Strain number NIES-3715  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Chaetoceros tenuissimus Meunier  
Former name  
Common name Centric diatom  
Locality (Date of collection) off Maikohama, Harima-nada, Seto Inland Sea, Hyogo Japan (2002-08-10)  
Latitude / Longitude 34.62888888 / 135.03525 
Habitat (Isolation source) Marine (Seawater)  
History < Kimura, Kei < Tomaru, Yuji  
Isolator (Date of isolation) Tomaru, Yuji (2002-08-11)  
Identified by Suzuki, Hidekazu; Nagumo, Tamotsu  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  mSWM-3  
Temperature:  15 C
Light intensity:  9-11 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information Nuclear genome ( BLLK01000001-BLLK01000085 ) , Mitochondrial genome ( LC537470 ) , Plastid genome ( LC537471 ) , 18S rRNA ( AB847417 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Red tide ; Genome decoded strain (Hongo et al. 2021) ; The virus which infects with this diatom strain has been reported (Shirai et al. 2008).  
Other strain no. Other collection strain no. : CCAP 1010/43
Other strain no. : 2-10  
Remarks Cryopreserved; Axenic 
Hano, T. & Tomaru, Y. 2023 Chronological age-related metabolome responses in the dinoflagellate Karenia mikimotoi, can predict future bloom demise. Commun. Biol., 6, 273 (article ID).
Strain(s): 3715 
PubMed: 36922623
DOI: 10.1038/s42003-023-04646-z

Yamaguchi, H., Sumida, N., Matsukawa, K., Adachi, M., Tomaru, Y., Ishikawa, K., Hotta, J. 2023 Establishment of a simple method for cryopreservation of the marine diatoms, Chaetoceros and Phaeodactylum. J. Appl. Phycol., 33, 2285–2293.
Keywords: CELLBANKER; Cryopreservation; Marine diatoms; Chaetoceros tenuissimus; Phaeodactylum tricornutum; Transgenic diatom
Strain(s): 32437143715 
DOI: 10.1007/s10811-023-03040-w

Hongo, Y., Hano, T., Yamaguchi, H., Tamaru, Y. 2023 Transcriptional responses of the marine diatom Chaetoceros tenuissimus to phosphate deficiency. Gene, 884, 147695 (article ID).
Keywords: Phosphorus-deficiency; Differentially expressed genes; RNA-seq; Nonphospholipid; Alkaline phosphatase; Diatom
Strain(s): 3715 
PubMed: 37549856
DOI: 10.1016/j.gene.2023.147695

Grzebyk, D., Pasqualini, V., Garrido, M., Quilichini, Y., Pereto, C., Cecchi, P. 2022 Insight into the morphology and genetic diversity of the Chaetoceros tenuissimus (Bacillariophyta) species complex. Eur. J. Phycol., 57, 507-525.
Keywords: Cell division; Chaetoceros tenuissimus; internal transcribed spacer (ITS); morphology; phylogeny; rDNA; ultrastructure
Strain(s): 3715 
DOI: 10.1080/09670262.2022.2029949

Yamaguchi, H., Zaima, M., Adachi, M., Tomaru, Y., Asahara, H., Nishiwaki, N. 2022 Selective utilization of phosphorus compounds by Chaetoceros tenuissimus (Bacillariophyceae): Approach using 31P nuclear magnetic resonance analysis. Phycol. Res., 70, 151-159.
Strain(s): 3715 
DOI: 10.1111/pre.12485

Nef, C., Madoui, M., Pelletier, É., Bowler, C. 2022 Whole-genome scanning reveals environmental selection mechanisms that shape diversity in populations of the epipelagic diatom Chaetoceros. PLoS. Biol., 20, e3001893 (article ID).
Strain(s): 3715 
PubMed: 36441816
DOI: 10.1371/journal.pbio.3001893

Tomaru, Y., Yamaguchi, H., Miki, T. 2021 Growth rate-dependent cell death of diatoms due to viral infection and their subsequent coexistence in a semi-continuous culture system. Microbes Environ., 36, ME20116 (article ID).
Keywords: diatom; population dynamics; viral infection
Strain(s): 3715 
PubMed: 33390375
DOI: 10.1264/jsme2.ME20116

Hongo, Y., Kimura, K., Takaki, Y., Yoshida, Y., Baba, S., Kobayashi, G., Nagasaki, K., Hano, T., Tomaru, Y. 2021 The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus. Sci Rep, 11, 22877 (article ID).
Strain(s): 3715 
PubMed: 34819553
DOI: 10.1038/s41598-021-00565-3

Tomaru, Y. & Kimura, K. 2020 Novel protocol for estimating viruses specifically infecting the marine planktonic diatoms. Diversity-Basel, 12, 225 (article ID).
Keywords: marine diatom; Chaetoceros tenuissimus; Chaetoceros setoensis; host-virus specificity; RdRp; ssRNA virus; ssDNA virus; viral genome
Strain(s): 371237143715 
DOI: 10.3390/d12060225

Hano, T. & Tomaru, Y. 2019 Metabolomics-based approach to explore growth phase-dependent markers in cultured diatom Chaetoceros tenuissimus. J. Chromatogr. B, 1128, 121779 (article ID).
Keywords: Diatom; Metabolomics; Gas chromatography mass spectrometry; Liquid chromatography tandem mass spectrometry; Growth phase
Strain(s): 3715 
PubMed: 31499295
DOI: 10.1016/j.jchromb.2019.121779

Tomaru, Y., Toyoda, K., Kimura, K. 2018 Occurrence of the planktonic bloom-forming marine diatom Chaetoceros tenuissimus Meunier and its infectious viruses in western Japan. Hydrobiologia, 805, 221-230.
Keywords: Algal viruses; Primary producers; Marine algae; Real-time quantitative PCR; Restingstage cells
Strain(s): 37143715 
DOI: 10.1007/s10750-017-3306-0

Yamada, Y., Tomaru, Y., Fukuda, H., Nagata, T. 2018 Aggregate formation during the viral lysis of a marine diatom. Front. Mar. Sci., 15, 167 (article ID).
Keywords: aggregate; diatom–virus interaction; particle size distribution; settling velocity; material cycle
Strain(s): 3715 
DOI: 10.3389/fmars.2018.00167

Tomaru, Y., Shirai, Y., Toyoda, K., Nagasaki, K. 2011 Isolation and characterisation of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus. Aquat. Microb. Ecol., 64, 175-184.
Keywords: Bacilladnavirus; Chaetoceros; Diatom; ssDNA; Virus; Rod-shaped particle
Strain(s): 37143715 
DOI: 10.3354/ame01517

Toyoda, K., Nagasaki, K., Tomaru, Y. 2010 Application of real-time PCR assay for detection and quantification of bloom-forming diatom Chaetoceros tenuissimus Meunier. Plankton Benthos Res., 5, 56-61.
Keywords: Chaetoceros tenuissimus; detection; diatom; enumeration; TaqMan-based real-time PCR
Strain(s): 3715 
DOI: 10.3800/pbr.5.56

Shirai, Y., Tomaru, Y., Takao, Y., Suzuki, H., Nagumo, T., Nagasaki, K. 2008 Isolation and characterization of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus Meunier. Appl. Environ. Microbiol., 74, 4022-4027.
Strain(s): 3715 
PubMed: 18469125
DOI: 10.1128/AEM.00509-08

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