Strain number NIES-4338  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox rousseletii G.S.West  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Lake Sagami, Kanagawa, Japan (2015-06-10)  
Latitude / Longitude 35.61041 / 139.18583 
Habitat (Isolation source) Freshwater (Reservoir water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2015-05-10)  
Identified by Kinbara, Ryosuke; Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Axenic[2022 Mar]  
Culture condition
(Preculture condition)
Medium:  AF-6/3AF-6  
Temperature:  20 C
Light intensity:  24-26 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( LC493799 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Phototaxis ; Dioecious ; Heterothallic ; Female  
Other strain no. Other strain no. : 2015-0610-3v9  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Kimbara, R., Isaka, N., Matsuzaki, R., Kawai-Toyooka, H., Kawachi, M., Nozaki, H. 2019 Morphological and molecular identification of the dioecious “African species Volvox rousseletii (Chlorophyceae) in the water column of a Japanese lake based on field-collected and cultured materials. PLoS One, 14, e0221632 (article ID).
Strain(s): 4336433743384339434043414342 
PubMed: 31465476
DOI: 10.1371/journal.pone.0221632

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