Strain Data

A big image opens when the thumbnail is clicked.(nies-0512.jpg)

Strain number		NIES-512
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Pseudanabaena foetida Niiyama, Tuji & Ichise var. intermedia Tuji & Niiyama
Synonym		Phormidium tenue Gomont; Pseudanabaena galeata Böcher
Former name		Formerly identified as Pseudanabaena galeata, re-identified by Tuji & Niiyama 2016[2016 Oct]; Phormidium tenue
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Nagoya Castle, Nagoya, Aichi, Japan (1981-11-01)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (Pond water)
History		< Yamada, Naoki
Isolator (Date of isolation)		Yamada, Naoki (1985-05-22)
Identified by		Tuji, Akihiro; Niiyama, Yuko
State of strain		Subculture / Cryopreservation; Unialgal; Non-clonal; Non-axenic
Culture condition (Preculture condition)		Medium: CT Temperature: 20 (20) C Light intensity: 21 (13-21) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 20 D (7 D)
Gene information		16S rRNA ( AB042838 LC153790 LC314126 ) , rbcL ( LC314127 ) , pgmtc ( AB826230 ) , pgmtf ( AB826229 )
Cell size (min - max)		2 - 8 μm
Organization		Filamentous
Characteristics		Offensive odor ; Test strain (Nagai, T., 2016) ; 2-MIB production (Iwase et al. 2010) ; Type strain (Tuji & Niiyama 2016)
Other strain no.		Other strain no. : PM-81A
Remarks		Cryopreserved; Unstable
Movie

Reference
Dayarathne, K., Ishikawa, T., Kadeer, A., Yamaguchi, M., Kawai-Yamada, M. 2024 The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia. , Arch. Microbiol., 367 (article ID). Keywords: Cyanobacteria; 2-MIB; Gene expression; Light-dependent Strain(s): 512 DOI: 10.1007/s00203-024-04099-w Senavirathna, M. D. H. J. & Jayasekara, M. A. D. D. 2023 Temporal variation of 2-MIB and geosmin production by Pseudanabaena galeata and Phormidium ambiguum exposed to high-intensity light. Water Environ. Res., 95, e10834 (article ID). Keywords: extreme light; filamentous cyanobacteria; growth suppression; odorous compounds; stress response Strain(s): 512, 2119 PubMed: 36635233 DOI: 10.1002/wer.10834 Christodoulou, M., Wahlsten, M., Sivonen, K. 2023 Morphological and Molecular Evaluation of Pseudanabaena epilithica sp. nov. and P. suomiensis sp. nov. (Pseudanabaenaceae, Cyanobacteria) from Finland. Diversity-Basel, 15, 909 (article ID). Keywords: Pseudanabaena; cyanobacteria; polyphasic approach; 16S rRNA gene; 16S–23S ITS; Finland Strain(s): 512, 4062, 4063, 4064, 4065, 4237, 4238 DOI: 10.3390/d15080909 Lee, J. E., Yu, M. N., Yu, S., Byeon, M. 2022 Occurrence and phylogenetic analysis of Pseudanabaena sp. producing 2-methylisoborneol in drinking water source of South Korea. Environ. Microbiol. Rep., 14, 197-202. Strain(s): 512 PubMed: 34837342 DOI: 10.1111/1758-2229.13031 Suruzzaman, M., Cao, T., Lu, J., Wang, Y., Su, M., Yang, M. 2022 Evaluation of the MIB-producing potential based on real-time qPCR in drinking water reservoirs. Environ. Res., 204, 112308 (article ID). Keywords: 2-Methylisoborneol (MIB); Taste & odor; Real time qPCR; MIB-Synthesis (mic) gene; Drinking water Strain(s): 512, 4062, 4063, 4237, 4238 PubMed: 34757030 DOI: 10.1016/j.envres.2021.112308 Shen, O., Wang, Q., Miao, H., Shimada, M., Utsumi, M., Lei, Z., Zhang, Z., Nishimura, O., Asada, Y., Fujimoto, N., Takanashi, H., Akiba, M., Shimizu, K. 2021 Temperature affects growth, geosmin/2-methylisoborneol production, and gene expression in two cyanobacterial species. Environ. Sci. Pollut. Res., 29, 12017–12026. Keywords: Cyanobacteria; Temperature; Cell growth; Geosmin; 2-MIB; Gene expression Strain(s): 512, 824 PubMed: 34558048 DOI: 10.1007/s11356-021-16593-5 Devi, A., Chiu, Y.-T., Hsueh, H.-T., Lin, T.-F. 2021 Quantitative PCR based detection system for cyanobacterial geosmin/2-methylisoborneol (2-MIB) events in drinking water sources: Current status and challenges. Water Res., 188, 116478 (article ID). Keywords: biomolecular; drinking water; database; geo; mic; monitoring; Taq-Man probes Strain(s): 512, 4062, 4063, 4237, 4238 PubMed: 33045635 DOI: 10.1016/j.watres.2020.116478 Kim, K., Yoon, Y., Cho, H., Hwang, S. J 2020 Molecular probes to evaluate the synthesis and production potential of an odorous compound (2-methylisoborneol) in cyanobacteria. Int. J. Environ. Res. Public Health, 17, 1933 (article ID). Keywords: 2-Methylisoborneol (2-MIB); cyanobacteria; molecular probes; mibC; real-time PCR Strain(s): 19, 512, 824 PubMed: 32188031 DOI: 10.3390/ijerph17061933 Muhetaer, G., Asaeda, T., Jayasanka, S. M. D. H., Baniya, M. B., Abeynayaka, H. D. L., Rashid, M. H., Yan, H. Y. 2020 Effects of light intensity and exposure period on the growth and stress responses of two cyanobacteria species: Pseudanabaena galeata and Microcystis aeruginosa. Water, 12, 407 (article ID). Keywords: cyanobacterial growth; stress responses; Pseudanabaena galeata; Microcystis aeruginosa; oxidative stress; antioxidative enzymes Strain(s): 111, 512 PubMed: 32045866 DOI: 10.3390/w12020407 Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578. Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757 PubMed: 32501712 DOI: 10.1021/jasms.0c00148 Oliveira, E. D. C. de, Cunha, A. C. da, Silva, N. B. da, Castelo-Branco, R., Morais, J., Schneider, M. P. C., Faustino, S. M. M., Ramos, V., Vasconcelos, V. 2019 Morphological and molecular characterization of cyanobacterial isolates from the mouth of the Amazon River. Phytotaxa, 387, 269-288. Keywords: Amazonia; Limnothrix; Polyphasic approach; Pseudanabaena; 16S rRNA phylogeny; Algae Strain(s): 504, 512 DOI: 10.11646/phytotaxa.387.4.1 Strain(s): 75, 333, 512, 805, 1642, 1666, 1677, 1696, 1723 DOI: 10.34566/jwwa.88.12_3 Tuji, A. & Niiyama, Y. 2018 Two new Pseudanabaena (Cyanobacteria, Synechococcales) species from Japan, Pseudanabaena cinerea and Pseudanabaena yagii, which produce 2-methylisoborneol. Phycol. Res., 66, 291-299. Keywords: internal transcribed spacer region; Phormidiumtenue; Pseudanabaena foetida; Pseudanabaena subfoetida; rRNA operon; secondary structure Strain(s): 512, 4059, 4062, 4063, 4064, 4065, 4237, 4238 DOI: 10.1111/pre.12327 Abeynayaka, HDL., Asaeda, T., Rashid, MH. 2018 Effects of elevated pressure on Pseudanabaena galeata Böcher in varying light and dark environments. Environ. Sci. Pollut. Res., 25, 21224–21232. Keywords: Cyanobacteria; Hydrostatic pressure; Pseudanabaena galeata Böcher; Prolonged darkness; Chlorophyll-a; Cell concentration Strain(s): 512 PubMed: 29779078 DOI: 10.1007/s11356-018-2218-5 Tuji, A. & Niiyama, Y. 2018 The taxonomy of Pseudanabaena（Cyanobacteria） and characters for Jjudging the production of odor compounds. Jpn. J. Water Treat. Biol., 54, 115-120 (in Japanese with English summary). Keywords: 2－MIB; L/W ratio; Phormidium tenue; pigment; Pseudanabaena Strain(s): 512, 4059, 4062, 4063, 4064, 4065, 4237, 4238 DOI: 10.2521/jswtb.54.115 Nagai, T. 2017 Predicting herbicide mixture effects on multiple algal species using mixture toxicity models. Environ. Toxicol. Chem., 36, 2624-2630. Keywords: Pesticide; Species sensitivity distribution; Mixture toxicology; Algae; Mode of action Strain(s): 71, 487, 512, 797 PubMed: 28316094 DOI: 10.1002/etc.3800 Kaya, K., Sano, T. 2016 Chapter 18. Cyanobacterial retinoids. In Handbook of cyanobacterial monitoring and cyanotoxin analysis, Eds. byMeriluoto, J., Spoof, L., Codd, G. A., John Wiley & Sons, Ltd. pp. 173-178. Strain(s): 36, 44, 45, 46, 204, 512, 610, 1222 DOI: 10.1002/9781119068761.ch18 Tuji, A. & Niiyama, Y. 2016 The identity and phylogeny of Pseudanabaena Strain, NIES-512, producing 2-methylisoborneol (2-MIB). Bull. Natl. Mus. Nat. Sci., Ser. B, 42, 83-89. Keywords: 2-MIB; Pseudanabaena foetida var. intermedia; Pseudanabaena foetida var. subfoetida; secondary structure Strain(s): 512, 4059 Chiu, Y.-T., Yen, H.-K., Lin, T.-F. 2016 An alternativemethodtoquantify2-MIB producing cyanobacteriain in drinking water reservoirs: Method development and field applications. Environ. Res., 151, 618-627. Keywords: Cyanobacteria; 2-methylisoborneol(2-MIB); 2-MIB-synthesis gene(mibC gene); Tasteandodor; Real-timePCR Strain(s): 81, 512, 824, 905, 2104, 2116 PubMed: 27607443 DOI: 10.1016/j.envres.2016.08.034 Nagai, T., Taya, K., Yoda, I. 2016 Comparative toxicity of 20 herbicides to 5 periphytic algae and the relationship with mode of action. Environ. Toxicol. Chem., 35, 368-375. Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution Strain(s): 71, 487, 512, 797 PubMed: 26174500 DOI: 10.1002/etc.3150 Nagai, T. 2014 河川付着藻類を用いた農薬の毒性試験マニュアル Ver. 1.0 In Research Project for Environmental Risk Assessment of Nutrients and Organochemicals, NIAES, 52 pp (in Japanese). Strain(s): 71, 487, 512, 797 Kakimoto, M., Ishikawa, T., Miyagi, A., Saito, K., Miyazaki, M., Asaeda, T., Yamaguchi, M., Uchiyama, H., Kawai-Yamada, M. 2014 Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata. J. Plant Res., 171, 292-300. Keywords: 2-MIB; Cyanobacteria; Gene expression; Metabolomics; Temperature-induction Strain(s): 512 PubMed: 24140001 DOI: 10.1016/j.jplph.2013.09.005 Nagai, T., Taya, K., Annoh, H., Ishihara, S. 2013 Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species. Ecotox. Environ. Safe., 94, 37-44. Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution Strain(s): 35, 71, 73, 414, 487, 512, 797 DOI: 10.1016/j.ecoenv.2013.04.020 Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57. Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa Strain(s): 30, 40, 77, 102, 111, 230, 512, 843, 905, 928, 932, 937, 991, 1645, 1669, 1698, 1729, 1950, 2116 DOI: 10.1016/j.hal.2012.01.003 Kaya, K., Shiraishi, F., Uchida, H., Sano, T. 2011 A novel retinoic acid analogue, 7-hydroxy retinoic acid, isolated from cyanobacteria. Biochim. Biophys. Acta-Gen. Subj., 1810, 414-419. Keywords: 7-hydroxy retinoic acid; RAR agonistic activity; cyanobacteria; UV stability Strain(s): 44, 45, 46, 512, 610, 931 PubMed: 21145941 DOI: 10.1016/j.bbagen.2010.11.009 Iwase, S., Abe, T. 2010 Identification and change in concentration of musty-odor compounds during frowth in blue-green algae. J. Sch. Marine Sci. Tech., Tokai Univ., 8, 27-33. Strain(s): 512, 2119 Ohko, Y., Nagao, Y., Okano, K., Sugiura, N., Fukuda, A., Yang, Y., Negishi, N., Takeuchi, M., Hanada, S. 2009 Prevention of Phormidium tenue biofilm formation by TiO₂ photocatalysis. Microbes Environ., 24, 241-245. Keywords: Phormidium tenue; cyanobacteria; TiO₂ photocatalyst; anti-biofilm; UV light Strain(s): 512 DOI: 10.1264/jsme2.ME09106 Ishibashi, Y., Oikawa, E., Honda, H., Adachi, T., Nakatochi, M. 2007 Analyses of growth factors of taste and odor producing algae by a fuzzy neural network. Environ. Eng. Res. [Kankyô-kôgaku Kenkyû Ronbunshû], 44, 255-263 (in Japanese with English summary). Keywords: musty odor; Phormidium tenue; Uroglena americana; fuzzy neural network; wind speed Strain(s): 512 DOI: 10.11532/proes1992.44.255 Oshimi, M., Fujimoto, N., Ohnishi, A., Suzuki, M., Akiba, M., Kunikane, S. 2005 Isolation of bacteria lysing musty odor producing alga Phormidium tenue and its lytic characteristics. J. Jpn. Soc. Wat. Environ., 28, 379-384 (in Japanese with English summary). Keywords: alga-lytic bacteria; musty odor; Phormidium tenue; Pseudomonas KA6 Strain(s): 96, 211, 227, 244, 298, 512, 817 DOI: 10.2965/jswe.28.379 Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8. Keywords: cyanobacteria; homospermidine; polyamine; spermidine Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277 Oikawa, E. Ishibashi, Y., 2004 Species speciﬁcity of musty odor producing Phormidium tenue in Lake Kamafusa. Wat. Sci. Tech., 49, 41-46. Keywords: 16S-rDNA sequence; 2-methylisoborneol; musty odor; Phormidium tenue phylogenetic tree Strain(s): 21, 30, 33, 41, 73, 76, 512, 611 PubMed: 15237605 DOI: 10.2166/wst.2004.0529 Zhou, S., Nakai, S., Hosomi, M., Sezaki, Y., Tominaga, M. 2004 Inhibition of cyanobacterial growth by allelopathy of reed. Jpn. J. Water Treat. Biol., 40, 23-28 (in Japanese with English summary). Keywords: reed; Phormidium tenue; cyanobacteria; inhibition; allelopathy Strain(s): 512 DOI: 10.2521/jswtb.40.23 Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Oikawa, E., Ishibashi, Y., Abe, T., Umetsu, H. 2000 Phylogenetic classification of musty odor and/or toxic compounds producing cyanobacteria. Environ. Eng. Res., 37, 183-191 (in Japanese with English summary). Keywords: cyanobacteria; musty odor; 16S ribosomal RNA; PCR; phylogenetic trees Strain(s): 21, 30, 33, 41, 73, 76, 512 DOI: 10.11532/proes1992.37.183 Nakai, S., Yamane, S., Hosomi, M. 2000 Algal growth inhibition effects of Myriophyllum spicatum-releasing four allelopathic polyphenols. J. Jpn. Soc. Wat. Environ., 23, 726-730 (in Japanese with English summary). Keywords: allelochemical; polyphenol; algae; concentration-dependent synergistic growth inhibition; autoxidation Strain(s): 35, 71, 87, 96, 512 Nakai, S., Inoue, Y., Hosomi, M., Murakami, A. 1999 Growth inhibition of blue-green algae by allelopathic effects of macrophytes. Wat. Sci. Tech., 39, 47-53. Keywords: allelopathy; blue-green algae; coexistence; growth inhibition; macrophyte; Myriophyllum spicatum Strain(s): 75, 87, 512 DOI: 10.1016/S0273-1223(99)00185-7 Lee, T., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. 1995 Quantitative determination of cyanobacteria in mixed phytoplankton assemblages by an in vivo fluorimetric method. Anal. Chim. Acta, 302, 81-87. Keywords: cyanobacteria; quantitative estimation; phycocyanin; waterblooms; Microcystis aeruginosa Strain(s): 298, 512 DOI: 10.1016/0003-2670(94)00425-L Lee, T., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. 1994 In vivo fluorometric method for early detection of cyanobacterial waterblooms. J. Appl. Phycol., 6, 489-495. Keywords: cyanobacteria; waterblooms; in vivo fluorescence; monitoring; Microcystis aeruginosa Strain(s): 298, 512 Oikawa, E., Ohnuma, T., Shimizu, A., Ishibashi, Y. 1994 Phylogenetic studies on musty odor producing cyanobacteria using the 16S ribosomal RNA [16S ribosômu RNA enki-hairetsu no hikaku ni yoru kabi-shû sansei ransô-rui no bunrui]. J. Jpn. Water Works Assoc. [Suidô-Kyôkai Zasshi], 63(12), 56-63 (in Japanese). Keywords: musty odor; gene; cyanobacteria Strain(s): 36, 76, 512 Yamada, N., Aoyama, K., Yamada, M., Hamamura, N. 1986 Studies on earthy-musty odor in natural water (3). Isolation of bacteria-free Phormidium tenue and the effect of associated bacteria on the growth of axenic P. tenue. Jpn. J. Water Poll. Res., 9, 379-385 (in Japanese with English summary). Keywords: Phormidium tenue; axenic; growth; 2-methylisoborneol; bacteria interaction Strain(s): 512 Yamada, N., Aoyama, K., Yamada, M., Hamamura, N. 1985 Studies on earthy-musty odor in natural water (1). Growth characteristics and 2-methylisoborneol production of Phormidium tenue. Jpn. J. Water Poll. Res., 8, 515-521 (in Japanese with English summary). Keywords: blue-green algae; Phormidium tenue; growth characteristics; headspace-GC analysis; 2-methylisoborneol Strain(s): 512

Reference

Dayarathne, K., Ishikawa, T., Kadeer, A., Yamaguchi, M., Kawai-Yamada, M. 2024 The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia. , Arch. Microbiol., 367 (article ID).
Keywords: Cyanobacteria; 2-MIB; Gene expression; Light-dependent
Strain(s): 512
DOI: 10.1007/s00203-024-04099-w

Senavirathna, M. D. H. J. & Jayasekara, M. A. D. D. 2023 Temporal variation of 2-MIB and geosmin production by Pseudanabaena galeata and Phormidium ambiguum exposed to high-intensity light. Water Environ. Res., 95, e10834 (article ID).
Keywords: extreme light; filamentous cyanobacteria; growth suppression; odorous compounds; stress response
Strain(s): 512, 2119
PubMed: 36635233
DOI: 10.1002/wer.10834

Christodoulou, M., Wahlsten, M., Sivonen, K. 2023 Morphological and Molecular Evaluation of Pseudanabaena epilithica sp. nov. and P. suomiensis sp. nov. (Pseudanabaenaceae, Cyanobacteria) from Finland. Diversity-Basel, 15, 909 (article ID).
Keywords: Pseudanabaena; cyanobacteria; polyphasic approach; 16S rRNA gene; 16S–23S ITS; Finland
Strain(s): 512, 4062, 4063, 4064, 4065, 4237, 4238
DOI: 10.3390/d15080909

Lee, J. E., Yu, M. N., Yu, S., Byeon, M. 2022 Occurrence and phylogenetic analysis of Pseudanabaena sp. producing 2-methylisoborneol in drinking water source of South Korea. Environ. Microbiol. Rep., 14, 197-202.
Strain(s): 512
PubMed: 34837342
DOI: 10.1111/1758-2229.13031

Suruzzaman, M., Cao, T., Lu, J., Wang, Y., Su, M., Yang, M. 2022 Evaluation of the MIB-producing potential based on real-time qPCR in drinking water reservoirs. Environ. Res., 204, 112308 (article ID).
Keywords: 2-Methylisoborneol (MIB); Taste & odor; Real time qPCR; MIB-Synthesis (mic) gene; Drinking water
Strain(s): 512, 4062, 4063, 4237, 4238
PubMed: 34757030
DOI: 10.1016/j.envres.2021.112308

Shen, O., Wang, Q., Miao, H., Shimada, M., Utsumi, M., Lei, Z., Zhang, Z., Nishimura, O., Asada, Y., Fujimoto, N., Takanashi, H., Akiba, M., Shimizu, K. 2021 Temperature affects growth, geosmin/2-methylisoborneol production, and gene expression in two cyanobacterial species. Environ. Sci. Pollut. Res., 29, 12017–12026.
Keywords: Cyanobacteria; Temperature; Cell growth; Geosmin; 2-MIB; Gene expression
Strain(s): 512, 824
PubMed: 34558048
DOI: 10.1007/s11356-021-16593-5

Devi, A., Chiu, Y.-T., Hsueh, H.-T., Lin, T.-F. 2021 Quantitative PCR based detection system for cyanobacterial geosmin/2-methylisoborneol (2-MIB) events in drinking water sources: Current status and challenges. Water Res., 188, 116478 (article ID).
Keywords: biomolecular; drinking water; database; geo; mic; monitoring; Taq-Man probes
Strain(s): 512, 4062, 4063, 4237, 4238
PubMed: 33045635
DOI: 10.1016/j.watres.2020.116478

Kim, K., Yoon, Y., Cho, H., Hwang, S. J 2020 Molecular probes to evaluate the synthesis and production potential of an odorous compound (2-methylisoborneol) in cyanobacteria. Int. J. Environ. Res. Public Health, 17, 1933 (article ID).
Keywords: 2-Methylisoborneol (2-MIB); cyanobacteria; molecular probes; mibC; real-time PCR
Strain(s): 19, 512, 824
PubMed: 32188031
DOI: 10.3390/ijerph17061933

Muhetaer, G., Asaeda, T., Jayasanka, S. M. D. H., Baniya, M. B., Abeynayaka, H. D. L., Rashid, M. H., Yan, H. Y. 2020 Effects of light intensity and exposure period on the growth and stress responses of two cyanobacteria species: Pseudanabaena galeata and Microcystis aeruginosa. Water, 12, 407 (article ID).
Keywords: cyanobacterial growth; stress responses; Pseudanabaena galeata; Microcystis aeruginosa; oxidative stress; antioxidative enzymes
Strain(s): 111, 512
PubMed: 32045866
DOI: 10.3390/w12020407

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Oliveira, E. D. C. de, Cunha, A. C. da, Silva, N. B. da, Castelo-Branco, R., Morais, J., Schneider, M. P. C., Faustino, S. M. M., Ramos, V., Vasconcelos, V. 2019 Morphological and molecular characterization of cyanobacterial isolates from the mouth of the Amazon River. Phytotaxa, 387, 269-288.
Keywords: Amazonia; Limnothrix; Polyphasic approach; Pseudanabaena; 16S rRNA phylogeny; Algae
Strain(s): 504, 512
DOI: 10.11646/phytotaxa.387.4.1

Strain(s): 75, 333, 512, 805, 1642, 1666, 1677, 1696, 1723
DOI: 10.34566/jwwa.88.12_3

Tuji, A. & Niiyama, Y. 2018 Two new Pseudanabaena (Cyanobacteria, Synechococcales) species from Japan, Pseudanabaena cinerea and Pseudanabaena yagii, which produce 2-methylisoborneol. Phycol. Res., 66, 291-299.
Keywords: internal transcribed spacer region; Phormidiumtenue; Pseudanabaena foetida; Pseudanabaena subfoetida; rRNA operon; secondary structure
Strain(s): 512, 4059, 4062, 4063, 4064, 4065, 4237, 4238
DOI: 10.1111/pre.12327

Abeynayaka, HDL., Asaeda, T., Rashid, MH. 2018 Effects of elevated pressure on Pseudanabaena galeata Böcher in varying light and dark environments. Environ. Sci. Pollut. Res., 25, 21224–21232.
Keywords: Cyanobacteria; Hydrostatic pressure; Pseudanabaena galeata Böcher; Prolonged darkness; Chlorophyll-a; Cell concentration
Strain(s): 512
PubMed: 29779078
DOI: 10.1007/s11356-018-2218-5

Tuji, A. & Niiyama, Y. 2018 The taxonomy of Pseudanabaena（Cyanobacteria） and characters for Jjudging the production of odor compounds. Jpn. J. Water Treat. Biol., 54, 115-120 (in Japanese with English summary).
Keywords: 2－MIB; L/W ratio; Phormidium tenue; pigment; Pseudanabaena
Strain(s): 512, 4059, 4062, 4063, 4064, 4065, 4237, 4238
DOI: 10.2521/jswtb.54.115

Nagai, T. 2017 Predicting herbicide mixture effects on multiple algal species using mixture toxicity models. Environ. Toxicol. Chem., 36, 2624-2630.
Keywords: Pesticide; Species sensitivity distribution; Mixture toxicology; Algae; Mode of action
Strain(s): 71, 487, 512, 797
PubMed: 28316094
DOI: 10.1002/etc.3800

Kaya, K., Sano, T. 2016 Chapter 18. Cyanobacterial retinoids. In Handbook of cyanobacterial monitoring and cyanotoxin analysis, Eds. byMeriluoto, J., Spoof, L., Codd, G. A., John Wiley & Sons, Ltd. pp. 173-178.
Strain(s): 36, 44, 45, 46, 204, 512, 610, 1222
DOI: 10.1002/9781119068761.ch18

Tuji, A. & Niiyama, Y. 2016 The identity and phylogeny of Pseudanabaena Strain, NIES-512, producing 2-methylisoborneol (2-MIB). Bull. Natl. Mus. Nat. Sci., Ser. B, 42, 83-89.
Keywords: 2-MIB; Pseudanabaena foetida var. intermedia; Pseudanabaena foetida var. subfoetida; secondary structure
Strain(s): 512, 4059

Chiu, Y.-T., Yen, H.-K., Lin, T.-F. 2016 An alternativemethodtoquantify2-MIB producing cyanobacteriain in drinking water reservoirs: Method development and field applications. Environ. Res., 151, 618-627.
Keywords: Cyanobacteria; 2-methylisoborneol(2-MIB); 2-MIB-synthesis gene(mibC gene); Tasteandodor; Real-timePCR
Strain(s): 81, 512, 824, 905, 2104, 2116
PubMed: 27607443
DOI: 10.1016/j.envres.2016.08.034

Nagai, T., Taya, K., Yoda, I. 2016 Comparative toxicity of 20 herbicides to 5 periphytic algae and the relationship with mode of action. Environ. Toxicol. Chem., 35, 368-375.
Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution
Strain(s): 71, 487, 512, 797
PubMed: 26174500
DOI: 10.1002/etc.3150

Nagai, T. 2014 河川付着藻類を用いた農薬の毒性試験マニュアル Ver. 1.0 In Research Project for Environmental Risk Assessment of Nutrients and Organochemicals, NIAES, 52 pp (in Japanese).
Strain(s): 71, 487, 512, 797

Kakimoto, M., Ishikawa, T., Miyagi, A., Saito, K., Miyazaki, M., Asaeda, T., Yamaguchi, M., Uchiyama, H., Kawai-Yamada, M. 2014 Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata. J. Plant Res., 171, 292-300.
Keywords: 2-MIB; Cyanobacteria; Gene expression; Metabolomics; Temperature-induction
Strain(s): 512
PubMed: 24140001
DOI: 10.1016/j.jplph.2013.09.005

Nagai, T., Taya, K., Annoh, H., Ishihara, S. 2013 Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species. Ecotox. Environ. Safe., 94, 37-44.
Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution
Strain(s): 35, 71, 73, 414, 487, 512, 797
DOI: 10.1016/j.ecoenv.2013.04.020

Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identiﬁcation of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 30, 40, 77, 102, 111, 230, 512, 843, 905, 928, 932, 937, 991, 1645, 1669, 1698, 1729, 1950, 2116
DOI: 10.1016/j.hal.2012.01.003

Kaya, K., Shiraishi, F., Uchida, H., Sano, T. 2011 A novel retinoic acid analogue, 7-hydroxy retinoic acid, isolated from cyanobacteria. Biochim. Biophys. Acta-Gen. Subj., 1810, 414-419.
Keywords: 7-hydroxy retinoic acid; RAR agonistic activity; cyanobacteria; UV stability
Strain(s): 44, 45, 46, 512, 610, 931
PubMed: 21145941
DOI: 10.1016/j.bbagen.2010.11.009

Iwase, S., Abe, T. 2010 Identification and change in concentration of musty-odor compounds during frowth in blue-green algae. J. Sch. Marine Sci. Tech., Tokai Univ., 8, 27-33.
Strain(s): 512, 2119

Ohko, Y., Nagao, Y., Okano, K., Sugiura, N., Fukuda, A., Yang, Y., Negishi, N., Takeuchi, M., Hanada, S. 2009 Prevention of Phormidium tenue biofilm formation by TiO₂ photocatalysis. Microbes Environ., 24, 241-245.
Keywords: Phormidium tenue; cyanobacteria; TiO₂ photocatalyst; anti-biofilm; UV light
Strain(s): 512
DOI: 10.1264/jsme2.ME09106

Ishibashi, Y., Oikawa, E., Honda, H., Adachi, T., Nakatochi, M. 2007 Analyses of growth factors of taste and odor producing algae by a fuzzy neural network. Environ. Eng. Res. [Kankyô-kôgaku Kenkyû Ronbunshû], 44, 255-263 (in Japanese with English summary).
Keywords: musty odor; Phormidium tenue; Uroglena americana; fuzzy neural network; wind speed
Strain(s): 512
DOI: 10.11532/proes1992.44.255

Oshimi, M., Fujimoto, N., Ohnishi, A., Suzuki, M., Akiba, M., Kunikane, S. 2005 Isolation of bacteria lysing musty odor producing alga Phormidium tenue and its lytic characteristics. J. Jpn. Soc. Wat. Environ., 28, 379-384 (in Japanese with English summary).
Keywords: alga-lytic bacteria; musty odor; Phormidium tenue; Pseudomonas KA6
Strain(s): 96, 211, 227, 244, 298, 512, 817
DOI: 10.2965/jswe.28.379

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 19, 21, 22, 28, 30, 34, 37, 41, 44, 45, 50, 73, 78, 79, 80, 81, 204, 208, 510, 512, 515, 592, 593, 610, 843, 846, 847, 2093, 2096, 2098, 2100, 2102, 2107, 2108, 2118, 2119, 2123, 2124, 2125, 2126, 2128, 2129, 2131, 2132, 3259, 3275, 3276, 3277

Oikawa, E. Ishibashi, Y., 2004 Species speciﬁcity of musty odor producing Phormidium tenue in Lake Kamafusa. Wat. Sci. Tech., 49, 41-46.
Keywords: 16S-rDNA sequence; 2-methylisoborneol; musty odor; Phormidium tenue phylogenetic tree
Strain(s): 21, 30, 33, 41, 73, 76, 512, 611
PubMed: 15237605
DOI: 10.2166/wst.2004.0529

Zhou, S., Nakai, S., Hosomi, M., Sezaki, Y., Tominaga, M. 2004 Inhibition of cyanobacterial growth by allelopathy of reed. Jpn. J. Water Treat. Biol., 40, 23-28 (in Japanese with English summary).
Keywords: reed; Phormidium tenue; cyanobacteria; inhibition; allelopathy
Strain(s): 512
DOI: 10.2521/jswtb.40.23

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Oikawa, E., Ishibashi, Y., Abe, T., Umetsu, H. 2000 Phylogenetic classification of musty odor and/or toxic compounds producing cyanobacteria. Environ. Eng. Res., 37, 183-191 (in Japanese with English summary).
Keywords: cyanobacteria; musty odor; 16S ribosomal RNA; PCR; phylogenetic trees
Strain(s): 21, 30, 33, 41, 73, 76, 512
DOI: 10.11532/proes1992.37.183

Nakai, S., Yamane, S., Hosomi, M. 2000 Algal growth inhibition effects of Myriophyllum spicatum-releasing four allelopathic polyphenols. J. Jpn. Soc. Wat. Environ., 23, 726-730 (in Japanese with English summary).
Keywords: allelochemical; polyphenol; algae; concentration-dependent synergistic growth inhibition; autoxidation
Strain(s): 35, 71, 87, 96, 512

Nakai, S., Inoue, Y., Hosomi, M., Murakami, A. 1999 Growth inhibition of blue-green algae by allelopathic effects of macrophytes. Wat. Sci. Tech., 39, 47-53.
Keywords: allelopathy; blue-green algae; coexistence; growth inhibition; macrophyte; Myriophyllum spicatum
Strain(s): 75, 87, 512
DOI: 10.1016/S0273-1223(99)00185-7

Lee, T., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. 1995 Quantitative determination of cyanobacteria in mixed phytoplankton assemblages by an in vivo fluorimetric method. Anal. Chim. Acta, 302, 81-87.
Keywords: cyanobacteria; quantitative estimation; phycocyanin; waterblooms; Microcystis aeruginosa
Strain(s): 298, 512
DOI: 10.1016/0003-2670(94)00425-L

Lee, T., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. 1994 In vivo fluorometric method for early detection of cyanobacterial waterblooms. J. Appl. Phycol., 6, 489-495.
Keywords: cyanobacteria; waterblooms; in vivo fluorescence; monitoring; Microcystis aeruginosa
Strain(s): 298, 512

Oikawa, E., Ohnuma, T., Shimizu, A., Ishibashi, Y. 1994 Phylogenetic studies on musty odor producing cyanobacteria using the 16S ribosomal RNA [16S ribosômu RNA enki-hairetsu no hikaku ni yoru kabi-shû sansei ransô-rui no bunrui]. J. Jpn. Water Works Assoc. [Suidô-Kyôkai Zasshi], 63(12), 56-63 (in Japanese).
Keywords: musty odor; gene; cyanobacteria
Strain(s): 36, 76, 512

Yamada, N., Aoyama, K., Yamada, M., Hamamura, N. 1986 Studies on earthy-musty odor in natural water (3). Isolation of bacteria-free Phormidium tenue and the effect of associated bacteria on the growth of axenic P. tenue. Jpn. J. Water Poll. Res., 9, 379-385 (in Japanese with English summary).
Keywords: Phormidium tenue; axenic; growth; 2-methylisoborneol; bacteria interaction
Strain(s): 512

Yamada, N., Aoyama, K., Yamada, M., Hamamura, N. 1985 Studies on earthy-musty odor in natural water (1). Growth characteristics and 2-methylisoborneol production of Phormidium tenue. Jpn. J. Water Poll. Res., 8, 515-521 (in Japanese with English summary).
Keywords: blue-green algae; Phormidium tenue; growth characteristics; headspace-GC analysis; 2-methylisoborneol
Strain(s): 512

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