Strain number NIES-2238  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas reinhardtii P.A.Dangeard  
Synonym Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
Former name  
Common name Green alga  
Locality (Date of collection) near Amherst, Massachusetts, USA  
Latitude / Longitude 42.364125 /  
Habitat (Isolation source) Terrestrial (Soil)  
History < IAM (2007) < Kuchitsu, Kazuyuki (1991) < Mihara, S. < Matsuda, Y. < Togasaki, R.  
Isolator (Date of isolation) Smith, G. M. (1945-**-**)  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  TAP (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB511835 AB701553 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Mating type (+) ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005)  
Other strain no. Other collection strain no. : IAM C-541; CCAP 11/32C; SAG 34.89; UTEX 2244; UTCC 11; CGC CC-125 mt+
Other strain no. : 137c mt+  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Karitani, Y., Yamada, R., MatsumotoT., Ogino, H. 2024 UV mutagenesis improves growth potential of green algae in a green algae–yeast co‑culture system. Arch. Microbiol., 206, 61 (article ID).
Keywords: Chlamydomonas reinhardtii; Co-cultivation; Photosynthesis; Saccharomyces cerevisiae; Mutagenesis; Transcriptome analysis
Strain(s): 2238 
PubMed: 38216809
DOI: 10.1007/s00203-023-03796-2

Shimizu, I., Yamashita, K., Tokunaga, E. 2022 Development of a simple fabrication method for magnetic micro stir bars and induction of rotational motion in Chlamydomonas reinhardtii. Micromachines, 13, 1842 (article ID).
Keywords: magnetic micro stirrer bar; microchip; motion analysis; microalga; Chlamydomonas; neodymium magnet; flagella; orbital motion
Strain(s): 2238 
PubMed: 36363863
DOI: 10.3390/mi13111842

Toyoshima, H., Miyata, A., Yoshida, R., Ishige, T., Takaichi, S., Kawasaki, S. 2021 Distribution of the water-soluble astaxanthin binding carotenoprotein (AstaP) in Scenedesmaceae. Mar. Drugs, 19, 349 (article ID).
Keywords: astaxanthin; Scenedesmus; Coelastrella; carotenoprotein; astaxanthin-binding protein
Strain(s): 22382540 
PubMed: 34203079
DOI: 10.3390/md19060349

Yamashita, K., Tokunaga, E. 2020 Noninvasive and safe cell viability assay for Paramecium using natural pigment extracted from food. Sci Rep, 10, 10996 (article ID).
Strain(s): 2238 
PubMed: 32620770
DOI: 10.1038/s41598-020-67712-0

Isono, T., Yamashita, K., Momose, D., Kobayashi, H., Kitamura, M., Nishiyama, Y., Hosoya, T., Kanda, H., Kudo, A., Okada, N., Yagi, T., Nakata, K., Mineki, S., Tokunaga, E. 2015 Scan-free absorbance spectral imaging A(x, y, λ) of single live algal cells for quantifying absorbance of cell suspensions. PLoS One, 10, e0128002 (article ID).
Strain(s): 2238 
PubMed: 26061268
DOI: 10.1371/journal.pone.0128002

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Pröschold, T., Harris, E. H., Coleman, A. W. 2005 Portrait of a species: Chlamydomonas reinhardtii. Genetics, 170, 1601-1610.
Keywords: Chlamydomonas; Internal Transcribed Spacer; phylogenetics; population genetics; ribosomal cistrons
Strain(s): 22372238 
PubMed: 15956662
DOI: 10.1534/genetics.105.044503

Matsuda, Y., Shimada, T., Sakamoto, Y. 1992 Ammonium ions control gametic differentiation and dedifferentiation in Chlamydomonas reinhardtii. Plant Cell Physiol., 33, 909-914.
Keywords: cell cycle progression; Chlamydomonas reinhardtii; NH4+ starvation; organic nitrogen compounds; sexual reproduction
Strain(s): 2238 
DOI: 10.1093/oxfordjournals.pcp.a078340

Kimpel, D. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii I. Localization. Plant Cell Physiol., 24, 255-259.
Keywords: carbonic anhydrase; cell wall-less mutant; Chyamydomonas reinhardtii; periplasm; wall lysing enzyme
Strain(s): 22372238 
DOI: 10.1093/pcp/24.2.255

Spencer, K. G., Kimpel, D. L., Fisher, M. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii II. Requirements for carbonic anhydrase induction. Plant Cell Physiol., 24, 301-304.
Keywords: carbonic anhydrase; Chlamydomonas reinhardtii; enzyme induction; photosynthesis; photosynthetic mutants
Strain(s): 2238 
DOI: 10.1093/pcp/24.2.301

Sakaguchi, T., Horikoshi, T., Nakajima, A. 1978 Uptake of uranium from seawater by microalgae. J. Ferm. Technol., 56, 561-565.
Strain(s): 21382139221222382279 

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