A big image opens when the thumbnail is clicked.(nies-0015.jpg)
Strain number NIES-15  
Phylum Heterokontophyta  
Class Olisthodiscophyceae  
Scientific name Olisthodiscus tomasii Barcytė, Eikrem & M.Eliáš  
Former name Olisthodiscus luteus N.Carter  
Common name  
Locality (Date of collection) Seto Inland Sea, Tamano, Okayama, Japan  
Latitude / Longitude  
Habitat (Isolation source) Marine (Coastal soil)  
History < Inouye, Isao  
Isolator (Date of isolation) Inouye, Isao  
Identified by Inouye, Isao  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  20 C
Light intensity:  6 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA , 18S-ITS1-5.8S-ITS2 , 28S rRNA , GapC1 , GapC2 , rbcL  
Cell size (min - max) - 19 μm  
Organization Unicellular; Flagellate 
Characteristics Red tide ; Authentic strain, Holotype (Barcytė et al. 2021)  
Other strain no. Other strain no. : Olisth  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie https://www.youtube.com/watch?v=gV_ikdEymk4&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW&index=19  
Barcytė, D., Eikrem, W., Engesmo, A., Seoane, S., Wohlmann, J., Horák, A., Yurchenko, T., Eliáš, M. 2021 Olisthodiscus represents a new class of Ochrophyta. J. Phycol., 57, 1094-1118.
Strain(s): 15 
PubMed: 33655496
DOI: 10.1111/jpy.13155

Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID).
Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles
Strain(s): 1571527433153459061569971290010171308131027642778 
DOI: 10.1016/j.algal.2021.102191

Engesmo, A., Strand, D., Gran-Stadniczeñko, S., Edvardsen, B., Medlin, LK., Eikrem, W. 2018 Development of a qPCR assay to detect and quantify ichthyotoxic flagellates along the Norwegian coast, and the first Norwegian record of Fibrocapsa japonica (Raphidophyceae). Harmful Algae, 75, 105-117.
Keywords: Karenia mikimotoi; Karlodinium veneficum; Heterosigma akashiwo; Molecular monitoring; rDNA; 454 high throughput environmental; sequencing; SEM
Strain(s): 15 
PubMed: 29778220
DOI: 10.1016/j.hal.2018.04.007

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1891415177111522322523323426527427527627727827928028128228429332332433033334434534634734835035337237738839139540740840941341441746146246648753454855355655755855956056258758858959060360562262369569669769869970070170270370470570670770870971071171271371471571674176576676780583799710011002100310041005100610071009101110161017104410451046104713021303132413271330133913401349135313701375137613791383138413851386138713911392139313951398139914001401169917001730181318151816182618271831186218631864186518741963196419651974197519762142214321442145214721482300233123322351236323642365236923702376250625332534253525362537259026332668268926902691269326942696269727072716271727182720272227232725272627292730273127322770277127722773283928402841284228432844285928722878289028993391368936903691 

Takishita, K., Yamaguchi, H., Maruyama, T., Inagaki, Y. 2009 A hypothesis for the evolution of nuclear-encoded, plastid-targeted glyceraldehyde-3 phosphate dehydrogenase genes in "chromalveolate" members. PLoS One, 4, e4737 (article ID).
Strain(s): 15275706716 
PubMed: 19270733
DOI: 10.1371/journal.pone.0004737

Hamana, K. 2008 Cellular polyamines of phototrophs and beterotrophs belonging to the lower eukaryotic phyla Cercozoa, Euglenozoa, Heterokonta and Metamonada. J. Gen. Appl. Microbiol., 54, 135-140.
Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine
Strain(s): 9157186118253323330333548603622100210031011104413031380144014431444 
PubMed: 18497488
DOI: 10.2323/jgam.54.135

Iida, K., Takishita, K., Ohshima, K., Inagaki, Y. 2007 Assessing the monophyly of chlorophyll-c containing plastids by multi-gene phylogenies under the unlinked model conditions. Mol. Phylogenet. Evol., 45(1), 227-238.
Keywords: chromist; chromalveolate; secondary endosymbiosis; model misspecification; separate model; concatenate model
Strain(s): 115548670716 
PubMed: 17591448
DOI: 10.1016/j.ympev.2007.05.003

Alamsjah, M. A., Hirao, S., Ishibashi, F., Fujita , Y. 2005 Isolation and structure determination of algicidal compounds from Ulva fasciata. Biosci. Biotechnol. Biochem., 69, 2186-2192.
Keywords: algicidal compounds; Ulva fasciata; hexadeca-4,7,10,13-tetraenoic acid; octadeca-6,9,12,15-tetraenoic acid; α-linolenic acid
Strain(s): 15462 
PubMed: 16306701
DOI: 10.1271/bbb.69.2186

Kuroda, A., Nakashima, T., Yamaguchi, K., Oda, T. 2005 Isolation and characterization of light-dependent hemolytic cytotoxin fromharmful red tide phytoplankton Chattonella marina. Comp. Biochem. Physiol. C-Pharmacol. Toxicol. Endocrinol., 141, 297-305.
Keywords: Red tide; Phytoplankton; Raphidophycean flagellate; Chattonella marina; Hemolysin; Photosensitizer; Pheophorbide a; Cytotoxin; Photodynamic therapy
Strain(s): 615 
PubMed: 16098818
DOI: 10.1016/j.cca.2005.07.009

Marshall, J. A., Nichols, P. D., Hallegraeff, G. M. 2002 Chemotaxonomic survey of sterols and fatty acids in six marine raphidophyte algae. J. Appl. Phycol., 14, 255-265.
Keywords: Chattonella antiqua; Chattonella marina; Chattonella subsalsa; chemotaxonomy; fatty acids; Fibrocapsa japonica; Heterosigma akashiwo; Olisthodiscus luteus; phycotoxins; raphydophytes; sterols
Strain(s): 15118 

Connell, L. 2002 Rapid identification of marine algae (Raphidophyceae) using three-primer peR amplification of nuclear internal transcribed spacer (ITS) regions from fresh and archived material. Phycologia, 41, 15-21.
Strain(s): 115 
DOI: 10.2216/i0031-8884-41-1-15.1

Kim, D., Okamoto, T., Oda, T., Tachibana, K., Lee, K. S., Ishimatsu, A., Matsuyama, Y., Honjo, T., Muramatsu, T. 2001 Possible involvement of the glycocalyx in the ichthyotoxicity of Chattonella marina (Raphidophyceae): immunological approach using antiserum against cell surface structures of the flagellate. Mar. Biol., 139, 625-632.
Strain(s): 615 
DOI: 10.1007/s002270100614

Oda, T., Sato, Y., Kim, D., Muramatsu, T., Matsuyama, Y., Honjo, T. 2001 Hemolytic activity of Heterocapsa circularisquama (Dinophyceae) and its possible involvement in shellfish toxicity. J. Phycol., 37, 509-516.
Keywords: Heterocapsa circularisquama; hemolysis; shellfish toxicity; harmful phytoplankton
Strain(s): 15 
DOI: 10.1046/j.1529-8817.2001.037004509.x

Kim, D., Nakamura, A., Okamoto, T., Komatsu, N., Oda, T., Ishimatsu, A., Muramatsu, T. 1999 Toxic potential of the raphidophyte Olisthodiscus luteus: mediation by reactive oxygen species. J. Plankton Res., 21, 1017-1027.
Strain(s): 15 
DOI: 10.1093/plankt/21.6.1017

Oda, T., Nakamura, A., Shikayama, M., Kawano,I., Ishimatsu, A., Muramatsu, T. 1997 Generation of reactive oxygen species by Raphidophycean phytoplankton. Biosci. Biotechnol. Biochem., 61, 1658-1662.
Keywords: red tide planlton; Raphidophyceae; Chattonella marina; reactive oxygen species; toxicity
Strain(s): 615 
PubMed: 9362113
DOI: 10.1271/bbb.61.1658

Yasumoto, T. 1992 Toxin of the marine micro-algae [Kaiyô bisaisôrui no Tokishin]. BIO Medica, 7, 57-62 (in Japanese).
Strain(s): 161215 

Nagai, H., Satake, M., Murata, M., Yasumoto, T. 1990 Screening of marine phytoplankton for antifungal substances. In Toxic Marine Phyto-plankton, Eds. by Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M., Elsevier Science Pub., New York, pp. 385-390.
Strain(s): 161215 

Uye, S. & Takamatsu, K. 1990 Feeding inter-actions between planktonic copepods and red-tide flagellates from Japanese coastal waters. Mar. Ecol. Prog. Ser., 59, 97-107.
Strain(s): 1215221235251 

Kawai, H., Inouye, I. 1989 Flagellar autofluorescence in forty-four chloraphyll c-containig algae. Phycologia, 28, 222-227.
Strain(s): 515161 
DOI: 10.2216/i0031-8884-28-2-222.1

Hara, Y., Inouye, I., Chihara, M. 1985 Morphology and ultrastructure of Olisthodiscus luteus (Raphidophyceae) with special reference to the taxonomy. Bot. Mag. Tokyo, 98, 251-262.
Keywords: Heterosigma; Olisthodiscus luteus; Raphidophyceae; red tide flagellate; taxonomy of Olisthodiscus; ultrastructure of Olisthodiscus
Strain(s): 15 
DOI: 10.1007/BF02488775

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