Strain Data

Strain number		NIES-3744
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Dunaliella sp.
Synonym
Former name
Common name		Green alga
Locality (Date of collection)		Lake Funazoko-ike Skarvsnes Antarctica (1975-01-25)
Latitude / Longitude		/ 39.55
Habitat (Isolation source)		Brackish water (Lake water)
History		< Kikuchi, Mikio < Watanuki, Tomohiko
Isolator (Date of isolation)		Watanuki, Tomohiko (1987-07-14)
Identified by		Watanuki, Tomohiko
State of strain		Subculture; Unialgal; Non-clonal; Non-axenic
Culture condition (Preculture condition)		Medium: Modified Johnson's medium Temperature: 20 C Light intensity: 25 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M
Gene information
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Water bloom ; Halophilic
Other strain no.		Other strain no. : hunazoko
Remarks
Movie

Reference
Tsutsumi, N., Kikuchi, M., Sawai, J. 2015 Development of algal growth inhibition test with saltwater alga Dunaliella sp. : Toxicity of 39 chemicals. Jpn. J. Environ. Toxicol., 18, 73-83 (in Japanese with English summary). Keywords: saltwater alga; Dunaliella; herbicide; toxicity; log P_ow, susceptibility Strain(s): 3744 Mihirogi, M., Kikuchi, M., Sawai, J. 2012 Development of screening algal growth inhibition test with Dunaliella sp. Jpn. J. Environ. Toxicol., 15, 11-16 (in Japanese with English summary). Strain(s): 3744 DOI: 10.11403/jset.15.11 Watanuki, T. 1998 Organisms as measured by nuclear magnetic resonance spectrometer. Bull. Kanagawa P. H. Lab., 28, 1-15 (in Japanese). Keywords: ¹H-NMR; ¹³C-NMR; ³¹P-NMR; ¹³⁷Cs-NMR Strain(s): 3744 Utsunomiya, A., Watanuki, T., Matsushita, K., Tomita, I 1997 Toxic effects of linear alkylbenzene sulfonate, quaternaryalkylammonium chloride and their complexes on Dunaliella sp. and Chlorella pyrenoidosa. Environ. Toxicol. Chem., 16, 1247-1254. Keywords: Linear alkylbenzene sulfonate; Cationic surfactant; Complex Dunaliella Chlorella Strain(s): 3744 DOI: 10.1002/etc.5620160621 Utsunomiya, A., Watanuki, T., Matsushita, K., Nishina, M., Tomita, I 1997 Assessment of the toxicity of linear alkylbenzene sulfonate and quaternary alkylammonium chloride by measuring ¹³C-glycerol in Dunaliella sp. Chemosphere, 35, 2479-2490. Keywords: Surfactant; Toxicity; LAS; Quaternary ammonium chloride; Glycerol; ¹³C-glycerol; Dunaliella sp; ¹³C-NMR; EC50-24h Strain(s): 3744 DOI: 10.1016/S0045-6535(97)00316-0 Watanuki, T., Iijima, I., Matsushita, K., Nishina, M., Sumiya, K., Kato, K. 1996 Incorporation of ¹³C labelled carbon into glycerol in the halotolerant alga Dunaliella. JSMRM, 7, 109-112 (in Japanese). Keywords: halotolerant species; ¹³C-enriched sodium bicarbonate; glycerol Strain(s): 3744 Watanuki, T., Matsushita, K., Nishina, M., Hori, E., Kato, K. 1994 J. Phys. Fit. Nutr. Immunol., 40-44 (in Japanese). Strain(s): 3744 Watanuki, T. 1994 Response to salt stress of Antarctic Dunaliella, as measured by nuclear magnetic resonance spectroscopy. JSMRM, 5, 118-120 (in Japanese). Keywords: antarctic; Dunaliella; high salt lake; salt stress; NMR; glycerol Strain(s): 3744 Watanuki, T., Matsushita, K., Kato, K. 1993 Response of Antarctic alga to salt stress, as measured by nuclear magnetic resonance spectroscopy. Seibutsu-Butsuri, 33, 38-41 (in Japanese). Strain(s): 3744 DOI: 10.2142/biophys.33.228 Watanuki, T., Ohno, M., Nakamura, S. 1987 Growth characteristics of Dunaliella sp., unicellular green alga isolated from a salt lake along the coastal region of Lüetzow-Holm bay, Antarctica. Rep. Usa mar. biol. Inst., Kouchi Univ., 9, 139-147. Keywords: Dunaliella sp.; Green algae; Saline lake; Antarctica Strain(s): 3744 Watanuki, T. 1979 Isolation and culture of Antarctic diatoms from the saline lakes in the Soya coast, east Antarctica. Memoirs of National Institute of Polar Research, Special issue 11, 35-41. Strain(s): 3744

Reference

Tsutsumi, N., Kikuchi, M., Sawai, J. 2015 Development of algal growth inhibition test with saltwater alga Dunaliella sp. : Toxicity of 39 chemicals. Jpn. J. Environ. Toxicol., 18, 73-83 (in Japanese with English summary).
Keywords: saltwater alga; Dunaliella; herbicide; toxicity; log P_ow, susceptibility
Strain(s): 3744

Mihirogi, M., Kikuchi, M., Sawai, J. 2012 Development of screening algal growth inhibition test with Dunaliella sp. Jpn. J. Environ. Toxicol., 15, 11-16 (in Japanese with English summary).
Strain(s): 3744
DOI: 10.11403/jset.15.11

Watanuki, T. 1998 Organisms as measured by nuclear magnetic resonance spectrometer. Bull. Kanagawa P. H. Lab., 28, 1-15 (in Japanese).
Keywords: ¹H-NMR; ¹³C-NMR; ³¹P-NMR; ¹³⁷Cs-NMR
Strain(s): 3744

Utsunomiya, A., Watanuki, T., Matsushita, K., Tomita, I 1997 Toxic effects of linear alkylbenzene sulfonate, quaternaryalkylammonium chloride and their complexes on Dunaliella sp. and Chlorella pyrenoidosa. Environ. Toxicol. Chem., 16, 1247-1254.
Keywords: Linear alkylbenzene sulfonate; Cationic surfactant; Complex Dunaliella Chlorella
Strain(s): 3744
DOI: 10.1002/etc.5620160621

Utsunomiya, A., Watanuki, T., Matsushita, K., Nishina, M., Tomita, I 1997 Assessment of the toxicity of linear alkylbenzene sulfonate and quaternary alkylammonium chloride by measuring ¹³C-glycerol in Dunaliella sp. Chemosphere, 35, 2479-2490.
Keywords: Surfactant; Toxicity; LAS; Quaternary ammonium chloride; Glycerol; ¹³C-glycerol; Dunaliella sp; ¹³C-NMR; EC50-24h
Strain(s): 3744
DOI: 10.1016/S0045-6535(97)00316-0

Watanuki, T., Iijima, I., Matsushita, K., Nishina, M., Sumiya, K., Kato, K. 1996 Incorporation of ¹³C labelled carbon into glycerol in the halotolerant alga Dunaliella. JSMRM, 7, 109-112 (in Japanese).
Keywords: halotolerant species; ¹³C-enriched sodium bicarbonate; glycerol
Strain(s): 3744

Watanuki, T., Matsushita, K., Nishina, M., Hori, E., Kato, K. 1994 J. Phys. Fit. Nutr. Immunol., 40-44 (in Japanese).
Strain(s): 3744

Watanuki, T. 1994 Response to salt stress of Antarctic Dunaliella, as measured by nuclear magnetic resonance spectroscopy. JSMRM, 5, 118-120 (in Japanese).
Keywords: antarctic; Dunaliella; high salt lake; salt stress; NMR; glycerol
Strain(s): 3744

Watanuki, T., Matsushita, K., Kato, K. 1993 Response of Antarctic alga to salt stress, as measured by nuclear magnetic resonance spectroscopy. Seibutsu-Butsuri, 33, 38-41 (in Japanese).
Strain(s): 3744
DOI: 10.2142/biophys.33.228

Watanuki, T., Ohno, M., Nakamura, S. 1987 Growth characteristics of Dunaliella sp., unicellular green alga isolated from a salt lake along the coastal region of Lüetzow-Holm bay, Antarctica. Rep. Usa mar. biol. Inst., Kouchi Univ., 9, 139-147.
Keywords: Dunaliella sp.; Green algae; Saline lake; Antarctica
Strain(s): 3744

Watanuki, T. 1979 Isolation and culture of Antarctic diatoms from the saline lakes in the Soya coast, east Antarctica. Memoirs of National Institute of Polar Research, Special issue 11, 35-41.
Strain(s): 3744

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