A big image opens when the thumbnail is clicked.(nies-2235.jpg)
Strain number NIES-2235  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas reinhardtii P.A.Dangeard  
Synonym Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
Former name  
Common name Green alga  
Locality (Date of collection) near Amherst, Massachusetts, USA  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Tsubo, Yoshihiro (1959) < Sager (1954) < Smith (1953)  
Isolator (Date of isolation)  
Identified by  
State of strain Subculture / Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  21 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information Total genome information ( http://chlamy.pmb.lif.kyoto-u.ac.jp/chlamybase ) , EST information ( http://est.kazusa.or.jp/en/plant/chlamy/EST/index.html ) , Amt protein mRNA(putative) ( AF509497 ) , CAH1 ( D90206 AB026126 ) , CAH2 ( X54488 ) , CrDES ( AB239769 ) , GCS1 ( AB206813 ) , mmp1 ( AB058411 AB058413 ) , mmp2 ( AB058412 AB058414 ) , mmp3 ( AB108850 ) , NSG1 ( AB167472 ) , NSG6 ( AB167474 ) , NSG7 ( AB167475 ) , NSG11 ( AB167476 ) , NSG13 ( AB167477 ) , NSG17 ( AB167478 ) , RECA ( AB048829 ) , SIG1 mRNA ( AB049220 ) , 18S rRNA ( AB701550 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Starch production (Kuchitsu et al. 1988) ; Genome decoded strain ; Mating type (−) ; Crosses with NIES-2239 (IAM C-562) ; Live food (Liu et al. 2016)  
Other strain no. Other collection strain no. : IAM C-9
Other strain no. : Tsubo R (−)  
Remarks Cryopreserved; Axenic 
Nakanishi, A., Yomogita, M., Horimoto, T. 2024 Evaluation of cellular responses by Chlamydomonas reinhardtii in media containing dairy-processing residues derived from cheese as nutrients by analyzing cell growth activity and comprehensive gene transcription levels. Microorganisms, 12, 715 (article ID).
Keywords: Chlamydomonas reinhardtii; whey; whey retentate; cellular response; transcriptomics; biomass; mineral; potassium
Strain(s): 2235 
PubMed: 38674659
DOI: 10.3390/microorganisms12040715

Nakanishi, A., Omino, N., Nakamura, T., Goto, S., Matsumoto, R., Yomogita, M., Narisawa, N., Kimijima, M., Iritani, K. 2024 Evaluation of cellular responses of heterotrophic Escherichia coli cultured with autotrophic Chlamydomonas reinhardtii as a nutrient source by analyses based on microbiology and transcriptome. Microorganisms, 12, 452 (article ID).
Keywords: Escherichia coli; Chlamydomonas reinhardtii; cellular response; transcriptomics
Strain(s): 2235 
PubMed: 38543503
DOI: 10.3390/microorganisms12030452

Mezgebu, A., Liu, X., Mingist, M., Ban, S. 2023 Evaluating food quality of Arthrospira platensis for culturing Daphnia magna. Aquac. Int., 32, 3533-3544.
Keywords: Arthrospira platensis; Daphnia magna; Essential biochemicals; Food quality
Strain(s): 392235 
DOI: 10.1007/s10499-023-01336-9

Nakanishi, A., Nemoto, S., Yamamoto, N., Iritani, K., Watanabe, M. 2023 Identification of cell-attachment factors derived from green algal cells disrupted by sonication in fabrication of cell plastics. Bioengineering-Basel, 10, 893 (article ID).
Keywords: cell plastics; unicellular green algae; attachment factors in matrices; proteomics
Strain(s): 2235 
PubMed: 37627778
DOI: 10.3390/bioengineering10080893

Nakanishi, A., Iritani, K., Tsuruta, A., Yamamoto, N., Watanabe, M., Ozawa, N., Watanabe, M., Zhang, K., Tokudome, A. 2022 Fabrication of cell plastics composed only of unicellular green alga Chlamydomonas reinhardtii as a raw material. Photosynth. Res., 106, 4459–4468.
Keywords: Biomass plastics; Cell plastics; Carbon recycling; Green algae; Chlamydomonas reinhardtii; Intracellular contents
Strain(s): 2235 
PubMed: 35676378
DOI: 10.1007/s00253-022-12000-2

Yamano, T., Toyokawa, C., Shimamura, D., Matsuoka, T., Fukuzawa, H. 2021 CO2-dependent migration and relocation of LCIB, a pyrenoid-peripheral protein in Chlamydomonas reinhardtii. Plant Physiol., 188, 1081–1094.
Strain(s): 2235 
PubMed: 34791500
DOI: 10.1093/plphys/kiab528

Nakanishi, A., Iritani, K., Sakihama, Y., Watanabe, M., Mochiduki, A., Tsuruta, A., Sakamoto, S., Ota, A. 2021 Fabrication and biodegradability of starch cell-plastics as recyclable resources. Appl. Sci., 11, 847 (article ID).
Keywords: cell-plastics; biodegradability; unicellular green alga; green plastics
Strain(s): 2235 
DOI: 10.3390/app11020847

Hang, L. T., Mori, K., Toyama, T. 2020 Enhanced biomass and lipid production capacity of Chlamydomonas reinhardtii under mixotrophic cultivation using sewage effluent and waste molasses. Jpn. J. Water Treat. Biol., 56, 57-66.
Keywords: Chlamydomonas reinhardtii; lipid production; mixotrophy; sewage effluent; organic waste
Strain(s): 2235 
DOI: 10.2521/jswtb.56.57

Nakanishi, A., Iritani, K., Sakihama, Y., Ozawa, N., Mochizuki, A., Watanabe, M. 2020 Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer. AMB Express, 10, 112 (article ID).
Keywords: Chlamydomonas reinhardtii; Two-dimensional polymer; Stacking structure; Cell-plastics
Strain(s): 2235 
PubMed: 32524300
DOI: 10.1186/s13568-020-01046-y

Hang, L. T., Mori, K., Tanaka, Y., Morikawa, M., Toyama, T. 2020 Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl2 stresses. Bioprocess. Biosyst. Eng., 43, 971–980.
Keywords: Chlamydomonas reinhardtii; NaCl; CaCl2; Lipid accumulation; Biofuel production
Strain(s): 2235 
PubMed: 32008095
DOI: 10.1007/s00449-020-02293-w

Yamano, T. & Fukuzawa, H. 2020 Transformation of the model microalga Chlamydomonas reinhardtii without cell-wall removal. In Electroporation Protocols. Methods in Molecular Biology, Eds. by Li, S., Chang, L., Teissie, J., Humana, New York, NY, 2050, pp. 155-161.
Keywords: Chlamydomonas reinhardtii; Electroporation; Photosynthesis; Protist; Square electric pulse
Strain(s): 2235 
PubMed: 31468489
DOI: 10.1007/978-1-4939-9740-4_16

Ishinishi, R., Matsuura, H., Tanaka, S., Nozawa, S., Tanada, K., Kawashita, N., Fujiyama, K., Miyasaka, H., Hirata, K. 2018 Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga. Gene, 640, 14-20.
Keywords: Stress response; Chlamydomonas reinhardtii; Secretory protein; Recombinant protein; Unknown function
Strain(s): 2235 
PubMed: 29017964
DOI: 10.1016/j.gene.2017.10.012

Hamada, M., Schröder, K., Bathia, J., Kürn, U., Fraune, S., Khalturina, M., Khalturin, K., Shinzato, C., Satoh, N., Bosch, T. C.G. 2018 Metabolic co-dependence drives the evolutionary ancient Hydra-Chlorella symbiosis. eLife, 7, e35122 (article ID).
Keywords: Chlorella; Hydra; evolutionary biology; genome; nitrogen metabolism; symbiosis
Strain(s): 216622352541 
PubMed: 29848439
DOI: 10.7554/eLife.35122

Toyama, T., Kasuya, M., Hanaoka, T., Kobayashi, N., Tanaka, Y., Inoue, D., Sei, K., Morikawa, M., Mori, K. 2018 Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater efuent. Biotechnol. Biofuels, 11, 176 (article ID).
Keywords: Microalgae; Wastewater efuent; Enhanced biomass production; Microalgae growth-promoting bacteria
Strain(s): 4821722235 
PubMed: 29983739
DOI: 10.1186/s13068-018-1174-0

Seto, K. & Degawa, Y. 2018 Collimyces mutans gen. et sp. nov. (Rhizophydiales, Collimycetaceae fam. nov.), a new chytrid parasite of Microglena (Volvocales, clade Monadinia). Protist, 169, 507-520.
Keywords: Chytrid; Collimyces mutans; Microglena; parasite; Rhizophydiales; zoospore ultrastructure
Strain(s): 7558582220223527432744 
PubMed: 29935342
DOI: 10.1016/j.protis.2018.02.006

Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M. 2018 Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N1-aminopentylspermidine, N8-aminopentylspermidine, and penta-amines.— Microb. Resour. Syst., 34, 73-82.
Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine
Strain(s): 1835242691735127112782185218722352261254025723983 

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 22022207221222352242 
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Liu, X., Syuhei, B. 2017 Effects of acclimatization on metabolic plasticity of Eodiaptomus japonicus (Copepoda: Calanoida) determined using an optical oxygen meter. J. Plankton Res., 39, 111-121.
Keywords: temperature acclimatization; metabolic rates; oxygen consumption rates; zooplankton; optical oxygen meter
Strain(s): 2822235 
DOI: 10.1093/plankt/fbw084

Kong, F., Yamasaki, T., Kurniasih, S. D., Hou, L., Li, X., Ivanova, N., Okada, S., Ohama, T. 2015 Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains. Biotech. Bioeng., 120, 239-245.
Keywords: Transgene silencing; Position effect; Squalene synthase-like gene; Botryococcus braunii; Methyltransferase gene
Strain(s): 2235 
PubMed: 25660568
DOI: 10.1016/j.jbiosc.2015.01.005

Liu, X., Beyrend-Dur, D., Dur, G., Ban, S. 2015 Combined effects of temperature and food concentration on growth and reproduction of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan). Freshw. Biol., 60, 2003-2018.
Keywords: copepod dynamics; egg production; Eodiaptomus japonicus; individual growth rates; temperature–food effects
Strain(s): 2822235 
DOI: 10.1111/fwb.12626

Liu, X., Beyrend-Dur, D., Dur, G., Ban, S. 2014 Effects of temperature on life history traits of Eodiaptomus japonicus (Copepoda: Calanoida) from Lake Biwa (Japan). Limnology, 15, 85-97.
Keywords: Life history traits; Temperature effects; Eodiaptomus japonicus; Copepod dynamics; Individual variability
Strain(s): 2822235 
DOI: 10.1007/s10201-013-0417-9

Wang, L., Yamano, T., Kajikawa, M., Hirono, M., Fukuzawa, H. 2014 Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii. Photosynth. Res., 121, 175-184.
Keywords: Chlamydomonas reinhardtii; Carbon-concentrating mechanism; CO2-signal transduction; High-CO2-requiring mutants
Strain(s): 2235 
PubMed: 24549931
DOI: 10.1007/s11120-014-9983-x

Yamano, T., Iguchi, H. Fukuzawa, H. 2013 Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal. J. Biosci. Bioeng., 115, 691-694.
Keywords: Chlamydomonas reinhardtii; Electroporation; Square electric pulse; Transformation
Strain(s): 2235 
PubMed: 23333644
DOI: 10.1016/j.jbiosc.2012.12.020

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Bioresour. Technol., 109, 300-303.
Keywords: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate
Strain(s): 351192272302235 
PubMed: 21906932
DOI: 10.1016/j.biortech.2011.07.101

Fujii, K., Nakashima, H., Hashidzume, Y. 2010 Isolation of folate-producing microalgae, from oligotrophic ponds in Yamaguchi, Japan. J. Appl. Microbiol., 108, 1421-1429.
Keywords: B-vitamins; Chlamydomonas; Chlorococcum; folate; microalgae; thiamine
Strain(s): 217222352848284928502851 
PubMed: 19796121
DOI: 10.1111/j.1365-2672.2009.04542.x

Yamano, T., Miura, K., Fukuzawa, H. 2008 Expression analysis of genes associated with the induction of the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol., 147, 340-354.
Strain(s): 2235 
PubMed: 18322145
DOI: 10.1104/pp.107.114652

Kajikawa, M., Yamato, K. T., Kohzu, Y., Shoji, S., Sakai, Y., Fukuzawa, H. 2006 A front-end desaturase from the green alga Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by ω13 desaturation in methylotrophic yeast and tobacco. Plant Cell Physiol., 47, 64-73.
Keywords: Chlamydomonas reinhardtii; Δ5-unsaturated bis-methylene-interrupted fatty acids; fatty acid desaturase; Pichia pastoris; tobacco plant
Strain(s): 2235 
PubMed: 16267098
DOI: 10.1093/pcp/pci224

Abe, J., Kubo, T., Takagi, Y., Saito, T., Miura, K., Fukuzawa, H., Matsuda, Y. 2004 The transcriptional program of synchronous gametogenesis in Chlamydomonas reinhardtii. Curr. Genet., 46, 304-315.
Strain(s): 2235 
PubMed: 15459796
DOI: 10.1007/s00294-004-0526-4

Asamizu, E., Nakamura, Y., Miura, K., Fukuzawa, H., Fujiwara, S., Hirono, M., Iwamoto, K., Matsuda, Y., Minagawa, J., Shimogawara, K., Takahashi, Y., Tabata, S. 2004 Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta), to facilitate gene function analysis. Phycologia, 43, 722-726.
Strain(s): 2235 
DOI: 10.2216/i0031-8884-43-6-722.1

Miura, K., Yamano, T., Yoshioka, S., Kohinata, T., Inoue, Y., Taniguchi, F., Asamizu, E., Nakamura, Y., Tabata, S., Yamato, K. T., Ohyama, K., Fukuzawa, H. 2004 Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol., 135, 1595-1607.
Strain(s): 2235 
PubMed: 15235119
DOI: 10.1104/pp.104.041400

Yoshioka, S., Taniguchi, F., Miura, K., Inoue, T., Yamano, T., Fukuzawa, H. 2004 The novel Myb transcription factor LCR1 regulates the CO2-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii. Plant Cell, 16, 1466-1477.
Strain(s): 2235 
PubMed: 15155888
DOI: 10.1105/tpc.021162

Kubo, T., Abe, J.,  Saito, T., Matsuda, Y. 2002 Genealogical relationships among laboratory strains of Chlamydomonas reinhardtii as inferred from matrix metalloprotease genes. Curr. Genet., 41, 115-122.
Keywords: Chlamydomonas reinhardtii; Chlamydomonas smithii; matrix metalloprotease genes; molecular phylogeny
Strain(s): 2235 
PubMed: 12073093
DOI: 10.1007/s00294-002-0284-0

Kubo, T., Saito, T., Fukuzawa, H., Matsuda, Y. 2001 Two tandemly-located matrix metalloprotease genes with different expression patterns in the Chlamydomonas sexual cell cycle. Curr. Genet., 40, 136-143.
Keywords: cell wall degradation; Chlamydomonas reinhardtii; gametolysin; zygote-specific gene
Strain(s): 2235 
PubMed: 11680823
DOI: 10.1007/s002940100239

Asamizu, E., Miura, K., Kucho, K., Inoue, Y., Fukuzawa, H., Ohyama, K., Nakamura, Y., Tabata, S. 2000 Generation of expressed sequence tags from low-CO2 and high-CO2 adapted cells of Chlamydomonas reinhardtii. DNA Res., 7, 305-307.
Keywords: Chlamydomonas reinhardtii; cDNA; EST; CO2 stress
Strain(s): 2235 
PubMed: 11089912
DOI: 10.1093/dnares/7.5.305

Asamizu, E., Nakamura, Y., Sato, S., Fukuzawa, H., Tabata, S. 1999 A large scale structure analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res., 6, 369-373.
Keywords: green alga; Chlamydomonas reinhardtii; cDNA; EST; normalized library; similarity search
Strain(s): 2235 
PubMed: 10691129
DOI: 10.1093/dnares/6.6.369

Ban, S. 1992 Effects of photoperiod, temperature, and population density on induction of diapause egg production in Eurytemora affinis (Copepoda: Calanoida) in Lake Ohnuma, Hokkaido, Japan. J. Crustacean Biol., 12, 361-367.
Keywords: Eggs; Diapause; Female animals; Photoperiod; Nauplii; Egg production; Lakes; Population density; Autumn; Animals
Strain(s): 2822235 
DOI: 10.2307/1549029

Tachiki, A., Fukuzawa, H., Miyachi, S. 1992 Characterization of carbonic anhydrase isozyme CA2, which is the CAH2 gene product, in Chlamydomonas reinhardtii. Biosci. Biotechnol. Biochem., 56, 794-798.
Strain(s): 2235 
PubMed: 1368343
DOI: 10.1271/bbb.56.794

Fukuzawa, H., Ishida, S., Miyachi, S. 1991 cDNA cloning and gene expression of carbonic anhydrase in Chlamydomonas reinhardtii. Can. J. Bot., 69, 1088-1096.
Keywords: photosynthesis; light regulation; zinc; CO2-concentrating mechanism; intracellular processing
Strain(s): 2235 
DOI: 10.1139/b91-139

Kamo, T., Shimogawara, K., Fukuzawa, H., Muto, S., Miyachi, S. 1990 Subunit constitution of carbonic anhydrase from Chlamydomonas reinhardtii. Eur. J. Biochem., 192, 557-562.
Strain(s): 2235 
PubMed: 2120056
DOI: 10.1111/j.1432-1033.1990.tb19261.x

Kashino, Y., Enami, I., Satoh, K., Katoh, S. 1990 Immunological cross-reactivity among corresponding proteins of photosystems I and II from widely divergent photosynthetic organisms. Plant Cell Physiol., 31, 479-488.
Keywords: antiserum; cross-reactivity; immunoblotting; photosystem; protein
Strain(s): 209321382235 
DOI: 10.1093/oxfordjournals.pcp.a077935

Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., Kawaguchi, A. 1990 Effects of CO2 concentration during growth on fatty acid composition in microalgae. Plant Physiol., 93, 851-856.
Strain(s): 482093213821602235 
PubMed: 1666759
DOI: 10.1104/pp.93.3.851

Fukuzawa, H., Fujiwara, S., Yamamoto, Y., Dionisio-Sese, M. L., Miyachi, S. 1990 cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: Regulation by environmental CO2 concentration. Proc. Nati. Acad. Sci. U. S. A., 87, 4383-4387.
Strain(s): 2235 
PubMed: 2112252
DOI: 10.1073/pnas.87.11.4383

Ehara, T., Ogasawara, Y., Osafune, T. 1990 Behavior of chloroplast nucleoids during the cell cycle of Chlamydomonas reinhardtii (Chlorophyta) in synchronized culture. J. Phycol., 26, 317-323.
Keywords: cell cycle; Chlamydomons; chloroplast division; DAPI; nucleoid
Strain(s): 2235 
DOI: 10.1111/j.0022-3646.1990.00317.x

Dionisio-Sese, M. L., Fukuzawa, H., Miyachi, S. 1990 Light-induced carbonic anhydrase expression in Chlamydomonas reinhardtii. Plant Physiol., 94, 1103-1110.
Strain(s): 2235 
PubMed: 16667803
DOI: 10.1104/pp.94.3.1103

Fujiwara, S., Fukuzawa, H., Tachiki, A., Miyachi, S. 1990 Structure and differential expression of two genes encoding carbonic anhydrase in Chlamydomonas reinhardtii. Proc. Nati. Acad. Sci. U. S. A., 87, 9779-9783.
Strain(s): 2235 
PubMed: 2124702
DOI: 10.1073/pnas.87.24.9779

Dionisio, M. L., Tsuzuki, M., Miyachi, S. 1989 Light requirement for carbonic anhydrase induction in Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 207-213.
Keywords: blue light; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; light intensity
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a077731

Dionisio, M. L., Tsuzuki, M., Miyachi, S. 1989 Blue light induction of carbonic anhydrase activity in Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 215-219.
Keywords: blue light; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; photoreceptor
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a077732

Toguri, T., Muto, S., Mihara, S., Miyachi, S. 1989 Synthesis and degradation of carbonic anhydrase in a synchronized culture of Chlamydomonas reinhardtii. Plant Cell Physiol., 30, 533-539.
Keywords: carbonic anhydrase synthesis; Chlamydomonas reinhardtii; synchronous culture
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a077772

Tsuzuki, M. & Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104.
Strain(s): 20932138216021732235 
DOI: 10.1016/0304-3770(89)90051-X

Kuchitsu, K., Tsuzuki, M., Miyachi, S. 1988 Changes of starch localization within the chloroplast induced by changes in CO2 concentration during growth of Chlamydomonas reinhardtii. Independent regulation of pyrenoid starch and stroma starch. Plant Cell Physiol., 29, 1269-1278.
Keywords: Chlamydomonas (Chlorophyta); chloroplast; CO2 concentration; pyrenoid; starch; stroma
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a077635

Kuchitsu, K., Tsuzuki, M., Miyachi, S. 1988 Characterization of the pyrenoid isolated from unicellular green alga Chlamydomonas reinhardtii. Particulate form of RuBisCO protein. Protoplasma, 144, 17-24.
Keywords: Chlamydomonas; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO); stroma
Strain(s): 2235 
DOI: 10.1007/BF01320276

Bürger, J., Miyachi, S., Galland, P., Senger, H. 1988 Quantum requirements of photosynthetic oxygen evolution and 77K fluorescence emission spectra in unicellular green algae grown under low- and high-CO2-conditions. Botanica Acta, 101, 229-232.
Strain(s): 21732235 
DOI: 10.1111/j.1438-8677.1988.tb00037.x

Yagawa, Y., Muto, S., Miyachi, S. 1988 Carbonic anhydrase of Chlamydomonas reinhardtii. Cellular distribution and subunit constitution in two strains. Plant Cell Physiol., 29, 185-188.
Keywords: carbonic anhydrase (cellular distribution); Chlamydomonas
Strain(s): 2235 

Toguri, T., Muto, S., Miyachi, S. 1986 Biosynthesis and intracellular processing of carbonic anhydrase in Chlamydomonas reinhardtii. Eur. J. Biochem., 58, 443-450.
Strain(s): 2235 

Yagawa, Y., Aizawa, K., Yang, S.-Y., Miyachi, S. 1986 Release of carbonic anhydrase from the cell surface of Chlamydomonas reinhardtii by trypsin. Plant Cell Physiol., 27, 215-221.
Keywords: carbonic anhydrase; cell surface; Chlamydomonas reinhardtii; trypsin treatment
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a077093

Aizawa, K. & Miyachi, S. 1986 Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Lett., 39, 215-233.
Keywords: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport
Strain(s): 2093216021732235 
DOI: 10.1111/j.1574-6968.1986.tb01860.x

Yang, S.-Y., Tsuzuki, M., Miyachi, S. 1985 Carbonic anhydrase of Chlamydomonas: Pulification and studies on its induction using antiserum against Chlamydomonas carbonic anhydrase. Plant Cell Physiol., 26, 25-34.
Keywords: affinity chromatography; carbon dioxide concentration; carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a076892

Miyachi, S., Tsuzuki, M., Aizawa, K. 1985 Factors which enhance photosynthesis in microalgae under limiting CO2 concentrations. In Kinetics of Photosynthetic Carbon Metabolism in C3-plants. II. Electron Transport, Transport of CO2, Mathematical Models, Eds. by Viil, J. et al., Akademiya Nauka, Estonia SSR, pp. 88-92.
Strain(s): 21602235 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 6872160216121622170217322352280 
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Tsubo, Y. & Matsuda, Y. 1984 Transmission of chloroplast genes in crosses between Chlamydomonas reinhardtii diploids: Correlation with chloroplast nucleoid behavior in young zygotes. Curr. Genet., 8, 223-229.
Keywords: Chlamydomonas; tetraploid; chloroplast gene transmission; DAPI staining
Strain(s): 22352239 
PubMed: 24177743
DOI: 10.1007/BF00417820

Tsuzuki, M. 1983 Mode of HCO3-utilization by the cells of Chlamydomonas reinhardtii grown under ordinary air. Z. Pflanzenphysiol., 110, 29-37.
Strain(s): 216021702235 

Mihara, S. & Hase, E. 1978 Studies on the vegetative life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. V. The transition point for the effect of 6-methyl purine in the cell cycle. Plant Cell Physiol., 19, 83-89.
Keywords: cell cycle; Chlamydomonas; 6-methyl purine; transition point
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a075582

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1976 Electron microscope studies of the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. IV. Mitochondria in dividing cells. J. Electr. Microsc., 25, 261-269.
Strain(s): 2235 

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1975 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. III. Three-dimensional structures of mitochondria in the cells at intermediate stages of the growth phase of the cell cycle. J. Electr. Microsc., 24, 247-252.
Strain(s): 2235 

Mihara, S. & Hase, E. 1975 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. III. Some notes on the process of zoospore liberation. Plant Cell Physiol., 16, 371-375.
Strain(s): 2235 

Mihara, S. & Hase, E. 1975 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. IV. Effects of 6-methyl purine on the revolution of the cell cycle. Plant Cell Physiol., 16, 1121-1130.
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a075231

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1972 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. II. Association of mitochondria and the chloroplast at an early developmental stage. Plant Cell Physiol., 13, 981-989.
Strain(s): 2235 

Osafune, T., Mihara, S., Hase, E., Ohkuro, I. 1972 Electron microscope studies on the vegetative cellular life cycle of Chlamydomonas reinhardi Dangeard in synchronous culture. I. Some characteristics of changes in subcellular structures during the cell cycle, especially in formation of giant mitochondria. Plant Cell Physiol., 13, 211-227.
Strain(s): 2235 
DOI: 10.1093/oxfordjournals.pcp.a074732

Mihara, S. & Hase, E. 1971 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. I. Some characteristics of the cell cycle. Plant Cell Physiol., 12, 225-236.
Strain(s): 2235 

Mihara, S. & Hase, E. 1971 Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture. II. Effects of chloramphenicol and cycloheximide on the length of cell cycle. Plant Cell Physiol., 12, 237-241.
Strain(s): 2235 

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