Strain Data

Strain number		NIES-2173
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Chlorella sorokiniana Shihira & R.W.Krauss
Synonym
Former name		Chlorella vulgaris Beijerinck
Common name		Green alga
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < Tsuzuki, Mikio (1988) < Avramova, S. T. (1982) < probably Semenenko
Isolator (Date of isolation)
Identified by		Hoshina, Ryo (Reidentify)
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2020 Sept]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M
Gene information		18S rRNA ( AB080308 ) , actin-1 ( AB080312 ) , actin-2 ( AB080313 ) , 18S-ITS1-5.8S-ITS2-28S ( AB731601 AB731602 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Starch production (Miyachi et al. 1986)
Other strain no.		Other collection strain no. : IAM C-536; IPPAS C-3
Remarks		Cryopreserved; Axenic
Movie

Reference
Krivina, E., Portnov, A., Temraleeva, A. 2024 A description of Aliichlorella ignota gen. et sp. nov. and a comparison of the efficiency of species delimitation methods in the Chlorella-clade (Trebouxiophyceae, Chlorophyta). Phycol. Res., 72, 180-190. Strain(s): 455, 2167, 2171, 2173, 2330, 4032, 4033, 4034 DOI: 10.1111/pre.12551 Ramírez‑Romero, A., Da Costa Magalhães, B., Matricon, L., Sassi, J.-F., Steyer, J.-P., Delrue, F. 2024 Aqueous phase recycling: impact on microalgal lipid accumulation and biomass quality. Environ. Sci. Pollut. Res., , Keywords: Renewable feedstock; Hydrothermal liquefaction; Green fuels; Microalgal biomass; Nutrient cycling Strain(s): 227, 2173 PubMed: 38438644 DOI: 10.1007/s11356-024-32701-7 Nishi, K. 2023 Development of advanced light-tolerant microalgae-nitrifying bacteria consortia for ammonia removal under strong light irradiation using light-shielding hydrogel. PhD thesis, Soka University, Japan, 94 pp. Strain(s): 2173 Marinič, D., Grilc, M., Hočevar, B., Delrue, F., Likozar, B. 2023 Liquefaction, cracking and hydrogenation of microalgae biomass resources to CO2 negative advanced biofuels: Mechanisms, reaction microkinetics and modelling. Renew. Energy, 203, 382-393. Keywords: Microalgae; Biofuel; Liquefaction; Hydrodeoxygenation; One-pot reaction Strain(s): 2173 DOI: 10.1016/j.renene.2022.12.055 Natori, N., Kuwata, M., Tanaka, K., Toda, T. 2023 Plankton survey method based on scanning electron microscopy. Bull. Plankton Soc. Japan, 70, 1-9 (in Japanese with English summary). Keywords: whole effluent toxicity; drainage management; Raphidocelis subcapitata; johkasou Strain(s): 2173 DOI: 10.24763/bpsj.70.1_1 Ramírez-Romero, A., Da Costa Magalhães, B., Dimitriades-Lemaire, A., Sassi, J.-F., Delrue, F., Steyer, J.-P. 2022 Chlorellaceae feedstock selection under balanced nutrient limitation. FERMENTATION, 8, 554 (article ID). Keywords: biofuel; microalgal biomass; Chlorella; lipids Strain(s): 227, 2173 DOI: 10.3390/fermentation8100554 Nishi, K., Akizuki, S., Toda, T., Matsuyama, T., Ida, J. 2022 Advanced light-tolerant microalgae-nitrifying bacteria consortia for stable ammonia removal under strong light irradiation using light-shielding hydrogel. Chemosphere, 297, 134252 (article ID). Keywords: Microalgae–nitrifying bacteria consortia; Photoinhibition; Light-shielding; Free ammonia; Immobilisation Strain(s): 2173 PubMed: 35271892 DOI: 10.1016/j.chemosphere.2022.134252 Qian, J., Shimotori, K., Liu, X., Ban, S., Akizuki, S., Fujiwara, M., Kodera, T., Toda, T., Imai, A. 2021 Enhancement of algal growth by Mg2+ released from anaerobic digestion effluent of aquatic macrophytes through photolysis. Biochem. Eng. J., 172, 108065 (article ID). Keywords: Ultraviolet photolysis; DOM degradation; DOM molecular size distribution; Mg availability; Bio-digester effluent Strain(s): 2173 DOI: 10.1016/j.bej.2021.108065 Sekine, M. 2021 Coupling process of simultaneous desulfurization–nitrification and microalgal cultivation for biogas and effluent from anaerobic digestion. PhD thesis, Soka University, Japan, 128 pp. Strain(s): 2173 Kishi, M., Onouchi, H., Tanaka, K., Yoshida, A., Toda, T. 2021 Simultaneous microalgal culture and biogas upgrading using anaerobic digestion effluent. Bull. Plankton Eco-Eng. Res., 1, 58-73 (in Japanese with English summary). Strain(s): 2173 Akizuki, S., Cuevas-Rodríguez, G., Toda, T. 2021 Nitrification of anaerobic digestate using a consortium of microalgae and nitrifiers in an open photobioreactor with moving bed carriers. Chemosphere, 263, 127948 (article ID). Keywords: Bacterial immobilization; light attenuation; light stress; high-ammonia wastewater; photo-oxygenation Strain(s): 2173 PubMed: 33297018 DOI: 10.1016/j.chemosphere.2020.127948 Akizuki, S., Kishi, M., Cuevas-Rodríguez, G., Toda, T. 2020 Effects of different light conditions on ammonium removal in a consortium of microalgae and partial nitrifying granules. Water Res., 171, 115445 (article ID). Keywords: Energy-saving process; Photo-oxygenation; Short-cut nitrogen removal; Ammonium removal pathway; Empirical model Strain(s): 2173 PubMed: 31954203 DOI: 10.1016/j.watres.2019.115445 Krivina, E. S. & Temraleeva, A. D. 2020 Identification problems and cryptic diversity of Chlorella-clade microalgae (Chlorophyta). Microbiology, 89, 720-732. Keywords: microalgae; morphology; ecology; phylogeny; 18S−ITS1−5.8S−ITS2 fragments; polyphasic approach Strain(s): 2167, 2173, 4032, 4034 DOI: 10.1134/S0026261720060107 Qian, J., Liu, X, Ban, S, Fujiwara, M, Kodera, T, Akizuki, S, Toda, T. 2020 pH treatments in continuous cultivation to maximize microalgal production and nutrient removal from anaerobic digestion effluent of aquatic macrophytes. J. Appl. Phycol., 32, 3349–3362. Keywords: Biomass recovery; Flow-through cultivation; Nutrient removal; pH control; Mg availability; Bio-digester effluent Strain(s): 2173 DOI: 10.1007/s10811-020-02196-z Akizuki, S., Cuevas-Rodríguez, G., Toda, T. 2020 Anaerobic digestion effluent treatment using microalgae and nitrifiers in an outdoor raceway pond with fluidized carriers. Water Sci. Technol., 82, 1081–1091. Keywords: high-ammonia-containing wastewater; immobilization; light-stress; microalgal-bacterial consortia; nitrification; photo-oxygenation Strain(s): 2173 DOI: 10.2166/wst.2020.226 Sekine, M., Yoshida, A., Akizuki, S., Kishi, M., Toda, T. 2020 Microalgae cultivation using undiluted anaerobic digestate by introducing aerobic nitrification–desulfurization treatment. Wat. Sci. Tech., 82, 1070-1080. Keywords: aerobic sludge; anaerobic digestion effluent; Chlorella sorokiniana; free ammonia inhibition; post anaerobic digestion treatment; simultaneous bacterial process Strain(s): 2173 DOI: 10.2166/wst.2020.153 Kimura, S., Yamada, T., Ban, S., Koyama, M., Toda, T. 2019 Nutrient removal from anaerobic digestion effluents of aquatic macrophytes with the green alga, Chlorella sorokiniana. Biochem. Eng. J., 142, 170-177. Keywords: Chlorella sorokiniana; anaerobic digestion effluent; maximum algal yield; nutrient removal rate; aquatic macrophytes Strain(s): 2173 DOI: 10.1016/j.bej.2018.12.001 Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151. Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion Strain(s): 94, 227, 2152, 2173, 2195, 2280 PubMed: 30658785 DOI: 10.1016/j.carbpol.2018.12.057 Hayashida, G., Schneider, C., Espíndola, L., Arias, D., Riquelme, C., Wulff-Zottele, C., Díaz-Palma, P., Rivas, M. 2017 Characterization of a Chlorophyta microalga isolated from a microbial mat in Salar de Atacama (northern Chile) as a potential source of compounds for biotechnological applications. Phycol. Res., 65, 202-211. Keywords: Altiplane; Chlorella sorokiniana; extreme tolerant; microalgae; microbial mat; oleaginous strain, polyunsaturated; fatty acid; stearic acid Strain(s): 2173 DOI: 10.1111/pre.12176 Huang, Y.-T. & Su, C.-P. 2014 High lipid content and productivity of microalgae cultivating under elevated carbon dioxide. Int. J. Environ. Sci. Technol., 11, 703-710. Keywords: Biodiesel; Chlorella; Global warming; Light intensity Strain(s): 2173 DOI: 10.1007/s13762-013-0251-y Hoshina, R. & Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132. Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept Strain(s): 2167, 2171, 2173, 2330 DOI: 10.1111/pre.12010 Mizuno, Y., Sato, A., Watanabe, K., Hirata, A., Takeshita, T., Ota, S., Sato, N., Zachleder, V., Tsuzuki, M., Kawano, S. 2013 Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species. Bioresour. Technol., 129, 150-155. Keywords: Sulfur deﬁciency; Chlorella spp.; Parachlorella kessleri; Starch; Lipids Strain(s): 2169, 2173 PubMed: 23238344 DOI: 10.1016/j.biortech.2012.11.030 Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184. Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection Strain(s): 2160, 2169, 2173, 2186, 4016 DOI: 10.1046/j.1529-8817.2003.02052. Tsuzuki, M. & Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104. Strain(s): 2093, 2138, 2160, 2173, 2235 DOI: 10.1016/0304-3770(89)90051-X Bürger, J., Miyachi, S., Galland, P., Senger, H. 1988 Quantum requirements of photosynthetic oxygen evolution and 77K fluorescence emission spectra in unicellular green algae grown under low- and high-CO₂-conditions. Botanica Acta, 101, 229-232. Strain(s): 2173, 2235 DOI: 10.1111/j.1438-8677.1988.tb00037.x Aizawa, K. & Miyachi, S. 1986 Carbonic anhydrase and CO₂ concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Lett., 39, 215-233. Keywords: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport Strain(s): 2093, 2160, 2173, 2235 DOI: 10.1111/j.1574-6968.1986.tb01860.x Miyachi, S., Tsuzuki, M., Maruyama, I., Ganter, M., Miyachi, S. 1986 Effects of CO₂ concentration during growth on the intracellular structure of Chlorella and Scenedesmus (Chlorophyta). J. Phycol., 22, 313-319. Keywords: Chlorella; CO2 concentration; electron microscopy; pyrenoid starch; Scenedesmus Strain(s): 687, 2157, 2160, 2170, 2173, 2280 Miyachi, S. & Miyachi, S. 1985 Ammonia induces starch degradation in Chlorella cells. Plant Cell Physiol., 26, 245-252. Keywords: ammonia effect; Chlorella; dark CO2 fixation; starch degradation; sucrose formation Strain(s): 687, 2157, 2160, 2170, 2173 DOI: 10.1093/oxfordjournals.pcp.a076903 Miyachi, S., Tsuzuki, M., Yagawa, Y. 1985 Carbonic anhydrase in various microalgae. In Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, Eds. by Lucas, W. J. & Berry, J. A., American Society of Plant Physiologists, Rockville, Md, pp. 145-154. Strain(s): 2093, 2160, 2173 Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240. Strain(s): 687, 2160, 2161, 2162, 2170, 2173, 2235, 2280 DOI: 10.1111/j.1749-6632.1984.tb12341.x Miyachi, S., Tsuzuki, M., Avramova, S. T. 1983 Utilization modes of inorganic carbon for photosynthesis in various species of Chlorella. Plant Cell Physiol., 24, 441-451. Keywords: bicarbonate fixation; carbonic anhydrase; Chlorella; photosynthetic CO2-fixation Strain(s): 687, 2093, 2157, 2160, 2173

Reference

Krivina, E., Portnov, A., Temraleeva, A. 2024 A description of Aliichlorella ignota gen. et sp. nov. and a comparison of the efficiency of species delimitation methods in the Chlorella-clade (Trebouxiophyceae, Chlorophyta). Phycol. Res., 72, 180-190.
Strain(s): 455, 2167, 2171, 2173, 2330, 4032, 4033, 4034
DOI: 10.1111/pre.12551

Ramírez‑Romero, A., Da Costa Magalhães, B., Matricon, L., Sassi, J.-F., Steyer, J.-P., Delrue, F. 2024 Aqueous phase recycling: impact on microalgal lipid accumulation and biomass quality. Environ. Sci. Pollut. Res., ,
Keywords: Renewable feedstock; Hydrothermal liquefaction; Green fuels; Microalgal biomass; Nutrient cycling
Strain(s): 227, 2173
PubMed: 38438644
DOI: 10.1007/s11356-024-32701-7

Nishi, K. 2023 Development of advanced light-tolerant microalgae-nitrifying bacteria consortia for ammonia removal under strong light irradiation using light-shielding hydrogel. PhD thesis, Soka University, Japan, 94 pp.
Strain(s): 2173

Marinič, D., Grilc, M., Hočevar, B., Delrue, F., Likozar, B. 2023 Liquefaction, cracking and hydrogenation of microalgae biomass resources to CO2 negative advanced biofuels: Mechanisms, reaction microkinetics and modelling. Renew. Energy, 203, 382-393.
Keywords: Microalgae; Biofuel; Liquefaction; Hydrodeoxygenation; One-pot reaction
Strain(s): 2173
DOI: 10.1016/j.renene.2022.12.055

Natori, N., Kuwata, M., Tanaka, K., Toda, T. 2023 Plankton survey method based on scanning electron microscopy. Bull. Plankton Soc. Japan, 70, 1-9 (in Japanese with English summary).
Keywords: whole effluent toxicity; drainage management; Raphidocelis subcapitata; johkasou
Strain(s): 2173
DOI: 10.24763/bpsj.70.1_1

Ramírez-Romero, A., Da Costa Magalhães, B., Dimitriades-Lemaire, A., Sassi, J.-F., Delrue, F., Steyer, J.-P. 2022 Chlorellaceae feedstock selection under balanced nutrient limitation. FERMENTATION, 8, 554 (article ID).
Keywords: biofuel; microalgal biomass; Chlorella; lipids
Strain(s): 227, 2173
DOI: 10.3390/fermentation8100554

Nishi, K., Akizuki, S., Toda, T., Matsuyama, T., Ida, J. 2022 Advanced light-tolerant microalgae-nitrifying bacteria consortia for stable ammonia removal under strong light irradiation using light-shielding hydrogel. Chemosphere, 297, 134252 (article ID).
Keywords: Microalgae–nitrifying bacteria consortia; Photoinhibition; Light-shielding; Free ammonia; Immobilisation
Strain(s): 2173
PubMed: 35271892
DOI: 10.1016/j.chemosphere.2022.134252

Qian, J., Shimotori, K., Liu, X., Ban, S., Akizuki, S., Fujiwara, M., Kodera, T., Toda, T., Imai, A. 2021 Enhancement of algal growth by Mg2+ released from anaerobic digestion effluent of aquatic macrophytes through photolysis. Biochem. Eng. J., 172, 108065 (article ID).
Keywords: Ultraviolet photolysis; DOM degradation; DOM molecular size distribution; Mg availability; Bio-digester effluent
Strain(s): 2173
DOI: 10.1016/j.bej.2021.108065

Sekine, M. 2021 Coupling process of simultaneous desulfurization–nitrification and microalgal cultivation for biogas and effluent from anaerobic digestion. PhD thesis, Soka University, Japan, 128 pp.
Strain(s): 2173

Kishi, M., Onouchi, H., Tanaka, K., Yoshida, A., Toda, T. 2021 Simultaneous microalgal culture and biogas upgrading using anaerobic digestion effluent. Bull. Plankton Eco-Eng. Res., 1, 58-73 (in Japanese with English summary).
Strain(s): 2173

Akizuki, S., Cuevas-Rodríguez, G., Toda, T. 2021 Nitrification of anaerobic digestate using a consortium of microalgae and nitrifiers in an open photobioreactor with moving bed carriers. Chemosphere, 263, 127948 (article ID).
Keywords: Bacterial immobilization; light attenuation; light stress; high-ammonia wastewater; photo-oxygenation
Strain(s): 2173
PubMed: 33297018
DOI: 10.1016/j.chemosphere.2020.127948

Akizuki, S., Kishi, M., Cuevas-Rodríguez, G., Toda, T. 2020 Effects of different light conditions on ammonium removal in a consortium of microalgae and partial nitrifying granules. Water Res., 171, 115445 (article ID).
Keywords: Energy-saving process; Photo-oxygenation; Short-cut nitrogen removal; Ammonium removal pathway; Empirical model
Strain(s): 2173
PubMed: 31954203
DOI: 10.1016/j.watres.2019.115445

Krivina, E. S. & Temraleeva, A. D. 2020 Identification problems and cryptic diversity of Chlorella-clade microalgae (Chlorophyta). Microbiology, 89, 720-732.
Keywords: microalgae; morphology; ecology; phylogeny; 18S−ITS1−5.8S−ITS2 fragments; polyphasic approach
Strain(s): 2167, 2173, 4032, 4034
DOI: 10.1134/S0026261720060107

Qian, J., Liu, X, Ban, S, Fujiwara, M, Kodera, T, Akizuki, S, Toda, T. 2020 pH treatments in continuous cultivation to maximize microalgal production and nutrient removal from anaerobic digestion effluent of aquatic macrophytes. J. Appl. Phycol., 32, 3349–3362.
Keywords: Biomass recovery; Flow-through cultivation; Nutrient removal; pH control; Mg availability; Bio-digester effluent
Strain(s): 2173
DOI: 10.1007/s10811-020-02196-z

Akizuki, S., Cuevas-Rodríguez, G., Toda, T. 2020 Anaerobic digestion effluent treatment using microalgae and nitrifiers in an outdoor raceway pond with fluidized carriers. Water Sci. Technol., 82, 1081–1091.
Keywords: high-ammonia-containing wastewater; immobilization; light-stress; microalgal-bacterial consortia; nitrification; photo-oxygenation
Strain(s): 2173
DOI: 10.2166/wst.2020.226

Sekine, M., Yoshida, A., Akizuki, S., Kishi, M., Toda, T. 2020 Microalgae cultivation using undiluted anaerobic digestate by introducing aerobic nitrification–desulfurization treatment. Wat. Sci. Tech., 82, 1070-1080.
Keywords: aerobic sludge; anaerobic digestion effluent; Chlorella sorokiniana; free ammonia inhibition; post anaerobic digestion treatment; simultaneous bacterial process
Strain(s): 2173
DOI: 10.2166/wst.2020.153

Kimura, S., Yamada, T., Ban, S., Koyama, M., Toda, T. 2019 Nutrient removal from anaerobic digestion effluents of aquatic macrophytes with the green alga, Chlorella sorokiniana. Biochem. Eng. J., 142, 170-177.
Keywords: Chlorella sorokiniana; anaerobic digestion effluent; maximum algal yield; nutrient removal rate; aquatic macrophytes
Strain(s): 2173
DOI: 10.1016/j.bej.2018.12.001

Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151.
Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion
Strain(s): 94, 227, 2152, 2173, 2195, 2280
PubMed: 30658785
DOI: 10.1016/j.carbpol.2018.12.057

Hayashida, G., Schneider, C., Espíndola, L., Arias, D., Riquelme, C., Wulff-Zottele, C., Díaz-Palma, P., Rivas, M. 2017 Characterization of a Chlorophyta microalga isolated from a microbial mat in Salar de Atacama (northern Chile) as a potential source of compounds for biotechnological applications. Phycol. Res., 65, 202-211.
Keywords: Altiplane; Chlorella sorokiniana; extreme tolerant; microalgae; microbial mat; oleaginous strain, polyunsaturated; fatty acid; stearic acid
Strain(s): 2173
DOI: 10.1111/pre.12176

Huang, Y.-T. & Su, C.-P. 2014 High lipid content and productivity of microalgae cultivating under elevated carbon dioxide. Int. J. Environ. Sci. Technol., 11, 703-710.
Keywords: Biodiesel; Chlorella; Global warming; Light intensity
Strain(s): 2173
DOI: 10.1007/s13762-013-0251-y

Hoshina, R. & Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132.
Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept
Strain(s): 2167, 2171, 2173, 2330
DOI: 10.1111/pre.12010

Mizuno, Y., Sato, A., Watanabe, K., Hirata, A., Takeshita, T., Ota, S., Sato, N., Zachleder, V., Tsuzuki, M., Kawano, S. 2013 Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species. Bioresour. Technol., 129, 150-155.
Keywords: Sulfur deﬁciency; Chlorella spp.; Parachlorella kessleri; Starch; Lipids
Strain(s): 2169, 2173
PubMed: 23238344
DOI: 10.1016/j.biortech.2012.11.030

Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184.
Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection
Strain(s): 2160, 2169, 2173, 2186, 4016
DOI: 10.1046/j.1529-8817.2003.02052.

Tsuzuki, M. & Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104.
Strain(s): 2093, 2138, 2160, 2173, 2235
DOI: 10.1016/0304-3770(89)90051-X

Bürger, J., Miyachi, S., Galland, P., Senger, H. 1988 Quantum requirements of photosynthetic oxygen evolution and 77K fluorescence emission spectra in unicellular green algae grown under low- and high-CO₂-conditions. Botanica Acta, 101, 229-232.
Strain(s): 2173, 2235
DOI: 10.1111/j.1438-8677.1988.tb00037.x

Aizawa, K. & Miyachi, S. 1986 Carbonic anhydrase and CO₂ concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Lett., 39, 215-233.
Keywords: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport
Strain(s): 2093, 2160, 2173, 2235
DOI: 10.1111/j.1574-6968.1986.tb01860.x

Miyachi, S., Tsuzuki, M., Maruyama, I., Ganter, M., Miyachi, S. 1986 Effects of CO₂ concentration during growth on the intracellular structure of Chlorella and Scenedesmus (Chlorophyta). J. Phycol., 22, 313-319.
Keywords: Chlorella; CO2 concentration; electron microscopy; pyrenoid starch; Scenedesmus
Strain(s): 687, 2157, 2160, 2170, 2173, 2280

Miyachi, S. & Miyachi, S. 1985 Ammonia induces starch degradation in Chlorella cells. Plant Cell Physiol., 26, 245-252.
Keywords: ammonia effect; Chlorella; dark CO2 fixation; starch degradation; sucrose formation
Strain(s): 687, 2157, 2160, 2170, 2173
DOI: 10.1093/oxfordjournals.pcp.a076903

Miyachi, S., Tsuzuki, M., Yagawa, Y. 1985 Carbonic anhydrase in various microalgae. In Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, Eds. by Lucas, W. J. & Berry, J. A., American Society of Plant Physiologists, Rockville, Md, pp. 145-154.
Strain(s): 2093, 2160, 2173

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 687, 2160, 2161, 2162, 2170, 2173, 2235, 2280
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Miyachi, S., Tsuzuki, M., Avramova, S. T. 1983 Utilization modes of inorganic carbon for photosynthesis in various species of Chlorella. Plant Cell Physiol., 24, 441-451.
Keywords: bicarbonate fixation; carbonic anhydrase; Chlorella; photosynthetic CO2-fixation
Strain(s): 687, 2093, 2157, 2160, 2173

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