Strain number NIES-3371  
Phylum Heterokontophyta  
Class Bolidophyceae  
Scientific name Triparma eleuthera Ichinomiya & Lopes dos Santos  
Synonym  
Former name Bolidomonas pacifica L.Guillou & M.-J.Chrétiennot-Dinet var. eleuthera  
Common name  
Locality (Date of collection) Mediterranean Sea (Depth -25m), Malta (2008-07-08)  
Latitude / Longitude 35.66666666 / 14.1 
Habitat (Isolation source) Marine (Seawater)  
History < RCC (2013);  
Isolator (Date of isolation) Le Gall, F. (2008-07-08)  
Identified by Vaulot, Daniel (Identified by DNA analysis at RCC)  
State of strain Subculture; Unialgal; Non-clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESMMNK  
Temperature:  22 C
Light intensity:  24-35 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( LN735356 ) , 18S rRNA ( KF422629 ) , rbcL ( KR998422 ) , nadH1 ( KR998413 )  
Cell size (min - max) - 4 μm  
Organization  
Characteristics Planktonic ; Authentic strain (Ichinomiya et al. 2016) ; Non-siliceous flagellated stage[2018 May]  
Other strain no. Other collection strain no. : RCC2347; CCMP3396
Other strain no. : BOUM117-22  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Kuwata, A., Yamada, K., Ichinomiya, M., Yoshikawa, S., Tragin, M., Vaulot, D., Lopes dos Santos, A. 2018 Bolidophyceae, a sister picoplanktonic group of diatoms – a review. Front. Mar. Sci., 5, 370 (article ID).
Keywords: bolidophyceae; parmales; diatoms; genetic diversity; mitosis; geographical distribution; seasonal dynamics and silicification
Strain(s): 25652682336933703371369937003701 
DOI: 10.3389/fmars.2018.00370

Ichinomiya, M., Lopes dos Santos, A., Gourvil, P., Yoshikawa, S., Kamiya, M., Ohki, K., Audic, S., De Vargas, C., Noël, M. H., Vaulot, D., Kuwata, A. 2016 Diversity and oceanic distribution of the Parmales (Bolidophyceae), a picoplanktonic group closely related to diatoms. ISME J., 10, 2419-2434.
Strain(s): 25652682336933703371369937003701 
PubMed: 27003244
DOI: 10.1038/ismej.2016.38

del Campo, J., Sieracki, M.E., Molestina, R., Keeling, P., Massana, R., Ruiz-Trillo, I. 2014 The others: our biased perspective of eukaryotic genomes. Trends Ecol. Evol., 29, 252-259.
Strain(s): 1000258225842587259026702673269126932694269526962697269927002707275127532755275627582761336933703371 
PubMed: 24726347
DOI: 10.1016/j.tree.2014.03.006

Guillou, L. 2011 Characterization of the Parmales: much more than the resolution of a taxonomic enigma. J. Phycol., 47, 2-4.
Strain(s): 2682336933703371 
DOI: 10.1111/j.1529-8817.2010.00951.x

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