Strain number NIES-2236  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas reinhardtii P.A.Dangeard  
Synonym Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
Former name  
Common name Green alga  
Locality (Date of collection) near Amherst, Massachusetts, USA  
Latitude / Longitude 42.364125 /  
Habitat (Isolation source) Terrestrial (Soil)  
History < IAM (2007) < BIU (UTEX; 1967) < CCAP (1952);  
Isolator (Date of isolation) Smith, G. M. (1945-**-**)  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  2-3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information 18S rRNA ( AB511834 AB701551 ) , rbcL ( AB511845 )  
Cell size (min - max) 4 - 10 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Mating type (+)  
Other strain no. Other collection strain no. : IAM C-239; CCAP 11/32A; SAG 11-32b; UTEX 90; NIBB 4014; CGC CC-1010; CGC CC-409
Other strain no. : 137c mt+  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Hosoda, K., Seno, S., MurakamiN., Matsuda, H., Osada, Y., Kamiura, R., Kondoh, M. 2024 Synthetic model ecosystem of 12 cryopreservable microbial species allowing for a noninvasive approach. Biosystems, 235, 105087 (article ID).
Keywords: Synthetic ecosystem; Experimental model ecosystem; Microbial microcosm; Machine learning
Strain(s): 21352272236 
PubMed: 37989470
DOI: 10.1016/j.biosystems.2023.105087

Flores, D. C., Fricke, M., Wesp, V., Desirò, D., Kniewasser, A., Hölzer, M., Marz, M., Mittag, M. 2021 A marine Chlamydomonas sp. emerging as an algal model. J. Phycol., 57, 54-69.
Strain(s): 882220222042207221122182220222322292236224122422248 
PubMed: 33043442
DOI: 10.1111/jpy.13083

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Nakazawa, A. & Nishii, I. 2012 Amidic and acetonic cryoprotectants improve cryopreservation of volvocine green algae. CryoLetters, 33, 201-212.
Keywords: cryopreservation; dimethylformamide; hydroxyacetone; Volvocaceae; Volvox; Chlamydomonas
Strain(s): 4184685455645747177217287297307311331136117102236 
PubMed: 22825787

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