Strain Data

Strain number		NIES-2212
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Chlamydomonas debaryana Goroschankin
Synonym
Former name		Chlamydomonas angulosa Dill
Common name		Green alga
Locality (Date of collection)		Kobe, Hyogo Japan
Latitude / Longitude		34.689404 / 135.192204
Habitat (Isolation source)
History		< IAM (2007) < UTEX (1996);
Isolator (Date of isolation)		Tsubo, Yoshihiro
Identified by
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 C Light intensity: 3-5 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 6 M
Gene information		Whole-genome ( BDDB00000000 ) , Mitochondrial DNA ( MT872349 ) , 18S rRNA ( AB701542 ) , PLMT ( LC228968 )
Cell size (min - max)		7 - 12 μm
Organization		Unicellular; Flagellate
Characteristics		Phototaxis ; Starch production (Toyoshima et al. 2015) ; Genome decoded strain (Hirashima et al. 2016)
Other strain no.		Other collection strain no. : IAM C-549 (=C-1); Tsubo I16; UTEX 618; CCAP 11/59; SAG 4.72
Remarks		Cryopreserved; Axenic
Movie

Reference
Smith, D. R. & Craig, R. J. 2021 Does mitochondrial DNA replication in Chlamydomonas require a reverse transcriptase? New Phytol., 229, 1192-1195. Strain(s): 2212 PubMed: 32936939 DOI: 10.1111/nph.16930 Matsuzaki, R., Suzuki, S., Yamaguchi, H., Kawachi, M., Kanesaki, Y., Yoshikawa, H., Mori, T., Nozaki, H. 2021 The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid. BMC Ecol. Evol., 21, 11 (article ID). Keywords: Chloromonas; Evolution; Green algae; Hydrophobicity of RBCS helices; Pyrenoid; Pyrenoid loss; Rubisco small subunit (RBCS) Strain(s): 144, 424, 425, 2207, 2212, 2215, 2242, 2243, 2499, 3753, 4284 PubMed: 33514317 DOI: 10.1186/s12862-020-01733-1 Yoshitomi, T., Kaminaga, S., Sato, N., Toyoshima, M., Moriyama, T., Yoshimoto, K. 2020 Formation of spherical palmelloid colony with enhanced lipid accumulation by gel encapsulation of Chlamydomonas debaryana NIES-2212. Plant Cell Physiol., 61, 158–168. Keywords: Chlamydomonas; Acyltransferase; Alginate gel; Gel encapsulation; Palmelloid colony; Triacylglycerol Strain(s): 2212 PubMed: 31589321 DOI: 10.1093/pcp/pcz188 Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133. Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS Strain(s): 35, 144, 362, 418, 421, 432, 438, 456, 545, 571, 732, 737, 858, 1022, 1048, 1848, 2150, 2169, 2201, 2202, 2208, 2209, 2212, 2280, 2352, 2462, 2578 PubMed: 30304548 DOI: 10.1111/jpy.12798 Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76. Keywords: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase Strain(s): 2202, 2207, 2212, 2242 PubMed: 29330556 DOI: 10.1007/s00239-017-9826-4 Toyoshima, M. & Sato, N. 2018 Optimization of triacylglycerol and starch production in Chlamydomonas debaryana NIES-2212 with regard to light intensity and CO₂ concentration. Microbiology, 164, 359-368. Keywords: light intensity; CO2 supplement; TAG; Chlamydomonas debaryana; starch Strain(s): 2212 PubMed: 29458672 DOI: 10.1099/mic.0.000603 Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146. Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase Strain(s): 2202, 2207, 2212, 2235, 2242 PubMed: 2848352 DOI: 10.1016/j.bbrc.2017.05.026 Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID). Strain(s): 2202, 2207, 2212, 2242 PubMed: 27688324 DOI: 10.1128/genomeA.01070-16 Takahashi, K., Moestrup, Ø., Jordan, R. W., Iwataki, M. 2015 High-level accumulation of triacylglycerol and starch in photoautotrophically grown Chlamydomonas debaryana NIES-2212. Plant Cell Physiol., 56, 2447-2456. Keywords: Biofuel; Chlamydomonas debaryana NIES-2212; Fatty acid; Starch; Triacylglycerol Strain(s): 2212 PubMed: 26542110 DOI: 10.1093/pcp/pcv163 Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650. Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine Strain(s): 2202, 2207, 2212, 2215, 2219, 2225, 2230, 2242, 2316, 2462, 2499 PubMed: 24947506 DOI: 10.1007/s10265-014-0644-0 Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12. Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284 Nozaki, H., Mori, T., Misumi, O., Matsunaga, S., Kuroiwa, T. 2006 Males evolved from the dominant isogametic mating type. Curr. Biol., 16, R1018-R1020. Strain(s): 1611, 1614, 2212, 2213, 2214 PubMed: 17174904 DOI: 10.1016/j.cub.2006.11.019 Sakaguchi, T., Tsuji, T., Nakajima, A., Horikoshi, T. 1979 Accumulation of cadmium by green microalgae. Eur. J. Appl. Microbiol. Biotechnol., 8, 207-215. Strain(s): 2212, 2273, 2274, 2279 DOI: 10.1007/BF00506184 Nakajima, A., Horikoshi, T., Sakaguchi, T. 1979 Uptake of copper ion by green microalgae. Agric. Biol. Chem., 43, 1455-1460. Strain(s): 2212, 2273, 2274, 2279 DOI: 10.1080/00021369.1979.10863659 Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565. Strain(s): 2138, 2139, 2212, 2238, 2279

Reference

Smith, D. R. & Craig, R. J. 2021 Does mitochondrial DNA replication in Chlamydomonas require a reverse transcriptase? New Phytol., 229, 1192-1195.
Strain(s): 2212
PubMed: 32936939
DOI: 10.1111/nph.16930

Matsuzaki, R., Suzuki, S., Yamaguchi, H., Kawachi, M., Kanesaki, Y., Yoshikawa, H., Mori, T., Nozaki, H. 2021 The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid. BMC Ecol. Evol., 21, 11 (article ID).
Keywords: Chloromonas; Evolution; Green algae; Hydrophobicity of RBCS helices; Pyrenoid; Pyrenoid loss; Rubisco small subunit (RBCS)
Strain(s): 144, 424, 425, 2207, 2212, 2215, 2242, 2243, 2499, 3753, 4284
PubMed: 33514317
DOI: 10.1186/s12862-020-01733-1

Yoshitomi, T., Kaminaga, S., Sato, N., Toyoshima, M., Moriyama, T., Yoshimoto, K. 2020 Formation of spherical palmelloid colony with enhanced lipid accumulation by gel encapsulation of Chlamydomonas debaryana NIES-2212. Plant Cell Physiol., 61, 158–168.
Keywords: Chlamydomonas; Acyltransferase; Alginate gel; Gel encapsulation; Palmelloid colony; Triacylglycerol
Strain(s): 2212
PubMed: 31589321
DOI: 10.1093/pcp/pcz188

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133.
Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS
Strain(s): 35, 144, 362, 418, 421, 432, 438, 456, 545, 571, 732, 737, 858, 1022, 1048, 1848, 2150, 2169, 2201, 2202, 2208, 2209, 2212, 2280, 2352, 2462, 2578
PubMed: 30304548
DOI: 10.1111/jpy.12798

Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76.
Keywords: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase
Strain(s): 2202, 2207, 2212, 2242
PubMed: 29330556
DOI: 10.1007/s00239-017-9826-4

Toyoshima, M. & Sato, N. 2018 Optimization of triacylglycerol and starch production in Chlamydomonas debaryana NIES-2212 with regard to light intensity and CO₂ concentration. Microbiology, 164, 359-368.
Keywords: light intensity; CO2 supplement; TAG; Chlamydomonas debaryana; starch
Strain(s): 2212
PubMed: 29458672
DOI: 10.1099/mic.0.000603

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 2202, 2207, 2212, 2235, 2242
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017.05.026

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2202, 2207, 2212, 2242
PubMed: 27688324
DOI: 10.1128/genomeA.01070-16

Takahashi, K., Moestrup, Ø., Jordan, R. W., Iwataki, M. 2015 High-level accumulation of triacylglycerol and starch in photoautotrophically grown Chlamydomonas debaryana NIES-2212. Plant Cell Physiol., 56, 2447-2456.
Keywords: Biofuel; Chlamydomonas debaryana NIES-2212; Fatty acid; Starch; Triacylglycerol
Strain(s): 2212
PubMed: 26542110
DOI: 10.1093/pcp/pcv163

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 2202, 2207, 2212, 2215, 2219, 2225, 2230, 2242, 2316, 2462, 2499
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284

Nozaki, H., Mori, T., Misumi, O., Matsunaga, S., Kuroiwa, T. 2006 Males evolved from the dominant isogametic mating type. Curr. Biol., 16, R1018-R1020.
Strain(s): 1611, 1614, 2212, 2213, 2214
PubMed: 17174904
DOI: 10.1016/j.cub.2006.11.019

Sakaguchi, T., Tsuji, T., Nakajima, A., Horikoshi, T. 1979 Accumulation of cadmium by green microalgae. Eur. J. Appl. Microbiol. Biotechnol., 8, 207-215.
Strain(s): 2212, 2273, 2274, 2279
DOI: 10.1007/BF00506184

Nakajima, A., Horikoshi, T., Sakaguchi, T. 1979 Uptake of copper ion by green microalgae. Agric. Biol. Chem., 43, 1455-1460.
Strain(s): 2212, 2273, 2274, 2279
DOI: 10.1080/00021369.1979.10863659

Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565.
Strain(s): 2138, 2139, 2212, 2238, 2279

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