Strain number NIES-2740  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox kirkiorum H.Nozaki, H.Kawai-Toyooka & N.Isaka  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Hajima, Gifu, Japan (2006-07-27)  
Latitude / Longitude 35.253 / 136.67444444 
Habitat (Isolation source) Freshwater  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2006-07-28)  
Identified by Isaka, Nanako; Matsuzaki, Ryo; Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  AF-6VTAC  
Temperature:  20 C
Light intensity:  12-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-26S ( AB663324 ) , rbcL ( AB663322 ) , psbC ( AB663323 ) , MID ( LC699264 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Phototaxis ; Authentic strain (Isaka 2012) ; Homothallic ; Monoecious ; Oogamy ; Resting zygote forming  
Other strain no. Other collection strain no. : UTEX 2966
Other strain no. : 2006-728-2-B8  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Yamagishi, S., Yamamoto, K., Takahashi, K., Kawai-Toyooka, H., Suzuki, S., Matsuzaki, R., Yamaguchi, H., Kawachi, M., Higashiyama, T., Nozaki, H. 2023 Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect. Volvox (Chlorophyceae). Phycol. Res., 72, 46-55.
Keywords: male gamete; mating system; minus dominance gene (MID); molecular evolution; sex; sperm packet; volvocine green algae
Strain(s): 28945646054154454556957257458265265365473285185285787289189213621363170817101859245725692735273627403382338337803782378638743984398639884021402543364432453545364537 
DOI: 10.1111/pre.12538

Nozaki, H., Mahakham, W., Heman, W., Matsuzaki, R., Kawachi, M. 2020 A new preferentially outcrossing monoicous species of Volvox sect. Volvox (Chlorophyta) from Thailand. PLoS One, 15, e0235622 (article ID).
Strain(s): 273627403382338338744021433644324433443444354436 
PubMed: 32614898
DOI: 10.1371/journal.pone.0235622

Isaka, N., Kawai-Toyooka, H., Matsuzaki, R., Nakada, T., Nozaki, H. 2012 Description of two new monoecious species of Volvox sect. Volvox (Volvocaceae, Chlorophyceae), based on comparative morphology and molecular phylogeny of cultured material. J. Phycol., 48, 759-767.
Keywords: Chlorophyceae; cultures; molecular phylogeny; morphology; taxonomy; Volvocaceae; Volvox ferrisii sp. nov.; Volvox kirkiorum sp. nov.; Volvox sect. Volvox
Strain(s): 54327362737273827392740 
PubMed: 27011093
DOI: 10.1111/j.1529-8817.2012.01142.x

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