Strain number NIES-2258  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Dunaliella tertiolecta Butcher  
Former name  
Common name Green alga  
Locality (Date of collection) Oslofjord, Norway  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Hara, Yoshiaki (1989) < UTEX  
Isolator (Date of isolation) Foyn, B. (1938-**-**)  
Identified by  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  20 C
Light intensity:  6 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information  
Cell size (min - max) 10 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics Starch production (Shimonaga, T. 2008)  
Other strain no. Other collection strain no. : IAM C-524; UTEX LB 999; CCAP 19/6B
Remarks Axenic 
Komatsu, K., Onouchi, H., Imai, A., Kawasaki, N., Hashim, E. F., Mohd Rajuddin, M. K. 2019 Effects of dissolved organic matter in soil extracts on the growth of microalgae. J. Jpn. Soc. Wat. Environ., 42, 239-246 (in Japanese with English summary).
Keywords: Soil extracts; Microalgae; Microplate technique; Excitation emission matrix; Molecular size distribution
Strain(s): 39214521682170225622572258 
DOI: 10.2965/jswe.42.239

Yamane, K., Matsuyama, S., Igarashi, K., Utsumi, M., Shiraiwa, Y., Kuwabara, T. 2013 Anaerobic coculture of microalgae with Thermosipho globiformans and Methanocaldococcus jannaschii at 68 ºC enhances generarion of n-alkane-rich biofules after pyrolysis. Appl. Environ. Microbiol., 79, 924-930.
Strain(s): 8372258 
PubMed: 23183975
DOI: 10.1128/AEM.01685-12

Shimonaga, T., Konishi, M., Oyama, Y., Fujiwara, S., Satoh, A., Fujita, N., Colleoni, C., Buléon, A., Putaux, J.-L., Ball, S. G., Yokoyma, A., Hara, Y., Nakamura, Y., Tsuzuki, M. 2008 Variation in Storage α-polyglucans of the Porphyridiales (Rhodophyta). Plant Cell Physiol., 49, 103-116.
Keywords: Amylose; Floridean starch; Glycogen; Porphyridiales; Rhodophyta; Semi-amylopection
Strain(s): 181442505305512138216022582280 
PubMed: 18079144
DOI: 10.1093/pcp/pcm172

Suzuki, T., Imai, M., Ishida, M. 1997 Influence of environmental factors on phosphorous content in the marine microalga Dunaliella. Toxicol. Environ. Chem., 64, 173-181.
Keywords: Dunaliella; photosynthesis; polyphosphates; response surface model
Strain(s): 225422572258 
DOI: 10.1080/02772249709358547

Suzuki, T., Matsuo, T., Ohtaguchi, K., Koide, K. 1995 Gas-sparged bioreactors for CO2 fixation by Dunaliella tertiolecta. J. Chem. Tech. Biotechnol., 62, 351-358.
Strain(s): 2258 
DOI: 10.1002/jctb.280620407

Yongmanitchai, W., Ward, O. P. 1991 Screening of algae for potential alternative sources of eicosapentaenoic acid. Phytochemistry, 30, 2963-2967.
Keywords: algae; Thailand; w-3 fatty acids; linolenic acid; eicosapentaenoic acid; docosahexaenoic acid
Strain(s): 22562258 
DOI: 10.1016/S0031-9422(00)98231-1

Snell, T. W., Boyer, E. M. 1988 Thresholds for mictic female production in the rotifer Brachionus plicatilis (muller). J. Exp. Mar. Biol. Ecol., 124, 73-85.
Keywords: food level; mixis; population density threshold; reproduction; rotifer; sex
Strain(s): 2258 
DOI: 10.1016/0022-0981(88)90112-8

Belmans, D., Laere, A. V. 1988 Effect of ionophores on the ATP-pool and glycerol content in cells of the halotolerant green alga Dunaliella tertiolecta. J. Gen. Microbiol., 134, 2261-2268.
Strain(s): 2258 
DOI: 10.1099/00221287-134-8-2261

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