Strain number NIES-2267  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Monoraphidium sp.  
Former name  
Common name Green alga  
Locality (Date of collection) Yamaguchi, Japan  
Latitude / Longitude  
Habitat (Isolation source) (Activated sludge)  
History < IAM (2007) < Fujii, Katsuhiko  
Isolator (Date of isolation) Fujii, Katsuhiko  
Identified by Fujii, Katsuhiko  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information  
Cell size (min - max) - 200 μm  
Organization Unicellular 
Characteristics Astaxanthin production  
Other strain no. Other collection strain no. : IAM C-632
Other strain no. : GK 12  
Remarks Cryopreserved; Axenic 
Fujii, K. 2012 Process integration of supercritical carbon dioxide extraction and acid treatment for astaxanthin extraction from a vegetative microalga. Food Bioprod. Process., 90, 762-766.
Keywords: Supercritical carbon dioxide; Microalgae; Monoraphidium sp.; Astaxanthin; Chlorophyll
Strain(s): 2267 
DOI: 10.1016/j.fbp.2012.01.006

Fujii, K., Nakashima, H., Hashidzume, Y., Uchiyama, T., Mishiro, K., Kadota, Y. 2010 Potential use of the astaxanthin-producing microalga, Monoraphidium sp. GK12, as a functional aquafeed for prawns. J. Appl. Phycol., 22, 363-369.
Keywords: Astaxanthin; Vitamin; Unsaturated fatty acid; Microalgae; Monoraphidium; Functional aquafeed
Strain(s): 2267 
DOI: 10.1007/s10811-009-9468-z

Fujii, K., Nakajima, H, Anno, Y. 2008 Potential of Monoraphidium sp. GK12 for energy-saving astazanthin production. J. Chem. Tech. Biotechnol., 83, 1578-1584.
Keywords: astaxanthin; microalgae; Monoraphidium sp.; outdoor cultivation; energy-saving technology
Strain(s): 2267 
DOI: 10.1002/jctb.1978

Fujii, K., Imazato, E., Nakashima, H., Ooi, O., Saeki, A. 2006 Isolation of the non-fastidious microalga with astaxanthin-accumulating property and its potential for application to aquaculture. Aquaculture, 261, 285-293.
Keywords: Astaxanthin; Aquaculture; Microalgae; Monoraphidium
Strain(s): 2267 
DOI: 10.1016/j.aquaculture.2006.07.014

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