Strain number NIES-969  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Synechococcus sp.  
Synonym  
Former name  
Common name Blue-green alga ; Cyanobacteria ; Picoplankton  
Locality (Date of collection) East China Sea (1998-06-01)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Seawater)  
History < Tezuka, Naoaki  
Isolator (Date of isolation) Tezuka, Naoaki (1998-09-**)  
Identified by Tezuka, Naoaki  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2014 Mar]  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  9-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( AF448060 LC455657 ) , 23S rRNA ( LC455658 ) , rpoC1 ( AF448082 ) , petB ( LC716333 )  
Cell size (min - max) 1 - 2 μm  
Organization Unicellular 
Characteristics Picoplankton ; SC5.1, VIc (Suzuki et al. 2022)  
Other strain no. Other strain no. : 1002  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Ota, S., Fuchida, S., Yamaguchi, H., Yamagishi, T., Yamamoto, H., Koshikawa, H., Kawachi, M. 2022 Propidium iodide staining and flow cytometry-based assessment of heavy metal impact on marine phytoplankton. Cytologia, 87, 177-187.
Keywords: Cyanobacteria; Deep-sea mining; Flow cytometry; Heavy metal contaminant; Microalgae; On-chip sort
Strain(s): 9699812670 
DOI: 10.1508/cytologia.87.177

Suzuki, S., Yamaguchi, H., Kawachi, M. 2022 Potential genetic diversity of Synechococcus-related strains maintained in the Microbial Culture Collection of the National Institute for Environmental Studies, Japan. Microb. Resour. Syst., 38, 63-74.
Keywords: Synechococcus; Cyanobium; picoplankton; molecular marker
Strain(s): 93793893994094194294394594694794995095195295395495595695795895996096196296396496596997097197297397497597697797897998198298398498598698798813411342134313441345134613471348 

Ota, S., Yamaguchi, H., Vanel, F., Fuchida, S., Koshikawa, H., Yamagishi, T., Yamamoto, H., Kawachi, M. 2020 Differential heavy metal sensitivity in seven algal species from the NIES culture collection based on delayed fluorescence assays. Phycol. Res., 68, 41-49.
Strain(s): 9699719811001131025872670 
DOI: 10.1111/pre.12403

Yamagishi, T., Ota, S., Yamaguchi, H., Koshikawa, H., Tatarazako, N., Yamamoto, H., Kawachi, M. 2019 Ecotoxicological bioassay using marine algae for deep-sea mining. In Environmental Issues of Deep-Sea Mining: Impacts, Consequences and Policy Perspectives, Ed. by Sharma, R., Springer, Cham, pp. 255-271.
Strain(s): 323613969971981100010011310141123042587259026702672 
DOI: 10.1007/978-3-030-12696-4_10

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