Strain number NIES-854  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Astrephomene gubernaculifera Pocock  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Monroe County, Indiana, USA (1962-10-**)  
Latitude / Longitude  
Habitat (Isolation source) (Soil)  
History < Nozaki, Hisayoshi < UTEX  
Isolator (Date of isolation) Brooks, A. E. (1962-10-**)  
Identified by Brooks, A. E.  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  VTAC  
Temperature:  20 C
Light intensity:  7-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D  
Gene information atpB ( AB044181 ) , psaA ( AB044235 ) , psaB ( AB044459 ) , psbC ( AB044515 AB044516 AB044517 ) , rbcL ( AB044170 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Phototaxis ; Heterothallic  
Other strain no. Other collection strain no. : UTEX 1394
 
Remarks Axenic 
Movie  
Reference
Nozaki, H., Mori, F., Tanaka, Y., Matsuzaki, R., Yamashita, S., Yamaguchi, H., Kawachi, K. 2023 Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene. BMC Microbiol., 23, 16 (article ID).
Keywords: Asexual cycle; Astrephomene; Cryopreservation; Cryoprotectant; Culture collection; Volvocine green algae
Strain(s): 4184195645656288538548554017 
PubMed: 36650459
DOI: 10.1186/s12866-023-02767-3

Nozaki, H., Misawa, K., Kajita, T., Kato, M., Nohara, S., Watanabe, M. M. 2000 Origin and evolution of the colonial Volvocales (Chlorophyceae) as inferred from multiple, chloroplast gene sequences. Mol. Phylogenet. Evol., 17, 256-268.
Strain(s): 418456460474541545564566569571572574577582653654666720721722724725727728730731732734735736737851854872882884886887888889890893895896898 
PubMed: 11083939
DOI: 10.1006/mpev.2000.0831

Brooks, A. E. 1966 The sexual cycle and intercrossing in the genus Astrephomene. J. Protozool., 13, 367-375.
Strain(s): 853854855 
DOI: 10.1111/j.1550-7408.1966.tb01922.x

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