Strain number NIES-1269  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella vulgaris Beijerinck  
Synonym  
Former name Formerly identified as Chlorella sp., re-identified at NIES by DNA analysis  
Common name Green alga  
Locality (Date of collection) Banzu Tidal Flat, Kisarazu, Chiba, Japan (2002-05-14)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Tidal flat water)  
History < Moriya, Mayumi  
Isolator (Date of isolation) Moriya, Mayumi (2002-06-18)  
Identified by Re-identified by NIES  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  22-32 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB488579 )  
Cell size (min - max) 10 - 15 μm  
Organization Unicellular 
Characteristics  
Other strain no. Other strain no. : M-56  
Remarks Cryopreserved 
Movie  
Reference
Purba, L. D. A., Othman, A., Yuzir, A., Mohamad, S. V., Iwamoto, K., Abdullah, N., Shimizu, K., Hermana, J. 2022 Enhanced cultivation and lipid production of isolated microalgae strains using municipal wastewater. Environ. Technol. Innov., 27, 102444 (article ID).
Keywords: Microalgae; Municipal wastewater; Nutrient removal; Lipid production
Strain(s): 1269 
DOI: 10.1016/j.eti.2022.102444

Othman, F. S., Jamaluddin, H., Ibrahim, Z., Hara, H., Yahya, N. A., Iwamoto, K., Mohamad, S. E. 2019 Production of α-linolenic acid by an oleaginous green algae Acutodesmus obliquus isolated from Malaysia. J. Pure Appl. Microbiol., 13, 1297-1306.
Keywords: Acutodesmus obliquus; Chlorella sp.; Chlorella vulgaris; Lipid; Malaysia
Strain(s): 1269 
DOI: 10.22207/JPAM.13.3.01

Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939
Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat
Strain(s): 64164268612692170 
PubMed: 24931503
DOI: 10.4014/jmb.1405.05047

Sharifah, E. N. & Eguchi, M. 2012 Benefits of live phytoplankton, Chlorella vulgaris, as a biocontrol agent against fish pathogen Vibrio anguillarum. Fish. Sci., 78, 367-373.
Keywords: Chlorella vulgaris; Vibrio anguillarum; Biocontrol; Sulfitobacter sp.
Strain(s): 1269 
DOI: 10.1007/s12562-011-0465-1

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