Strain number NIES-3581  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Nitzschia sp.  
Former name  
Common name Pennate diatom  
Locality (Date of collection) Funaura, Taketomi, Okinawa, Japan (2012-02-04)  
Latitude / Longitude  
Habitat (Isolation source) Brackish water (Plant (Mangrove))  
History < Nakayama, Takeshi < Kamikawa, Ryoma  
Isolator (Date of isolation) Kamikawa, Ryoma (2012-02-11)  
Identified by Mayama, Shigeki  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  ESM + CB-V  
Temperature:  20 C
Light intensity:  0 µmol photons/m2/sec,
Duration:  14 D  
Gene information Mitochondrial DNA ( AP018510 ) , 28S rRNA ( AB899689 ) , 16S rRNA ( AB899709 )  
Cell size (min - max) 10 - μm  
Organization Unicellular 
Characteristics Heterotrophic ; Benthic ; Nonphotosynthetic plastids ; Genome decoded strain (Kamikawa et al. 2018)  
Other strain no. Other strain no. : IriIs04  
Remarks Cryopreserved; Axenic; Nonphotosynthetic plastids 
Onyshchenko, A., Roberts, W. R., Ruck, E. C., Lewis, J. A., Alverson, A. J. 2021 The genome of a nonphotosynthetic diatom provides insights into the metabolic shift to heterotrophy and constraints on the loss of photosynthesis. New Phytol., 232, 1750-1764.
Keywords: apochloritic; B-ketoadipate pathway; Entner-Doudoroff Pathway; glycolysis; lignin; mixotrophy; oxidative pentose phosphate pathway; photosynthesis
Strain(s): 3581 
PubMed: 34379807
DOI: 10.1111/nph.17673

Moog, D., Nozawa, A., Tozawa, Y., Kamikawa, R. 2020 Substrate specifcity of plastid phosphate transporters in a non-photosynthetic diatom and its implication in evolution of red algaderived complex plastids. Sci Rep, 10, 1167 (article ID).
Strain(s): 3581 
PubMed: 31980711
DOI: 10.1038/s41598-020-58082-8

Onyshchenko, A., Ruck, E. C., Nakov, T., Alverson, A. J. 2019 A single loss of photosynthesis in the diatom order Bacillariales (Bacillariophyta). Am. J. Bot., 106, 560-572.
Keywords: Nitzschia; apochloritic; chloroplast; photosynthesis; phylogeny; plastid
Strain(s): 3581 
PubMed: 30958893
DOI: 10.1002/ajb2.1267

Kamikawa, R., Azuma, T., Ishii, K-I., Matsuno, Y., Miyashita, H. 2018 Diversity of organellar genomes in non-photosynthetic diatoms. Protist, 169, 351-361.
Keywords: ATP synthase complex; mitochondria; Nitzschia; plastids; vitamin B1
Strain(s): 272935763581 
PubMed: 29803116
DOI: 10.1016/j.protis.2018.04.009

Kamikawa, R., Yubuki, N., Yoshida, M., Taira, M., Nakamura, N., Ishida, K-I., Leander, B. S., Miyashita, H., Hashimoto, T., Mayama, S., Inagaki, Y. 2015 Multiple losses of photosynthesis in Nitzschia (Bacillariophyceae). Phycol. Res., 63, 19-28.
Keywords: apochlorotic diatoms; genetic diversity; large subunit rRNA; molecular phylogenetic analysis; nonphotosynthetic plastids; plastid 16S rRNA
Strain(s): 357635773578357935803581 
DOI: 10.1111/pre.12072

Kamikawa, R., Tanifuji, Ishikawa, G. S., Ishii, K., Matsuno, Y., Onodera, N. T., Ishida, K-I., Hashimoto, T., Miyashita, H., Mayama, S., Inagaki, Y. 2015 Proposal of a Twin-arginine translocator system-mediated constraint against loss of ATP synthase genes from nonphotosynthetic plastid genomes. Mol. Biol. Evol., 32, 2598-2604.
Keywords: apochlorotic diatoms; nonphotosynthetic plastid genome; genome reduction; ATP synthase complex; Twin-arginine translocator
Strain(s): 3581 
PubMed: 26048548
DOI: 10.1093/molbev/msv134

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