Strain number NIES-4284  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chloromonas muramotoi Matsuzaki, Nozaki & Kawachi  
Synonym  
Former name  
Common name Green alga ; Snow alga  
Locality (Date of collection) the Hakkoda Botanical Garden of Tohoku University, Mt. Hakkoda, Aomori, Aomori, Japan (2012-05-16)  
Latitude / Longitude 40.646944 / 140.851389 
Habitat (Isolation source) Terrestrial (Snow)  
History < Matsuzaki, Ryo  
Isolator (Date of isolation) Matsuzaki, Ryo (2012-03-23)  
Identified by Matsuzaki, Ryo  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  5 C
Light intensity:  51 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( LC438435 ) , 28S rRNA ( LC438439 ) , atpB ( LC438443 ) , psaB ( LC438447 ) , rbcL ( LC438451 ) , ITS2 ( LC438455 )  
Cell size (min - max) 12 - 19 μm  
Organization Unicellular; Flagellate 
Characteristics Cryophilic ; Authentic strain (Matsuzaki et al. 2019)  
Other strain no. Other strain no. : HkCl-57  
Remarks  
Movie  
Reference
Matsuzaki, R., Suzuki, S., Yamaguchi, H., Kawachi, M., Kanesaki, Y., Yoshikawa, H., Mori, T., Nozaki, H. 2021 The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid. BMC Ecol. Evol., 21, 11 (article ID).
Keywords: Chloromonas; Evolution; Green algae; Hydrophobicity of RBCS helices; Pyrenoid; Pyrenoid loss; Rubisco small subunit (RBCS)
Strain(s): 14442442522072212221522422243249937534284 
PubMed: 33514317
DOI: 10.1186/s12862-020-01733-1

Matsuzaki, R., Nozaki, H., Takeuchi, N., Hara, Y., Kawachi, M. 2019 Taxonomic re-examination of “Chloromonas nivalis (Volvocales, Chlorophyceae) zygotes” from Japan and description of C. muramotoi sp. nov. PLoS One, 14, e0210986 (article ID).
Strain(s): 46423792380256328623389370737534284 
PubMed: 30677063
DOI: 10.1371/journal.pone.0210986

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