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Strain Data
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| Strain number | NIES-836 | |||
|---|---|---|---|---|
| Phylum | Chlorophyta | |||
| Class | Trebouxiophyceae | |||
| Scientific name | Botryococcus braunii Kützing | |||
| Synonym | ||||
| Former name | ||||
| Common name | Green alga | |||
| Locality (Date of collection) | Imuta-ike Pond, Kagoshima, Japan (1997-06-**) | |||
| Latitude / Longitude | ||||
| Habitat (Isolation source) | Freshwater (Pond water) | |||
| History | < Mori, Fumi | |||
| Isolator (Date of isolation) | Mori, Fumi (1997-06-18) | |||
| Identified by | Mori, Fumi | |||
| State of strain | Cryopreservation; Unialgal; Clonal; Axenic[2014 Feb] | |||
|
Culture condition (Preculture condition) |
Medium:
AF-6
Temperature: 20 C Light intensity: 10-17 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 4 M |
|||
| Gene information | 18S rRNA ( AB780364 JQ941954 ) , rbcL ( JQ941955 ) | |||
| Cell size (min - max) | 7 - 11 μm | |||
| Organization | ||||
| Characteristics | oil (hydrocarbon) production (Race B) | |||
| Other strain no. | ||||
| Remarks | Cryopreserved; Axenic; Very slow growth | |||
| Movie | ||||
| Reference |
|---|
|
Maruyama, K. & Ohtsuki, T.
2024
A simple method for the preparation of single cells and regeneration of colonies of Botryococcus braunii NIES836.
J. Microbiol. Methods,
216,
106859 (article ID).
Keywords: Botryococcus braunii; Colony regeneration; Microalgae; Nutrient limitation; Single-cell preparation Strain(s): 836 PubMed: 37995829 DOI: 10.1016/j.mimet.2023.106859 Guionet, A., Oura, K., Akiyama, H., Hosano, H. 2019 The narrow window of energy application for oil extraction by arc discharge. J. Appl. Phycol., 31, 89–96. Keywords: Botryococcus braunii; Hydrocarbons; Biofuel; Matrix; Extraction Strain(s): 836 DOI: 10.1007/s10811-018-1579-y Wan, M., Zhang, Z., Wang, R., Bai, W., Huang, J., Wang, W., Shen, G., Yu, A., Li, Y. 2019 High-yield cultivation of Botryococcus braunii for biomass and hydrocarbons. Biomass Bioenerg., 131, 105399 (article ID). Keywords: Botryococcus braunii; Hydrocarbon; Heterotrophy; Photoautotrophy Strain(s): 836 DOI: 10.1016/j.biombioe.2019.105399 Guionet, A., Hosseini, B., Teissié, J., Akiyama, H., Hosseini, H. 2017 A new mechanism for efficient hydrocarbon electro-extraction from Botryococcus braunii. Biotechnol. Biofuels, 10, 39 (article ID). Keywords: Green energy; Biofuel; Hydrocarbon; Extraction; Botryococcus braunii kützing; Nanosecond pulsed electric field (nsPEF) Strain(s): 836 PubMed: 28228843 DOI: 10.1186/s13068-017-0724-1 Okumura, C., Saffreena, N., Rahman, M. A., Hasegawa, H., Miki, O., Takimoto, A. 2015 Economic efficiency of different light wavelengths and intensities using LEDs for the cultivation of green microalga Botryococcus braunii (NIES-836) for biofuel production. Environ. Prog. Sustain. Energy, 34, 269-275. Keywords: microalgae; Botryococcus braunii (NIES-836); growth efficiency; light-emitting diodes (LEDS); economic efficiency Strain(s): 836 DOI: 10.1002/ep.11951 Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319. Keywords: green alga; homospermidine; polyamine; thermospermine Strain(s): 18, 151, 160, 255, 296, 306, 360, 363, 640, 722, 836, 1409, 1410, 1411, 1419, 1837, 2164, 2199, 3374 PubMed: 24005181 DOI: 10.2323/jgam.59.313 Kim, B.-H., Ramanan, R., Cho, D.-H., Choi, G.-G., La, H.-J., Ahn, C.-Y., Oh, H.-M., Kim, H.-S. 2012 Simple, rapid and cost-effective method for high quality nucleic acids extraction from different strains of Botryococcus braunii. PLoS One, 7, e37770 (article ID). Strain(s): 836 PubMed: 22662217 DOI: 10.1371/journal.pone.0037770 Ranga Rao, A., Ravishankar, G. A., Sarada, R. 2012 Cultivation of green alga Botryococcus braunii in raceway, circular ponds under outdoor conditions and its growth, hydrocarbon production. Bioresour. Technol., 123, 528-533. Keywords: Microalgae; Botryococcus braunii; Outdoor cultivation; Hydrocarbon; Fatty acids Strain(s): 836 PubMed: 22940364 DOI: 10.1016/j.biortech.2012.07.009 Mogi, K., Fujii, T. 2012 Evaluation of ethanol toxicity to oil producing algae using a microfluidic device. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Okinawa, Japan 2012, pp. 857-859. Keywords: Microalgae; Botryococcus braunii; Toxicity assay; Vernier wall Strain(s): 836 Kawahara, K., Sagehashi, M., Fujii, T., Fujita, H., Hu, H.-Y., Sakoda, A. 2011 Potential of a green alga Botryococcus braunii for simultaneous water purification and biofuel production under open-air condition. J. Water Environ. Technol., 9, 29-37. Keywords: biofuel production; Botryococcus braunii; Microcystis aeruginosa; water purification Strain(s): 87, 836, 1090 DOI: 10.2965/jwet.2011.29 Toyoda, K., Gishi, M., Ihara, I. 2011 Effect of light quality and nutrients on growth of hydrocarbon-rich microalgae, Botryococcus braunii. In ISHS Acta Horticulturae 907: VI International Symposium on Light in Horticulture, pp. 255-258. Keywords: growth rate; phosphate; nitrogen; turbidity; ATR-FTIR Strain(s): 836 Li, Y., Qin, J. G. 2005 Comparison of growth and lipid content in three Botryococcus braunii strains. J. Appl. Phycol., 17, 551-556. Keywords: temperature; irradiance; salinity; Botryococcus braunii; hydrocarbon Strain(s): 836 DOI: 10.1007/s10811-005-9005-7 |
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