Strain number NIES-2586  
Phylum Heterokontophyta  
Class Chrysophyceae  
Scientific name Picophagus flagellatus Guillou & Chrétiennot-Dinet  
Synonym  
Former name  
Common name Picoplankton  
Locality (Date of collection) Equatorial Pacific Ocean (Depth -15m), Kiribati (1994-11-07)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Seawater)  
History < RCC (2009);  
Isolator (Date of isolation) Vaulot, Daniel (1994-11-07)  
Identified by Guillou, L.  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM + Rice  
Temperature:  20 C
Light intensity:  15-20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AF185051 )  
Cell size (min - max) - 2 μm  
Organization  
Characteristics Authentic strain (Guillou et al. 1999) ; Heterotrophic ; Phagotrophic ; Planktonic  
Other strain no. Other collection strain no. : RCC22; CCMP1953
Other strain no. : OLI11SC2D  
Remarks Cryopreserved 
Movie  
Reference
Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 825427428632433135338149462362499710121013101513201330133313341335137213771388139013961397140814381439144118681964200821422144214923002305232524332498250225662584258625892590267728783356337437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Fuller, N. J., Campbell, C., Allen, D. J., Pitt, F. D., Le Gall, F., Vaulot, D., Scanlan, D. J. 2006 Analysis of photosynthetic picoeukaryote diversity at open ocean sites in the Arabian Sea using a PCR biased towards marine algal plastids. Aquat. Microb. Ecol., 43, 79-93.
Keywords: Photosynthetic picoeukaryotes; Plastid 16S rRNA; Arabian Sea; PCR
Strain(s): 25862588258926722673268726902693 
DOI: 10.3354/ame043079

Guillou, L., Jacquet, S., Chrétiennot-Dinet, M. J., Vaulot, D. 2001 Grazing impact of two small heterotrophic flagellates on Prochlorococcus and Synechococcus. Aquat. Microb. Ecol., 26, 201-207.
Strain(s): 25862589 
DOI: 10.3354/ame026201

Guillou, L., Chrétiennot-Dinet, M. J., Boulben, S., Moon-van der Staay, S. Y., Vaulot, D. 1999 Symbiomonas scintillans gen. et sp. nov. and Picophagus flagellatus gen. et sp. nov. (Heterokonta): two new heterotrophic flagellates of picoplanktonic size. Protist, 150, 383-398.
Strain(s): 25862589 
DOI: 10.1016/S1434-4610(99)70040-4

Guillou, L., Moon-van der Staay, S. Y., Claustre, H., Partensky, F., Vaulot, D. 1999 Diversity and abundance of Bolidophyceae (Heterokonta) in two oceanic regions. Appl. Environ. Microbiol., 65, 4528-4536.
Strain(s): 2586258926822691 
PubMed: 10508085
DOI: 10.1128/AEM.65.10.4528-4536.1999

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