Strain number NIES-3386  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Colemanosphaera charkowiensis (Korshikov) H.Nozaki, T.K.Yamada, F.Takahashi, R.Matsuzaki & T.Nakada  
Synonym Pandorina charkowiensis Korshikov  
Former name  
Common name Green alga  
Locality (Date of collection) Isanuma Pond, Kawagoe, Saitama, Japan (2013-06-15)  
Latitude / Longitude 35.92138888 / 139.51444444 
Habitat (Isolation source) Freshwater (Ditch water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2010-06-15)  
Identified by Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  AF-6VTAC  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( AB905582 )  
Cell size (min - max)  
Organization  
Characteristics Planktonic ; Dioecious ; Anisogamous (Nozaki et al. 2014) ; Resting spore-forming  
Other strain no. Other strain no. : 2013-0615-IC-3  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Nozaki, H., Yamada, K. T., Takahashi, F., Matsuzaki, R., Nakada, T. 2014 New "missing link" genus of the colonial volvocine green algae gives insights into the evolution of oogamy. BMC Evol. Biol., 14, 37 (article ID).
Strain(s): 3382338333843385338633873388 
PubMed: 24589311
DOI: 10.1186/1471-2148-14-37

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