Strain number NIES-2163  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Auxenochlorella protothecoides (Krüger) Kalina & Puncochárová  
Synonym Chlorella protothecoides Krüger  
Former name Chlorella vulgaris Beijerinck  
Common name Green alga  
Locality (Date of collection)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < BIU (UTEX);  
Isolator (Date of isolation) Pringsheim, E. G.  
Identified by Kessler, E.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  Pro (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( KP645219 )  
Cell size (min - max) 3 - 12 μm  
Organization Unicellular 
Characteristics  
Other strain no. Other collection strain no. : IAM C-150; SAG 211-11a; UTEX 29; ATCC 30581; ATCC 13482; CCAP 211/11a; (BIU 29)
 
Remarks Cryopreserved; Axenic 
Movie  
Reference
Hamana, K., Furuchi, T., Hayashi, H., Uemura, T., Niitsu, M. 2023 Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta. ─Polyamine analysis of algae V─ Microb. Resour. Syst., 39, 33-40.
Keywords: alga; Chlorophyta; Glaucophyta; polyamine; Rhodophyta
Strain(s): 22625025455055162612981332170318362137216321642165216622522353289433773645386738913892410743904452 

Chu, C. Y., Liao, W. R., Huang, R., Lin, L. P. 2004 Haemagglutinating and antibiotic activities of freshwater microalgae. World J. Microbiol. Biotechnol., 20, 817–825.
Keywords: Antibiotic activity; erythrocytes; freshwater microalgae; haemagglutination
Strain(s): 22622721592163216521692175218922082211221322142229223122332240224122432247224832773284 
DOI: 10.1007/s11274-004-8712-6

Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553.
Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology
Strain(s): 6866872150215121602163216921752176 
DOI: 10.1111/j.0022-3646.1992.00550.x

Higashiyama, T. & Yamada, T. 1991 Electrophoretic karyotyping and chromosomal gene mapping of Chlorella. Nucleic Acids Res., 19, 6191-6195.
Strain(s): 2163216621712189 
PubMed: 1956777
DOI: 10.1093/nar/19.22.6191

Koga, T., Arasaki, T., Tanaka, T., Ueda, S. 1991 Studies on cell fusion of microalgae, part 2 protoplast induction and regeneration of Chlorella. Annu. Rep. Kumamoto Kogyo Univ., 17, 279-286.
Strain(s): 68521632189 

Koga, T., Arasaki, T., Tanaka, T., Ueda, S. 1991 Studies on cell fusion of microalgae, part 3 interspecific cell fusion of Chlorella. Annu. Rep. Kumamoto Kogyo Univ., 17, 287-293.
Strain(s): 68521632189 

Huss, V. A. R., Wein, K. H., Kessler, E. 1988 Deoxyribonucleic acid reassociation in the taxonomy of the genus Chlorella. Arch. Mikrobiol., 150, 509-511.
Strain(s): 2163 

Yamada, T. & Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 2276862159216321662168217121892352 
DOI: 10.1007/BF00690809

Maruyama, K. 1977 Classification of Chlorella strain by infrared absorption spectra of cell samples. Bot. Mag. Tokyo, 90, 67-77.
Strain(s): 2262276856872151216321702175 
DOI: 10.1007/BF02489470

Maruyama, K. 1977 Classification of Chlorella strains by cell appearance and group sera. Bot. Mag. Tokyo, 90, 57-66.
Strain(s): 2262276852151216321702175 
DOI: 10.1007/BF02489469

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