Strain number NIES-4321  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Former name  
Common name Desmid  
Locality (Date of collection) Lake Inbanuma, Usuida, Sakura, Chiba, Japan (2016-06-25)  
Latitude / Longitude 35.741717 / 140.202283 
Habitat (Isolation source) Freshwater (Lake water)  
History < Tsuchikane, Yuki  
Isolator (Date of isolation) Tsuchikane, Yuki (2016-06-27)  
Identified by Tsuchikane, Yuki  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  20 C
Light intensity:  17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 1506 group I intron ( LC685248 )  
Cell size (min - max) 209 - 215 μm  
Organization Unicellular 
Characteristics Heterothallic ; Isogamous ; Mating group II-C ; Mating type (−) ; Crosses with NIES-4322 and NIES-261  
Other strain no. Other strain no. : Inba1-12  
Remarks Axenic 
Kawaguchi, Y. W., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2023 Extensive copy number variation explains genome size variation in the unicellular Zygnematophycean alga, Closterium peracerosum–strigosum–littorale complex. Genome Biol. Evol., 15, evad115 (article ID).
Keywords: gene duplication; dosage compensation; copy number variation; green algae
Strain(s): 53545859626364656826126664319432043214548455045514552 
PubMed: 37348049
DOI: 10.1093/gbe/evad115

Tsuchikane, Y., Hamaji, T., Ota, K., Kato, S. 2018 Establishment of a clonal culture of unicellular conjugating algae. J. Vis. Exp., 14, e57761 (article ID).
Strain(s): 4321 
PubMed: 30059030
DOI: 10.3791/57761

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