 10.3390/app11020847 Hang, L. T., Mori, K., Toyama, T. 2020 Enhanced biomass and lipid production capacity of Chlamydomonas reinhardtii under mixotrophic cultivation using sewage effluent and waste molasses. Jpn. J. Water Treat. Biol., 56, 57-66. Keywords: Chlamydomonas reinhardtii; lipid production; mixotrophy; sewage effluent; organic waste Strain(s): 2235 DOI: 10.2521/jswtb.56.57 Nakanishi, A., Iritani, K., Sakihama, Y., Ozawa, N., Mochizuki, A., Watanabe, M. 2020 Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer. AMB Express, 10, 112 (article ID). Keywords: Chlamydomonas reinhardtii; Two-dimensional polymer; Stacking structure; Cell-plastics Strain(s): 2235 PubMed: 32524300 DOI: 10.1186/s13568-020-01046-y Hang, L. T., Mori, K., Tanaka, Y., Morikawa, M., Toyama, T. 2020 Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl₂ stresses. Bioprocess. Biosyst. Eng., 43, 971–980. Keywords: Chlamydomonas reinhardtii; NaCl; CaCl₂; Lipid accumulation; Biofuel production Strain(s): 2235 PubMed: 32008095 DOI: 10.1007/s00449-020-02293-w Yamano, T. & Fukuzawa, H. 2020 Transformation of the model microalga Chlamydomonas reinhardtii without cell-wall removal. In Electroporation Protocols. Methods in Molecular Biology, Eds. by Li, S., Chang, L., Teissie, J., Humana, New York, NY, 2050, pp. 155-161. Keywords: Chlamydomonas reinhardtii; Electroporation; Photosynthesis; Protist; Square electric pulse Strain(s): 2235 PubMed: 31468489 DOI: 10.1007/978-1-4939-9740-4_16 Ishinishi, R., Matsuura, H., Tanaka, S., Nozawa, S., Tanada, K., Kawashita, N., Fujiyama, K., Miyasaka, H., Hirata, K. 2018 Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga. Gene, 640, 14-20. Keywords: Stress response; Chlamydomonas reinhardtii; Secretory protein; Recombinant protein; Unknown function Strain(s): 2235 PubMed: 29017964 DOI: 10.1016/j.gene.2017.10.012 Hamada, M., Schröder, K., Bathia, J., Kürn, U., Fraune, S., Khalturina, M., Khalturin, K., Shinzato, C., Satoh, N., Bosch, T. C.G. 2018 Metabolic co-dependence drives the evolutionary ancient Hydra-Chlorella symbiosis. eLife, 7, e35122 (article ID). Keywords: Chlorella; Hydra; evolutionary biology; genome; nitrogen metabolism; symbiosis Strain(s): 2166, 2235, 2541 PubMed: 29848439 DOI: 10.7554/eLife.35122 Toyama, T., Kasuya, M., Hanaoka, T., Kobayashi, N., Tanaka, Y., Inoue, D., Sei, K., Morikawa, M., Mori, K. 2018 Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater efuent. Biotechnol. Biofuels, 11, 176 (article ID). Keywords: Microalgae; Wastewater efuent; Enhanced biomass production; Microalgae growth-promoting bacteria Strain(s): 48, 2172, 2235 PubMed: 29983739 DOI: 10.1186/s13068-018-1174-0 Seto, K. & Degawa, Y. 2018 Collimyces mutans gen. et sp. nov. (Rhizophydiales, Collimycetaceae fam. nov.), a new chytrid parasite of Microglena (Volvocales, clade Monadinia). Protist, 169, 507-520. Keywords: Chytrid; Collimyces mutans; Microglena; parasite; Rhizophydiales; zoospore ultrastructure Strain(s): 755, 858, 2220, 2235, 2743, 2744 PubMed: 29935342 DOI: 10.1016/j.protis.2018.02.006 Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M. 2018 Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N¹-aminopentylspermidine, N⁸-aminopentylspermidine, and penta-amines.— Microb. Resour. Syst., 34, 73-82. Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine Strain(s): 18, 35, 242, 691, 735, 1271, 1278, 2185, 2187, 2235, 2261, 2540, 2572, 3983 Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146. Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase Strain(s): 2202, 2207, 2212, 2235, 2242 PubMed: 2848352 DOI: 10.1016/j.bbrc.2017.05.026 Liu, X., Syuhei, B. 2017 Effects of acclimatization on metabolic plasticity of Eodiaptomus japonicus (Copepoda: Calanoida) determined using an optical oxygen meter. J. Plankton Res., 39, 111-121. Keywords: temperature acclimatization; metabolic rates; oxygen consumption rates; zooplankton; optical oxygen meter Strain(s): 282, 2235 DOI: 10.1093/plankt/fbw084 Kong, F., Yamasaki, T., Kurniasih, S. D., Hou, L., Li, X., Ivanova, N., Okada, S., Ohama, T. 2015 Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains. Biotech. Bioeng., 120, 239-245. Keywords: Transgene silencing; Position effect; Squalene synthase-like gene; Botryococcus braunii; Methyltransferase gene Strain(s): 2235 PubMed: 25660568 DOI: 10.1016/j.jbiosc.2015.01.005 Liu, X., Beyrend-Dur, D., Dur, G., Ban, S. 2015 Combined effects of temperature and food concentration on growth and reproduction of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan). Freshw. Biol., 60, 2003-2018. Keywords: copepod dynamics; egg production; Eodiaptomus japonicus; individual growth rates; temperature–food effects Strain(s): 282, 2235 DOI: 10.1111/fwb.12626 Liu, X., Beyrend-Dur, D., Dur, G., Ban, S. 2014 Effects of temperature on life history traits of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan). Limnology, 15, 85-97. Keywords: Life history traits; Temperature effects; Eodiaptomus japonicus; Copepod dynamics; Individual variability Strain(s): 282, 2235 DOI: 10.1007/s10201-013-0417-9 Wang, L., Yamano, T., Kajikawa, M., Hirono, M., Fukuzawa, H. 2014 Isolation and characterization of novel high-CO₂-requiring mutants of Chlamydomonas reinhardtii. Photosynth. Res., 121, 175-184. Keywords: Chlamydomonas reinhardtii; Carbon-concentrating mechanism; CO₂-signal transduction; High-CO₂-requiring mutants Strain(s): 2235 PubMed: 24549931 DOI: 10.1007/s11120-014-9983-x Yamano, T., Iguchi, H. Fukuzawa, H. 2013 Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal. J. Biosci. Bioeng., 115, 691-694. Keywords: Chlamydomonas reinhardtii; Electroporation; Square electric pulse; Transformation Strain(s): 2235 PubMed: 23333644 DOI: 10.1016/j.jbiosc.2012.12.020 Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12. Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284 Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Bioresour. Technol., 109, 300-303. Keywords: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate Strain(s): 35, 119, 227, 230, 2235 PubMed: 21906932 DOI: 10.1016/j.biortech.2011.07.101 Fujii, K., Nakashima, H., Hashidzume, Y. 2010 Isolation of folate-producing microalgae, from oligotrophic ponds in Yamaguchi, Japan. J. Appl. Microbiol., 108, 1421-1429. Keywords: B-vitamins; Chlamydomonas; Chlorococcum; folate; microalgae; thiamine Strain(s): 2172, 2235, 2848, 2849, 2850, 2851 PubMed: 19796121 DOI: 10.1111/j.1365-2672.2009.04542.x Yamano, T., Miura, K., Fukuzawa, H. 2008 Expression analysis of genes associated with the induction of the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol., 147, 340-354. Strain(s): 2235 PubMed: 18322145 DOI: 10.1104/pp.107.114652 Kajikawa, M., Yamato, K. T., Kohzu, Y., Shoji, S., Sakai, Y., Fukuzawa, H. 2006 A front-end desaturase from the green alga Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by ω13 desaturation in methylotrophic yeast and tobacco. Plant Cell Physiol., 47, 64-73. Keywords: Chlamydomonas reinhardtii; Δ5-unsaturated bis-methylene-interrupted fatty acids; fatty acid desaturase; Pichia pastoris; tobacco plant Strain(s): 2235 PubMed: 16267098 DOI: 10.1093/pcp/pci224 Abe, J., Kubo, T., Takagi, Y., Saito, T., Miura, K., Fukuzawa, H., Matsuda, Y. 2004 The transcriptional program of synchronous gametogenesis in Chlamydomonas reinhardtii. Curr. Genet., 46, 304-315. Strain(s): 2235 PubMed: 15459796 DOI: 10.1007/s00294-004-0526-4 Asamizu, E., Nakamura, Y., Miura, K., Fukuzawa, H., Fujiwara, S., Hirono, M., Iwamoto, K., Matsuda, Y., Minagawa, J., Shimogawara, K., Takahashi, Y., Tabata, S. 2004 Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta), to facilitate gene function analysis. Phycologia, 43, 722-726. Strain(s): 2235 DOI: 10.2216/i0031-8884-43-6-722.1 Miura, K., Yamano, T., Yoshioka, S., Kohinata, T., Inoue, Y., Taniguchi, F., Asamizu, E., Nakamura, Y., Tabata, S., Yamato, K. T., Ohyama, K., Fukuzawa, H. 2004 Expression profiling-based identification of CO₂-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol., 135, 1595-1607. Strain(s): 2235 PubMed: 15235119 DOI: 10.1104/pp.104.041400 Yoshioka, S., Taniguchi, F., Miura, K., Inoue, T., Yamano, T., Fukuzawa, H. 2004 The novel Myb transcription factor LCR1 regulates the CO₂-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii. Plant Cell, 16, 1466-1477. Strain(s): 2235 PubMed: 15155888 DOI: 10.1105/tpc.021162 Kubo, T., Abe, J., Saito, T., Matsuda, Y. 2002 Genealogical relationships among laboratory strains of Chlamydomonas reinhardtii as inferred from matrix metalloprotease genes. Curr. Genet., 41, 115-122. Keywords: Chlamydomonas reinhardtii; Chlamydomonas smithii; matrix metalloprotease genes; molecular phylogeny Strain(s): 2235 PubMed: 12073093 DOI: 10.1007/s00294-002-0284-0 Kubo, T., Saito, T., Fukuzawa, H., Matsuda, Y. 2001 Two tandemly-located matrix metalloprotease genes with different expression patterns in the Chlamydomonas sexual cell cycle. Curr. Genet., 40, 136-143. Keywords: cell wall degradation; Chlamydomonas reinhardtii; gametolysin; zygote-specific gene Strain(s): 2235 PubMed: 11680823 DOI: 10.1007/s002940100239 Asamizu, E., Miura, K., Kucho, K., Inoue, Y., Fukuzawa, H., Ohyama, K., Nakamura, Y., Tabata, S. 2000 Generation of expressed sequence tags from low-CO₂ and high-CO₂ adapted cells of Chlamydomonas reinhardtii. DNA Res., 7, 305-307. Keywords: Chlamydomonas reinhardtii; cDNA; EST; CO2 stress Strain(s): 2235 PubMed: 11089912 DOI: 10.1093/dnares/7.5.305 Asamizu, E., Nakamura, Y., Sato, S., Fukuzawa, H., Tabata, S. 1999 A large scale structure analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res., 6, 369-373. Keywords: green alga; Chlamydomonas reinhardtii; cDNA; EST; normalized library; similarity search Strain(s): 2235 PubMed: 10691129 DOI: 10.1093/dnares/6.6.369 Ban, S. 1992 Effects of photoperiod, temperature, and population density on induction of diapause egg production in Eurytemora affinis (Copepoda: Calanoida) in Lake Ohnuma, Hokkaido, Japan. J. Crustacean Biol., 12, 361-367. Keywords: Eggs; Diapause; Female animals; Photoperiod; Nauplii; Egg production; Lakes; Population density; Autumn; Animals Strain(s): 282, 2235 DOI: 10.2307/1549029 Tachiki, A., Fukuzawa, H., Miyachi, S. 1992 Characterization of carbonic anhydrase isozyme CA2, which is the CAH2 gene product, in Chlamydomonas reinhardtii. Biosci. Biotechnol. Biochem., 56, 794-798. Strain(s): 2235 PubMed: 1368343 DOI: 10.1271/bbb.56.794 Fukuzawa, H., Ishida, S., Miyachi, S. 1991 cDNA cloning and gene expression of carbonic anhydrase in Chlamydomonas reinhardtii. Can. J. Bot., 69, 1088-1096. Keywords: photosynthesis; light regulation; zinc; CO2-concentrating mechanism; intracellular processing Strain(s): 2235 DOI: 10.1139/b91-139 Kamo, T., Shimogawara, K., Fukuzawa, H., Muto, S., Miyachi, S. 1990 Subunit constitution of carbonic anhydrase from Chlamydomonas reinhardtii. Eur. J. Biochem., 192, 557-562. Strain(s): 2235 PubMed: 2120056 DOI: 10.1111/j.1432-1033.1990.tb19261.x Kashino, Y., Enami, I., Satoh, K., Katoh, S. 1990 Immunological cross-reactivity among corresponding proteins of photosystems I and II from widely divergent photosynthetic organisms. Plant Cell Physiol., 31, 479-488. Keywords: antiserum; cross-reactivity; immunoblotting; photosystem; protein Strain(s): 2093, 2138, 2235 DOI: 10.1093/oxfordjournals.pcp.a077935 Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., Kawaguchi, A. 1990 Effects of CO₂ concentration during growth on fatty acid composition in microalgae. Plant Physiol., 93, 851-856. Strain(s): 48, 2093, 2138, 2160, 2235 PubMed: 1666759 DOI: 10.1104/pp.93.3.851 Fukuzawa, H., Fujiwara, S., Yamamoto, Y., Dionisio-Sese, M. L., Miyachi, S. 1990 cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: Regulation by environmental CO₂ concentration. Proc. Nati. Acad. Sci. U. S. A., 87, 4383-4387. Strain(s): 2235 PubMed: 2112252 DOI: 10.1073/pnas.87.11.4383 Ehara, T., Ogasawara, Y., Osafune, T. 1990 Behavior of chloroplast nucleoids during the cell cycle of Chlamydomonas reinhardtii (Chlorophyta) in synchronized culture. J. Phycol., 26, 317-323. Keywords: cell cycle; Chlamydomons; chloroplast division; DAPI; nucleoid Strain(s): 2235 DOI: 10.1111/j.0022-3646.1990.00317.x Dionisio-Sese, M. L., Fukuzawa, H., Miyachi, S. 1990 Light-induced carbonic anhydrase expression in Chlamydomonas reinhardtii. Plant Physiol., 94, 1103-1110. Strain(s): 2235 PubMed: 16667803 DOI: 10.1104/pp.94.3.1103 Fujiwara, S., Fukuzawa, H., Tachiki, A., Miyachi, S. 1990 Structure and differential expression of two genes encoding carbonic anhydrase in Chlamydomonas reinhardtii. Proc. Nati. Acad. Sci. U. S. A., 87, 9779-9783. Strain(s): 2235 PubMed: 2124702 DOI: 10.1073/pnas.87.24.9779 Dionisio, M. L., Tsuzuki, M., Miyachi, S. 1989 Light requirement for carbonic anhydrase induction in Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 207-213. Keywords: blue light; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; light intensity Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a077731 Dionisio, M. L., Tsuzuki, M., Miyachi, S. 1989 Blue light induction of carbonic anhydrase activity in Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 215-219. Keywords: blue light; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; photoreceptor Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a077732 Toguri, T., Muto, S., Mihara, S., Miyachi, S. 1989 Synthesis and degradation of carbonic anhydrase in a synchronized culture of Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 533-539. Keywords: carbonic anhydrase synthesis; Chlamydomonas reinhardtii; synchronous culture Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a077772 Tsuzuki, M. & Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104. Strain(s): 2093, 2138, 2160, 2173, 2235 DOI: 10.1016/0304-3770(89)90051-X Kuchitsu, K., Tsuzuki, M., Miyachi, S. 1988 Changes of starch localization within the chloroplast induced by changes in CO₂ concentration during growth of Chlamydomonas reinhardtii. Independent regulation of pyrenoid starch and stroma starch. Plant Cell Physiol., 29, 1269-1278. Keywords: Chlamydomonas (Chlorophyta); chloroplast; CO2 concentration; pyrenoid; starch; stroma Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a077635 Kuchitsu, K., Tsuzuki, M., Miyachi, S. 1988 Characterization of the pyrenoid isolated from unicellular green alga Chlamydomonas reinhardtii. Particulate form of RuBisCO protein. Protoplasma, 144, 17-24. Keywords: Chlamydomonas; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO); stroma Strain(s): 2235 DOI: 10.1007/BF01320276 Bürger, J., Miyachi, S., Galland, P., Senger, H. 1988 Quantum requirements of photosynthetic oxygen evolution and 77K fluorescence emission spectra in unicellular green algae grown under low- and high-CO₂-conditions. Botanica Acta, 101, 229-232. Strain(s): 2173, 2235 DOI: 10.1111/j.1438-8677.1988.tb00037.x Yagawa, Y., Muto, S., Miyachi, S. 1988 Carbonic anhydrase of Chlamydomonas reinhardtii. Cellular distribution and subunit constitution in two strains. Plant Cell Physiol., 29, 185-188. Keywords: carbonic anhydrase (cellular distribution); Chlamydomonas Strain(s): 2235 Toguri, T., Muto, S., Miyachi, S. 1986 Biosynthesis and intracellular processing of carbonic anhydrase in Chlamydomonas reinhardtii. Eur. J. Biochem., 58, 443-450. Strain(s): 2235 Yagawa, Y., Aizawa, K., Yang, S.-Y., Miyachi, S. 1986 Release of carbonic anhydrase from the cell surface of Chlamydomonas reinhardtii by trypsin. Plant Cell Physiol., 27, 215-221. Keywords: carbonic anhydrase; cell surface; Chlamydomonas reinhardtii; trypsin treatment Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a077093 Aizawa, K. & Miyachi, S. 1986 Carbonic anhydrase and CO₂ concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Lett., 39, 215-233. Keywords: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport Strain(s): 2093, 2160, 2173, 2235 DOI: 10.1111/j.1574-6968.1986.tb01860.x Yang, S.-Y., Tsuzuki, M., Miyachi, S. 1985 Carbonic anhydrase of Chlamydomonas: Pulification and studies on its induction using antiserum against Chlamydomonas carbonic anhydrase. Plant Cell Physiol., 26, 25-34. Keywords: affinity chromatography; carbon dioxide concentration; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a076892 Miyachi, S., Tsuzuki, M., Aizawa, K. 1985 Factors which enhance photosynthesis in microalgae under limiting CO₂ concentrations. In Kinetics of Photosynthetic Carbon Metabolism in C₃-plants. II. Electron Transport, Transport of CO₂, Mathematical Models, Eds. by Viil, J. et al., Akademiya Nauka, Estonia SSR, pp. 88-92. Strain(s): 2160, 2235 Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title). Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579 Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240. Strain(s): 687, 2160, 2161, 2162, 2170, 2173, 2235, 2280 DOI: 10.1111/j.1749-6632.1984.tb12341.x Tsubo, Y. & Matsuda, Y. 1984 Transmission of chloroplast genes in crosses between Chlamydomonas reinhardtii diploids: Correlation with chloroplast nucleoid behavior in young zygotes. Curr. Genet., 8, 223-229. Keywords: Chlamydomonas; tetraploid; chloroplast gene transmission; DAPI staining Strain(s): 2235, 2239 PubMed: 24177743 DOI: 10.1007/BF00417820 Tsuzuki, M. 1983 Mode of HCO₃-utilization by the cells of Chlamydomonas reinhardtii grown under ordinary air. Z. Pflanzenphysiol., 110, 29-37. Strain(s): 2160, 2170, 2235 Mihara, S. & Hase, E. 1978 Studies on the vegetative life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. V. The transition point for the effect of 6-methyl purine in the cell cycle. Plant Cell Physiol., 19, 83-89. Keywords: cell cycle; Chlamydomonas; 6-methyl purine; transition point Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a075582 Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1976 Electron microscope studies of the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. IV. Mitochondria in dividing cells. J. Electr. Microsc., 25, 261-269. Strain(s): 2235 Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1975 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. III. Three-dimensional structures of mitochondria in the cells at intermediate stages of the growth phase of the cell cycle. J. Electr. Microsc., 24, 247-252. Strain(s): 2235 Mihara, S. & Hase, E. 1975 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. III. Some notes on the process of zoospore liberation. Plant Cell Physiol., 16, 371-375. Strain(s): 2235 Mihara, S. & Hase, E. 1975 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. IV. Effects of 6-methyl purine on the revolution of the cell cycle. Plant Cell Physiol., 16, 1121-1130. Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a075231 Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1972 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. II. Association of mitochondria and the chloroplast at an early developmental stage. Plant Cell Physiol., 13, 981-989. Strain(s): 2235 Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1972 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. I. Some characteristics of changes in subcellular structures during the cell cycle, especially in formation of giant mitochondria. Plant Cell Physiol., 13, 211-227. Strain(s): 2235 DOI: 10.1093/oxfordjournals.pcp.a074732 Mihara, S. & Hase, E. 1971 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. I. Some characteristics of the cell cycle. Plant Cell Physiol., 12, 225-236. Strain(s): 2235 Mihara, S. & Hase, E. 1971 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. II. Effects of chloramphenicol and cycloheximide on the length of cell cycle. Plant Cell Physiol., 12, 237-241. Strain(s): 2235

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Mezgebu, A., Liu, X., Mingist, M., Ban, S. 2023 Evaluating food quality of Arthrospira platensis for culturing Daphnia magna. Aquac. Int., 32, 3533-3544.
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Nakanishi, A., Iritani, K., Sakihama, Y., Ozawa, N., Mochizuki, A., Watanabe, M. 2020 Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer. AMB Express, 10, 112 (article ID).
Keywords: Chlamydomonas reinhardtii; Two-dimensional polymer; Stacking structure; Cell-plastics
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Hang, L. T., Mori, K., Tanaka, Y., Morikawa, M., Toyama, T. 2020 Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl₂ stresses. Bioprocess. Biosyst. Eng., 43, 971–980.
Keywords: Chlamydomonas reinhardtii; NaCl; CaCl₂; Lipid accumulation; Biofuel production
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Yamano, T. & Fukuzawa, H. 2020 Transformation of the model microalga Chlamydomonas reinhardtii without cell-wall removal. In Electroporation Protocols. Methods in Molecular Biology, Eds. by Li, S., Chang, L., Teissie, J., Humana, New York, NY, 2050, pp. 155-161.
Keywords: Chlamydomonas reinhardtii; Electroporation; Photosynthesis; Protist; Square electric pulse
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Ishinishi, R., Matsuura, H., Tanaka, S., Nozawa, S., Tanada, K., Kawashita, N., Fujiyama, K., Miyasaka, H., Hirata, K. 2018 Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga. Gene, 640, 14-20.
Keywords: Stress response; Chlamydomonas reinhardtii; Secretory protein; Recombinant protein; Unknown function
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Hamada, M., Schröder, K., Bathia, J., Kürn, U., Fraune, S., Khalturina, M., Khalturin, K., Shinzato, C., Satoh, N., Bosch, T. C.G. 2018 Metabolic co-dependence drives the evolutionary ancient Hydra-Chlorella symbiosis. eLife, 7, e35122 (article ID).
Keywords: Chlorella; Hydra; evolutionary biology; genome; nitrogen metabolism; symbiosis
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Toyama, T., Kasuya, M., Hanaoka, T., Kobayashi, N., Tanaka, Y., Inoue, D., Sei, K., Morikawa, M., Mori, K. 2018 Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater efuent. Biotechnol. Biofuels, 11, 176 (article ID).
Keywords: Microalgae; Wastewater efuent; Enhanced biomass production; Microalgae growth-promoting bacteria
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Seto, K. & Degawa, Y. 2018 Collimyces mutans gen. et sp. nov. (Rhizophydiales, Collimycetaceae fam. nov.), a new chytrid parasite of Microglena (Volvocales, clade Monadinia). Protist, 169, 507-520.
Keywords: Chytrid; Collimyces mutans; Microglena; parasite; Rhizophydiales; zoospore ultrastructure
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Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M. 2018 Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N¹-aminopentylspermidine, N⁸-aminopentylspermidine, and penta-amines.— Microb. Resour. Syst., 34, 73-82.
Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine
Strain(s): 18, 35, 242, 691, 735, 1271, 1278, 2185, 2187, 2235, 2261, 2540, 2572, 3983

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
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Liu, X., Syuhei, B. 2017 Effects of acclimatization on metabolic plasticity of Eodiaptomus japonicus (Copepoda: Calanoida) determined using an optical oxygen meter. J. Plankton Res., 39, 111-121.
Keywords: temperature acclimatization; metabolic rates; oxygen consumption rates; zooplankton; optical oxygen meter
Strain(s): 282, 2235
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Kong, F., Yamasaki, T., Kurniasih, S. D., Hou, L., Li, X., Ivanova, N., Okada, S., Ohama, T. 2015 Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains. Biotech. Bioeng., 120, 239-245.
Keywords: Transgene silencing; Position effect; Squalene synthase-like gene; Botryococcus braunii; Methyltransferase gene
Strain(s): 2235
PubMed: 25660568
DOI: 10.1016/j.jbiosc.2015.01.005

Liu, X., Beyrend-Dur, D., Dur, G., Ban, S. 2015 Combined effects of temperature and food concentration on growth and reproduction of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan). Freshw. Biol., 60, 2003-2018.
Keywords: copepod dynamics; egg production; Eodiaptomus japonicus; individual growth rates; temperature–food effects
Strain(s): 282, 2235
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Liu, X., Beyrend-Dur, D., Dur, G., Ban, S. 2014 Effects of temperature on life history traits of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan). Limnology, 15, 85-97.
Keywords: Life history traits; Temperature effects; Eodiaptomus japonicus; Copepod dynamics; Individual variability
Strain(s): 282, 2235
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Wang, L., Yamano, T., Kajikawa, M., Hirono, M., Fukuzawa, H. 2014 Isolation and characterization of novel high-CO₂-requiring mutants of Chlamydomonas reinhardtii. Photosynth. Res., 121, 175-184.
Keywords: Chlamydomonas reinhardtii; Carbon-concentrating mechanism; CO₂-signal transduction; High-CO₂-requiring mutants
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Yamano, T., Iguchi, H. Fukuzawa, H. 2013 Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal. J. Biosci. Bioeng., 115, 691-694.
Keywords: Chlamydomonas reinhardtii; Electroporation; Square electric pulse; Transformation
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Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284

Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Bioresour. Technol., 109, 300-303.
Keywords: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate
Strain(s): 35, 119, 227, 230, 2235
PubMed: 21906932
DOI: 10.1016/j.biortech.2011.07.101

Fujii, K., Nakashima, H., Hashidzume, Y. 2010 Isolation of folate-producing microalgae, from oligotrophic ponds in Yamaguchi, Japan. J. Appl. Microbiol., 108, 1421-1429.
Keywords: B-vitamins; Chlamydomonas; Chlorococcum; folate; microalgae; thiamine
Strain(s): 2172, 2235, 2848, 2849, 2850, 2851
PubMed: 19796121
DOI: 10.1111/j.1365-2672.2009.04542.x

Yamano, T., Miura, K., Fukuzawa, H. 2008 Expression analysis of genes associated with the induction of the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol., 147, 340-354.
Strain(s): 2235
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Kajikawa, M., Yamato, K. T., Kohzu, Y., Shoji, S., Sakai, Y., Fukuzawa, H. 2006 A front-end desaturase from the green alga Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by ω13 desaturation in methylotrophic yeast and tobacco. Plant Cell Physiol., 47, 64-73.
Keywords: Chlamydomonas reinhardtii; Δ5-unsaturated bis-methylene-interrupted fatty acids; fatty acid desaturase; Pichia pastoris; tobacco plant
Strain(s): 2235
PubMed: 16267098
DOI: 10.1093/pcp/pci224

Abe, J., Kubo, T., Takagi, Y., Saito, T., Miura, K., Fukuzawa, H., Matsuda, Y. 2004 The transcriptional program of synchronous gametogenesis in Chlamydomonas reinhardtii. Curr. Genet., 46, 304-315.
Strain(s): 2235
PubMed: 15459796
DOI: 10.1007/s00294-004-0526-4

Asamizu, E., Nakamura, Y., Miura, K., Fukuzawa, H., Fujiwara, S., Hirono, M., Iwamoto, K., Matsuda, Y., Minagawa, J., Shimogawara, K., Takahashi, Y., Tabata, S. 2004 Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta), to facilitate gene function analysis. Phycologia, 43, 722-726.
Strain(s): 2235
DOI: 10.2216/i0031-8884-43-6-722.1

Miura, K., Yamano, T., Yoshioka, S., Kohinata, T., Inoue, Y., Taniguchi, F., Asamizu, E., Nakamura, Y., Tabata, S., Yamato, K. T., Ohyama, K., Fukuzawa, H. 2004 Expression profiling-based identification of CO₂-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol., 135, 1595-1607.
Strain(s): 2235
PubMed: 15235119
DOI: 10.1104/pp.104.041400

Yoshioka, S., Taniguchi, F., Miura, K., Inoue, T., Yamano, T., Fukuzawa, H. 2004 The novel Myb transcription factor LCR1 regulates the CO₂-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii. Plant Cell, 16, 1466-1477.
Strain(s): 2235
PubMed: 15155888
DOI: 10.1105/tpc.021162

Kubo, T., Abe, J., Saito, T., Matsuda, Y. 2002 Genealogical relationships among laboratory strains of Chlamydomonas reinhardtii as inferred from matrix metalloprotease genes. Curr. Genet., 41, 115-122.
Keywords: Chlamydomonas reinhardtii; Chlamydomonas smithii; matrix metalloprotease genes; molecular phylogeny
Strain(s): 2235
PubMed: 12073093
DOI: 10.1007/s00294-002-0284-0

Kubo, T., Saito, T., Fukuzawa, H., Matsuda, Y. 2001 Two tandemly-located matrix metalloprotease genes with different expression patterns in the Chlamydomonas sexual cell cycle. Curr. Genet., 40, 136-143.
Keywords: cell wall degradation; Chlamydomonas reinhardtii; gametolysin; zygote-specific gene
Strain(s): 2235
PubMed: 11680823
DOI: 10.1007/s002940100239

Asamizu, E., Miura, K., Kucho, K., Inoue, Y., Fukuzawa, H., Ohyama, K., Nakamura, Y., Tabata, S. 2000 Generation of expressed sequence tags from low-CO₂ and high-CO₂ adapted cells of Chlamydomonas reinhardtii. DNA Res., 7, 305-307.
Keywords: Chlamydomonas reinhardtii; cDNA; EST; CO2 stress
Strain(s): 2235
PubMed: 11089912
DOI: 10.1093/dnares/7.5.305

Asamizu, E., Nakamura, Y., Sato, S., Fukuzawa, H., Tabata, S. 1999 A large scale structure analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res., 6, 369-373.
Keywords: green alga; Chlamydomonas reinhardtii; cDNA; EST; normalized library; similarity search
Strain(s): 2235
PubMed: 10691129
DOI: 10.1093/dnares/6.6.369

Ban, S. 1992 Effects of photoperiod, temperature, and population density on induction of diapause egg production in Eurytemora affinis (Copepoda: Calanoida) in Lake Ohnuma, Hokkaido, Japan. J. Crustacean Biol., 12, 361-367.
Keywords: Eggs; Diapause; Female animals; Photoperiod; Nauplii; Egg production; Lakes; Population density; Autumn; Animals
Strain(s): 282, 2235
DOI: 10.2307/1549029

Tachiki, A., Fukuzawa, H., Miyachi, S. 1992 Characterization of carbonic anhydrase isozyme CA2, which is the CAH2 gene product, in Chlamydomonas reinhardtii. Biosci. Biotechnol. Biochem., 56, 794-798.
Strain(s): 2235
PubMed: 1368343
DOI: 10.1271/bbb.56.794

Fukuzawa, H., Ishida, S., Miyachi, S. 1991 cDNA cloning and gene expression of carbonic anhydrase in Chlamydomonas reinhardtii. Can. J. Bot., 69, 1088-1096.
Keywords: photosynthesis; light regulation; zinc; CO2-concentrating mechanism; intracellular processing
Strain(s): 2235
DOI: 10.1139/b91-139

Kamo, T., Shimogawara, K., Fukuzawa, H., Muto, S., Miyachi, S. 1990 Subunit constitution of carbonic anhydrase from Chlamydomonas reinhardtii. Eur. J. Biochem., 192, 557-562.
Strain(s): 2235
PubMed: 2120056
DOI: 10.1111/j.1432-1033.1990.tb19261.x

Kashino, Y., Enami, I., Satoh, K., Katoh, S. 1990 Immunological cross-reactivity among corresponding proteins of photosystems I and II from widely divergent photosynthetic organisms. Plant Cell Physiol., 31, 479-488.
Keywords: antiserum; cross-reactivity; immunoblotting; photosystem; protein
Strain(s): 2093, 2138, 2235
DOI: 10.1093/oxfordjournals.pcp.a077935

Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., Kawaguchi, A. 1990 Effects of CO₂ concentration during growth on fatty acid composition in microalgae. Plant Physiol., 93, 851-856.
Strain(s): 48, 2093, 2138, 2160, 2235
PubMed: 1666759
DOI: 10.1104/pp.93.3.851

Fukuzawa, H., Fujiwara, S., Yamamoto, Y., Dionisio-Sese, M. L., Miyachi, S. 1990 cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: Regulation by environmental CO₂ concentration. Proc. Nati. Acad. Sci. U. S. A., 87, 4383-4387.
Strain(s): 2235
PubMed: 2112252
DOI: 10.1073/pnas.87.11.4383

Ehara, T., Ogasawara, Y., Osafune, T. 1990 Behavior of chloroplast nucleoids during the cell cycle of Chlamydomonas reinhardtii (Chlorophyta) in synchronized culture. J. Phycol., 26, 317-323.
Keywords: cell cycle; Chlamydomons; chloroplast division; DAPI; nucleoid
Strain(s): 2235
DOI: 10.1111/j.0022-3646.1990.00317.x

Dionisio-Sese, M. L., Fukuzawa, H., Miyachi, S. 1990 Light-induced carbonic anhydrase expression in Chlamydomonas reinhardtii. Plant Physiol., 94, 1103-1110.
Strain(s): 2235
PubMed: 16667803
DOI: 10.1104/pp.94.3.1103

Fujiwara, S., Fukuzawa, H., Tachiki, A., Miyachi, S. 1990 Structure and differential expression of two genes encoding carbonic anhydrase in Chlamydomonas reinhardtii. Proc. Nati. Acad. Sci. U. S. A., 87, 9779-9783.
Strain(s): 2235
PubMed: 2124702
DOI: 10.1073/pnas.87.24.9779

Dionisio, M. L., Tsuzuki, M., Miyachi, S. 1989 Light requirement for carbonic anhydrase induction in Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 207-213.
Keywords: blue light; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; light intensity
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a077731

Dionisio, M. L., Tsuzuki, M., Miyachi, S. 1989 Blue light induction of carbonic anhydrase activity in Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 215-219.
Keywords: blue light; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; photoreceptor
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a077732

Toguri, T., Muto, S., Mihara, S., Miyachi, S. 1989 Synthesis and degradation of carbonic anhydrase in a synchronized culture of Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 533-539.
Keywords: carbonic anhydrase synthesis; Chlamydomonas reinhardtii; synchronous culture
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a077772

Tsuzuki, M. & Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104.
Strain(s): 2093, 2138, 2160, 2173, 2235
DOI: 10.1016/0304-3770(89)90051-X

Kuchitsu, K., Tsuzuki, M., Miyachi, S. 1988 Changes of starch localization within the chloroplast induced by changes in CO₂ concentration during growth of Chlamydomonas reinhardtii. Independent regulation of pyrenoid starch and stroma starch. Plant Cell Physiol., 29, 1269-1278.
Keywords: Chlamydomonas (Chlorophyta); chloroplast; CO2 concentration; pyrenoid; starch; stroma
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a077635

Kuchitsu, K., Tsuzuki, M., Miyachi, S. 1988 Characterization of the pyrenoid isolated from unicellular green alga Chlamydomonas reinhardtii. Particulate form of RuBisCO protein. Protoplasma, 144, 17-24.
Keywords: Chlamydomonas; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO); stroma
Strain(s): 2235
DOI: 10.1007/BF01320276

Bürger, J., Miyachi, S., Galland, P., Senger, H. 1988 Quantum requirements of photosynthetic oxygen evolution and 77K fluorescence emission spectra in unicellular green algae grown under low- and high-CO₂-conditions. Botanica Acta, 101, 229-232.
Strain(s): 2173, 2235
DOI: 10.1111/j.1438-8677.1988.tb00037.x

Yagawa, Y., Muto, S., Miyachi, S. 1988 Carbonic anhydrase of Chlamydomonas reinhardtii. Cellular distribution and subunit constitution in two strains. Plant Cell Physiol., 29, 185-188.
Keywords: carbonic anhydrase (cellular distribution); Chlamydomonas
Strain(s): 2235

Toguri, T., Muto, S., Miyachi, S. 1986 Biosynthesis and intracellular processing of carbonic anhydrase in Chlamydomonas reinhardtii. Eur. J. Biochem., 58, 443-450.
Strain(s): 2235

Yagawa, Y., Aizawa, K., Yang, S.-Y., Miyachi, S. 1986 Release of carbonic anhydrase from the cell surface of Chlamydomonas reinhardtii by trypsin. Plant Cell Physiol., 27, 215-221.
Keywords: carbonic anhydrase; cell surface; Chlamydomonas reinhardtii; trypsin treatment
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a077093

Aizawa, K. & Miyachi, S. 1986 Carbonic anhydrase and CO₂ concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Lett., 39, 215-233.
Keywords: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport
Strain(s): 2093, 2160, 2173, 2235
DOI: 10.1111/j.1574-6968.1986.tb01860.x

Yang, S.-Y., Tsuzuki, M., Miyachi, S. 1985 Carbonic anhydrase of Chlamydomonas: Pulification and studies on its induction using antiserum against Chlamydomonas carbonic anhydrase. Plant Cell Physiol., 26, 25-34.
Keywords: affinity chromatography; carbon dioxide concentration; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a076892

Miyachi, S., Tsuzuki, M., Aizawa, K. 1985 Factors which enhance photosynthesis in microalgae under limiting CO₂ concentrations. In Kinetics of Photosynthetic Carbon Metabolism in C₃-plants. II. Electron Transport, Transport of CO₂, Mathematical Models, Eds. by Viil, J. et al., Akademiya Nauka, Estonia SSR, pp. 88-92.
Strain(s): 2160, 2235

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 18, 19, 21, 24, 26, 29, 31, 39, 40, 41, 44, 45, 46, 50, 73, 74, 75, 76, 77, 80, 81, 87, 88, 89, 90, 91, 93, 95, 98, 99, 100, 101, 120, 122, 131, 135, 137, 144, 151, 204, 207, 213, 257, 263, 379, 415, 2219, 2235, 2239, 2579

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 687, 2160, 2161, 2162, 2170, 2173, 2235, 2280
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Tsubo, Y. & Matsuda, Y. 1984 Transmission of chloroplast genes in crosses between Chlamydomonas reinhardtii diploids: Correlation with chloroplast nucleoid behavior in young zygotes. Curr. Genet., 8, 223-229.
Keywords: Chlamydomonas; tetraploid; chloroplast gene transmission; DAPI staining
Strain(s): 2235, 2239
PubMed: 24177743
DOI: 10.1007/BF00417820

Tsuzuki, M. 1983 Mode of HCO₃-utilization by the cells of Chlamydomonas reinhardtii grown under ordinary air. Z. Pflanzenphysiol., 110, 29-37.
Strain(s): 2160, 2170, 2235

Mihara, S. & Hase, E. 1978 Studies on the vegetative life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. V. The transition point for the effect of 6-methyl purine in the cell cycle. Plant Cell Physiol., 19, 83-89.
Keywords: cell cycle; Chlamydomonas; 6-methyl purine; transition point
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a075582

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1976 Electron microscope studies of the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. IV. Mitochondria in dividing cells. J. Electr. Microsc., 25, 261-269.
Strain(s): 2235

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1975 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. III. Three-dimensional structures of mitochondria in the cells at intermediate stages of the growth phase of the cell cycle. J. Electr. Microsc., 24, 247-252.
Strain(s): 2235

Mihara, S. & Hase, E. 1975 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. III. Some notes on the process of zoospore liberation. Plant Cell Physiol., 16, 371-375.
Strain(s): 2235

Mihara, S. & Hase, E. 1975 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. IV. Effects of 6-methyl purine on the revolution of the cell cycle. Plant Cell Physiol., 16, 1121-1130.
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a075231

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1972 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. II. Association of mitochondria and the chloroplast at an early developmental stage. Plant Cell Physiol., 13, 981-989.
Strain(s): 2235

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1972 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. I. Some characteristics of changes in subcellular structures during the cell cycle, especially in formation of giant mitochondria. Plant Cell Physiol., 13, 211-227.
Strain(s): 2235
DOI: 10.1093/oxfordjournals.pcp.a074732

Mihara, S. & Hase, E. 1971 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. I. Some characteristics of the cell cycle. Plant Cell Physiol., 12, 225-236.
Strain(s): 2235

Mihara, S. & Hase, E. 1971 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. II. Effects of chloramphenicol and cycloheximide on the length of cell cycle. Plant Cell Physiol., 12, 237-241.
Strain(s): 2235

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