Strain number NIES-2239  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas reinhardtii P.A.Dangeard  
Synonym Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
Former name  
Common name Green alga  
Locality (Date of collection) near Amherst, Massachusetts, USA  
Latitude / Longitude  
Habitat (Isolation source) (Soil)  
History < IAM (2007) < Tsubo, Yoshihiro (1996) < Sager (1954) < Smith (1953)  
Isolator (Date of isolation)  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  2-3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information 18S rRNA ( AB701554 )  
Cell size (min - max) 8 - 10 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Mating type (+) ; Crosses with NIES-2235 (IAM C-9)  
Other strain no. Other collection strain no. : IAM C-562 (=C-8)
Other strain no. : Tsubo R (+)  
Remarks Cryopreserved; Axenic 
Nakanishi, A., Iritani, K., Sakihama, Y., Watanabe, M. 2020 Investigation of the mechanical strength of cell-plastics fabricated using unicellular green algal cells and varying weight ratios of biodegradable polybutylene succinate. Int. J. Microbiol. Biotechnol., 5, 159-164.
Keywords: Cell-plastics; Green Alga; Chlamydomonas reinhardtii; Polybutylene Succinate (PBS); Biodegradable
Strain(s): 2239 
DOI: 10.11648.j.ijmb.20200504.11

Livingston, G., Fukumori, K., Provete, D. B., Kawachi, M., Takamura, N., Leibold, M. A. 2017 Predators regulate prey species sorting and spatialdistribution in microbial landscapes. J. Anim. Ecol., 86, 501-510.
Keywords: bacteria; colonization; dispersal; disturbance; protists; spatial pattern; variation partitioning
Strain(s): 2239 
PubMed: 28138991
DOI: 10.1111/1365-2656.12639

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Hosokawa, Y., Miki-Noumura, T. 1987 Bending motion of Chlamydomonas axonemes after extrusion of central-pair microtubules. J. Cell Biol., 105, 1297-1301.
Strain(s): 2239 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

Tsubo, Y. & Matsuda, Y. 1984 Transmission of chloroplast genes in crosses between Chlamydomonas reinhardtii diploids: Correlation with chloroplast nucleoid behavior in young zygotes. Curr. Genet., 8, 223-229.
Keywords: Chlamydomonas; tetraploid; chloroplast gene transmission; DAPI staining
Strain(s): 22352239 
PubMed: 24177743
DOI: 10.1007/BF00417820

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