Strain number NIES-3576  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Nitzschia sp.  
Synonym  
Former name  
Common name Pennate diatom  
Locality (Date of collection) Miyara River, Ishigaki, Okinawa, Japan (2012-03-06)  
Latitude / Longitude  
Habitat (Isolation source) Brackish water (Plant (Mangrove))  
History < Nakayama, Takeshi < Kamikawa, Ryoma < Taira, Misaka  
Isolator (Date of isolation) Taira, Misaka (2012-03-**)  
Identified by Mayama, Shigeki  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  ESM + CB-V  
Temperature:  20 C
Light intensity:  0 µmol photons/m2/sec,
Duration:  21 D  
Gene information Mitochondrial DNA ( AP018509 ) , Plastid DNA ( AP018508 ) , 16S rRNA ( AB899716 ) , 28S rRNA ( AB899702 )  
Cell size (min - max) - 20 μm  
Organization Unicellular 
Characteristics Heterotrophic ; Benthic ; Nonphotosynthetic plastids ; Genome decoded strain (Kamikawa et al. 2018)  
Other strain no. Other strain no. : A4  
Remarks Cryopreserved; Axenic; Nonphotosynthetic plastids 
Movie  
Reference
Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62.
Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine
Strain(s): 1247482532742762772822863043313813874636236246997067157411443214521462149235123782433253525842588263526772716272828603576374540604109428043914392 

Kamikawa, R., Azuma, T., Ishii, K-I., Matsuno, Y., Miyashita, H. 2018 Diversity of organellar genomes in non-photosynthetic diatoms. Protist, 169, 351-361.
Keywords: ATP synthase complex; mitochondria; Nitzschia; plastids; vitamin B1
Strain(s): 272935763581 
PubMed: 29803116
DOI: 10.1016/j.protis.2018.04.009

Kamikawa, R., Yubuki, N., Yoshida, M., Taira, M., Nakamura, N., Ishida, K-I., Leander, B. S., Miyashita, H., Hashimoto, T., Mayama, S., Inagaki, Y. 2015 Multiple losses of photosynthesis in Nitzschia (Bacillariophyceae). Phycol. Res., 63, 19-28.
Keywords: apochlorotic diatoms; genetic diversity; large subunit rRNA; molecular phylogenetic analysis; nonphotosynthetic plastids; plastid 16S rRNA
Strain(s): 357635773578357935803581 
DOI: 10.1111/pre.12072

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