Strain number NIES-2095  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Anabaena variabilis Kützing ex Bornet & Flahault  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater  
History < IAM (2007) < Fujiwara, Shoko (1990)  
Isolator (Date of isolation)  
Identified by  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  MDM (agar)  
Temperature:  20 C
Light intensity:  9-12 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information Whole-genome ( CP000117 ) , 16S rRNA ( AB074502 ) , gyrB ( AB074766 ) , rpoC1 ( AB074789 ) , rpoD1 ( AB074816 )  
Cell size (min - max)  
Organization Filamentous 
Characteristics Genome decoded strain (Thiel et al. 2014)  
Other strain no. Other collection strain no. : IAM M-204; ATCC 29413
Remarks Axenic 
Tamamizu, K. & Kumazaki, S. 2019 Spectral microscopic imaging of heterocysts and vegetative cells in two filamentous cyanobacteria based on spontaneous Raman scattering and photoluminescence by 976 nm excitation. Biochim. Biophys. Acta-Bioenerg., 1860, 78-88.
Keywords: Heterocystous cyanobacteria; Raman scattering microscopy; Anabaena variabilis; Rivularia; Photosystems; Thylakoid membranes
Strain(s): 20952160 
PubMed: 30414930
DOI: 10.1016/j.bbabio.2018.11.012

Morsy, F. M., Elbadry, M., El-Sayed, W. S., Abd El-Hady, D. 2019 Dark and photofermentation H2 production from hydrolyzed biomass of the potent extracellular polysaccharides producing cyanobacterium Nostoc commune and intracellular polysaccharide (glycogen) enriched Anabaena variabil is NIES-2095. Int. J. Hydrog. Energy, 44, 16199-16211.
Keywords: Anabaena variabilis; Biohydrogen; Clostridium; Cyanobacterial polysaccharides; Nostoc commune; Rhodobacter capsulatus
Strain(s): 2095 
DOI: 10.1016/j.ijhydene.2019.05.024

Nozue, S., Katayama, M., Terazima, M., Kumazaki, S. 2017 Comparative study of thylakoid membranes in terminal heterocysts and vegetative cells from two cyanobacteria, Rivularia M-261 and Anabaena variabilis, by fluorescence and absorption spectral microscopy. Biochim. Biophys. Acta-Bioenerg., 1858, 742-749.
Keywords: Cyanobacteria; Heterocyst; Thylakoid membrane; Anabaena variabilis; Rivularia; Spectroscopic microscopy
Strain(s): 2095 
PubMed: 28576442
DOI: 10.1016/j.bbabio.2017.05.007

Nozue, S., Mukuno, A., Tsuda, Y., Shiina, T., Terazima, M., Kumazaki, S. 2016 Characterization of thylakoid membrane in a heterocystous cyanobacterium and green alga with dual-detector fluorescence lifetime imaging microscopy with a systematic change of incident laser power. Biochim. Biophys. Acta-Bioenerg., 1857, 46–59.
Keywords: Photosystem II; Photosystem I; Chlorophyll fluorescence; Fluorescence lifetime imaging microscopy; Heterocystous cyanobacteria; Green algae
Strain(s): 2095 
PubMed: 26474523
DOI: 10.1016/j.bbabio.2015.10.003

Thiel, T., Pratte, B. S., Zhong, J., Goodwin, L., Copeland, A., Lucas, S., Han, C., Pitluck, S., Land, M. L., Kyrpides, N. C., Woyke, T. 2014 Complete genome sequence of Anabaena variabilis ATCC 29413. Stand. Genomic Sci., 9, 562-573.
Keywords: Nitrogen Fixation; Joint Genome Institute; Heterotrophic Growth; Anabaena variabilis; Cyanobacterial Genome
Strain(s): 2095 
PubMed: 25197444
DOI: 10.4056/sigs.3899418

Kumazaki, S., Akari, M., Hasegawa, M. 2013 Transformation of thylakoid membranes during differentiation from vegetative cell into heterocyst visualized by microscopic spectral imaging. Plant Physiol., 161, 1321-1333.
Strain(s): 2095 
PubMed: 23274239
DOI: 10.1104/pp.112.206680

Makino, T., Harada, H., Ikenaga, H., Matsuda, S., Takaichi, S., Shindo, K., Sandmann, G., Ogata, T., Misawa, N. 2008 Characterization of cyanobacterial carotenoid ketolase CrtW and hydroxylase CrtR by complementation analysis in Escherichia coli. Plant Cell Physiol., 49, 1867-1878.
Keywords: Anabaena sp. PCC 7120; β-carotene; carotenoid; CrtR; CrtW; Nostoc punctiforme
Strain(s): 2095 
PubMed: 18987067
DOI: 10.1093/pcp/pcn169

Takaichi., S., Mochimaru, M., Maoka, T. 2006 Presense of free myxol and 4-hydroxymyxol and absense of myxol glycosides in Anabaena variabilis ATCC 29413, and proposal of a biosynthetic pathway of carotenoids. Plant Cell Physiol., 47, 211-216.
Keywords: Anabaena variabilis ATCC 29413; carotenogenesis; carotenoid; 4-hydroxymyxol; myxol
Strain(s): 2095 
PubMed: 16338959
DOI: 10.1093/pcp/pci236

Seo, P-S. & Yokota, A. 2003 The phylogenetic relationships of cyanobacteria inferred from 16S rRNA, gyrB, rpoC1 and rpoD1 gene sequences. J. Gen. Appl. Micobiol., 49, 191-203.
Keywords: cyanobacteria; gyrB; phylogeny; protein evolution; rpoC1; rpoD1; 16S rDNA
Strain(s): 19212225391042093209521283277 
PubMed: 12949700
DOI: 10.2323/jgam.49.191

Shiraiwa, Y. & Miyachi, S. 1985 Role of carbonic anhydrase in photosynthesis of blue-green alga (cyanobacterium) Anabaena variabilis ATCC 29413. Plant Cell Physiol., 26, 109-116.
Keywords: Anabaena variabilis ATCC 29413; carbonic anhydrase; CO2 effect; CO2 transport; cyanobacterium; photosynthesis
Strain(s): 2095 
DOI: 10.1093/oxfordjournals.pcp.a076877

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