Strain number NIES-2169  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella sorokiniana Shihira & R.W.Krauss  
Former name Chlorella pyrenoidosa Chick  
Common name Green alga  
Locality (Date of collection) Waller Creek on Univ. TX Campus, Austin, Texas, USA  
Latitude / Longitude 30.259697 /  
Habitat (Isolation source) Freshwater  
History < IAM (2007) < Meyers, Jack  
Isolator (Date of isolation) Sorokin, C.  
Identified by Kessler, E.; CConfirmed by DNA sequencing at NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB080307 AB488573 ) , actin ( AB080311 )  
Cell size (min - max) 5 - 8 μm  
Organization Unicellular 
Characteristics Starch production (Takeshita et al. 2014)  
Other strain no. Other collection strain no. : IAM C-212; ATCC 22521; CAUP H1957; CCAP 211/8k; SAG 211-8K; UTEX 1230
Remarks Cryopreserved; Axenic 
Hirayama, A., Sueyoshi, M. N., Nakano, T., Ota, Y., Kurita, H., Tasaki, M., Kuroiwa, Y., Kato, T., Serizawa, S., Kojima, K., Al-Maamari, R. S., Hasegawa, T., Thomas-Hall, S. R., Schenk, P. M. 2022 .Development of large-scale microalgae production in the Middle East. Bioresour. Technol., 343, 126036 (article ID).
Keywords: Chlorella sorokiniana; Large-scale microalgae production; Outdoor raceway ponds; Photobioreactor; Water temperature control
Strain(s): 2169 
PubMed: 34626761
DOI: 10.1016/j.biortech.2021.126036

Tawfik, A., Niaz, H., Qadeer, K., Qyyum, M. A., Liu, J. J., Lee, M. 2022 Valorization of algal cells for biomass and bioenergy production from wastewater: Sustainable strategies, challenges, and techno-economic limitations. Renew. Sust. Energ. Rev., 157, 112024 (article ID).
Keywords: Xenobiotic compounds; Micro-pollutants; Enzyme activity; Biomass; Bioenergy; Algae-based wastewater treatment
Strain(s): 192344226422437216922203284 
DOI: 10.1016/j.rser.2021.112024

Anam, K., Rahman, D. Y., Hidhayati, N., Rachmayati, R., Susilaningsih, D., Agustini, N. W. S., Praharyawan, S., Susanti, H., Apriastini, M. 2021 Lipid accumulation on optimized condition through biomass production in green algae. IOP Conf. Ser.: Earth Environ. Sci., 762, 012075 (article ID).
Strain(s): 215221692170 
DOI: 10.1088/1755-1315/762/1/012075

Maltsev, Y., Maltseva, K., Kulikovskiy, M., Maltseva, S. 2021 Influence of light conditions on microalgae growth and content of lipids, carotenoids, and fatty acid composition. Biology-Basel, 10, 1060 (article ID).
Keywords: carotenoids; fatty acids; light; lipids; microalgae; photoperiod
Strain(s): 21512152215921692170 
PubMed: 34681157
DOI: 10.3390/biology10101060

Kato, T., Nakano, K., Sueyoshi, M., Tasaki, M., Kuroiwa, Y. 2020 Large-scale outdoor test of Chlorella culturing in raceway system in the Middle East. Shimizu Kensetsu Kenkyuhokoku, 97, 133-138 (in Japanese with English summary).
Strain(s): 2169 

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133.
Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS
Strain(s): 3514436241842143243845654557173273785810221048184821502169220122022208220922122280235224622578 
PubMed: 30304548
DOI: 10.1111/jpy.12798

Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134.
Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light
Strain(s): 215121522159216921702179 
PubMed: 24583913
DOI: 10.1016/j.biortech.2014.01.135

Mizuno, Y., Sato, A., Watanabe, K., Hirata, A., Takeshita, T., Ota, S., Sato, N., Zachleder, V., Tsuzuki, M., Kawano, S. 2013 Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species. Bioresour. Technol., 129, 150-155.
Keywords: Sulfur deficiency; Chlorella spp.; Parachlorella kessleri; Starch; Lipids
Strain(s): 21692173 
PubMed: 23238344
DOI: 10.1016/j.biortech.2012.11.030

Imase, M., Ohko, Y., Takeuchi, M., Hanada, S. 2013 Estimating the viability of Chlorella exposed to oxidative stresses based around photocatalysis. Int. Biodeterior. Biodegrad., 78, 1-6.
Keywords: Autofluorescence; Cell staining; Chlorella sorokiniana; Oxidative stress: TiO2 photocatalysis; Viability assay
Strain(s): 2169 
DOI: 10.1016/j.ibiod.2012.12.006

Chu, C. Y., Liao, W. R., Huang, R., Lin, L. P. 2004 Haemagglutinating and antibiotic activities of freshwater microalgae. World J. Microbiol. Biotechnol., 20, 817–825.
Keywords: Antibiotic activity; erythrocytes; freshwater microalgae; haemagglutination
Strain(s): 22622721592163216521692175218922082211221322142229223122332240224122432247224832773284 
DOI: 10.1007/s11274-004-8712-6

Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184.
Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection
Strain(s): 21602169217321864016 
DOI: 10.1046/j.1529-8817.2003.02052.

Kessler, E., Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553.
Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology
Strain(s): 6866872150215121602163216921752176 
DOI: 10.1111/j.0022-3646.1992.00550.x

Maruyama, I., Ando, Y., Maeda, T., Hirayama, K. 1989 Uptake of vitamin B12 by various strains of unicellular algae Chlorella. Nippon Suisan Gakkaishi, 55, 1785-1790.
Strain(s): 48226687215721692175 

Brand, J. J., Wright, J. N. 1989 Hydrogen production by eukaryotic algae. Biotech. Bioeng., 33, 1482-1488.
Strain(s): 2151216921902194220122022203220422112213221922792291 

Ohta, S., Miyamoto, K., Miura, Y. 1987 Hydrogen evolution as a consumption mode of reducing equivalents in green algal fermentation. Plant Physiol., 83, 1022-1026.
Strain(s): 21693284 
PubMed: 16665317
DOI: 10.1104/pp.83.4.1022

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