Strain number NIES-4321  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Former name  
Common name Desmid  
Locality (Date of collection) Lake Inbanuma, Usuida, Sakura, Chiba, Japan (2016-06-25)  
Latitude / Longitude 35.741717 / 140.202283 
Habitat (Isolation source) Freshwater (Lake water)  
History < Tsuchikane, Yuki  
Isolator (Date of isolation) Tsuchikane, Yuki (2016-06-27)  
Identified by Tsuchikane, Yuki  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  20 C
Light intensity:  17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 1506 group I intron ( LC685248 )  
Cell size (min - max) 209 - 215 μm  
Organization Unicellular 
Characteristics Heterothallic ; Isogamous ; Mating group II-C ; Mating type (−) ; Crosses with NIES-4322 and NIES-261  
Other strain no. Other strain no. : Inba1-12  
Remarks Axenic 
Tsuchikane, Y., Hamaji, T., Ota, K., Kato, S. 2018 Establishment of a clonal culture of unicellular conjugating algae. J. Vis. Exp., 14, e57761 (article ID).
Strain(s): 4321 
PubMed: 30059030
DOI: 10.3791/57761

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