Strain number NIES-439  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Gungnir neglectum (Pascher) Nakada  
Synonym Chlamydomonas neglecta Korshikov ex Pascher; Chlorogonium neglectum Pascher  
Former name  
Common name Green alga  
Locality (Date of collection) Tsukuba, Ibaraki, Japan (1984-05-08)  
Latitude / Longitude 36.104284 / 140.089918 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1984-05-26)  
Identified by Suda, Shoichiro; Nozaki, Hisayoshi; Nakada, Takashi (Reidentify)  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB360745 ) , atpB ( AB084326 ) , psaB ( AB084366 ) , rbcL ( AB010243 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Authentic strain ; Type specimen (NIES-50006, Epitype) ; Prey for NIES-2497 and NIES-2498  
Other strain no. Other strain no. : T-4-19  
Remarks Axenic 
Movie  
Reference
Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 12314442142843243944656756957165686188796810482293256441244125 
PubMed: 26595722
DOI: 10.1111/jeu.12283

Nakada, T., Nozaki, H., Pröschold, T. 2008 Molecular phylogeny, ultrastructure, and taxonomic revision of Chlorogonium (Chlorophyta): emendation of Chlorogonium and description of Gungnir gen. nov. and Rusalka gen.nov. J. Phycol., 44, 751-760.
Keywords: Chlorogonium; Gungnir gen.nov.; molecular phylogeny; morphology; Rusalka gen.nov.; taxonomy; ultrastructure; Volvocales
Strain(s): 12343969275175576113571359136018694125 
DOI: 10.1111/j.1529-8817.2008.00525.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968 
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Ishida, K. & Murakami, M. 2000 Kasumigamide, an antialgal peptide from the cyanobacterium Microcystis aeruginosa. J. Org. Chem., 65, 5898-5900.
Strain(s): 87439 
PubMed: 10987919
DOI: 10.1021/jo991918f

Nozaki, H., Ohta, N., Morita, E., Watanabe, M. M. 1998 Toward a natural system of species in Chlorogonium (Volvocales, Chlorophyta): a combined analysis of morphological and rbcL gene sequence data. J. Phycol., 34, 1024-1037.
Keywords: Chlorogonium; Chlorogonium kasakii sp. nov.; Chlorophyta; molecular phylogeny; monophyletic species; morphology; natural systematics; rbcL gene; species taxonomy; Volvocales
Strain(s): 123439446692742743744745746747748749750751752753754755756757758759760761 
DOI: 10.1046/j.1529-8817.1998.341024.x

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