Strain number NIES-4018  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Eudorina sp.  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Refer to NIES-2734 and NIES-2735  
Latitude / Longitude  
Habitat (Isolation source) Freshwater  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2010-06-23)  
Identified by Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information mat3 ( AB771936 AB771937 )  
Cell size (min - max) 6 - 22 μm  
Organization Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Phototaxis ; F1 clone of NIES-2734 x 2735 ; Heterothallic ; Anisogamous ; Mating type (female) ; Haploid life cycle ; Resting zygote forming  
Other strain no. Other strain no. : 2010-623-F1-E8  
Remarks Cryopreserved; Axenic 
Movie  
Reference
Hamaji, T., Kawai-Toyooka, H., Uchimura, H., Suzuki, M., Noguchi, H., Minakuchi, Y., Toyoda, A., Fujiyama, A., Miyagishima, S., Umen, J. G., Nozaki, H. 2018 Anisogamy evolved with a reduced sexdetermining region in volvocine green algae. Communications Biology, 1, 17 (article ID).
Strain(s): 398239833984398540184100 
DOI: 10.1038/s42003-018-0019-5

Yamashita, S., Arakaki, Y., Kawai-Toyooka, H., Noga, A., Hirono, M., Nozaki, H. 2016 Alternative evolution of a spheroidal colony in volvocine algae: developmental analysis of embryogenesis in Astrephomene (Volvocales, Chlorophyta). BMC Evol. Biol., 16, 243 (article ID).
Strain(s): 40174018 
PubMed: 27829356
DOI: 10.1186/s12862-016-0794-x

Hiraide, R., Kawai-Toyooka, H., Hamaji, T ., Matsuzaki, R., Kawafune, K., Abe, J., Sekimoto, H., Umen, J., Nozaki, H. 2013 The evolution of male–female sexual dimorphism predates the gender-based divergence of the mating locus gene MAT3/RB. Mol. Biol. Evol., 30, 1038-1040.
Keywords: gender-based divergence; gene conversion; male–female sexual dimorphism; MAT3/RB, mating type locus; volvocine algae
Strain(s): 722733866171017111859186139854018 
PubMed: 23364323
DOI: 10.1093/molbev/mst018

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